Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,439,141 C→T *123* (TAG→TAA)  yhdN ← DUF1992 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,439,1410CT100.0% 321.3 / NA 98*123* (TAG→TAA) yhdNDUF1992 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (49/49);  total (49/49)

GGTATATAGGCGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCACTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCG  >  NC_000913/3439008‑3439276
                                                                                                                                     |                                                                                                                                       
ggTATATAGGCGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGtt                                                                                                                                    <  1:145630/139‑1 (MQ=255)
      taGGCGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGtt                                                                                                                              >  2:550706/1‑139 (MQ=255)
         gCGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGAt                                                                                                                           >  2:987406/1‑139 (MQ=255)
          cGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGAtt                                                                                                                          <  2:2001792/139‑1 (MQ=255)
          cGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGAtt                                                                                                                          >  2:1506363/1‑139 (MQ=255)
            aaaCCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGAtttt                                                                                                                        >  1:504/1‑139 (MQ=255)
                           aCGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTc                                                                                                         <  1:550706/139‑1 (MQ=255)
                              cACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGc                                                                                                      >  1:885912/1‑139 (MQ=255)
                                 ttCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATa                                                                                                   >  2:779135/1‑139 (MQ=255)
                                  tCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATaa                                                                                                  >  2:1604045/1‑139 (MQ=255)
                                    aTGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCG‑‑‑‑ATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATcgcc                                                                                             >  1:723931/1‑138 (MQ=255)
                                    aTGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCG‑‑‑‑ATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATcgcc                                                                                             <  2:723931/138‑1 (MQ=255)
                                       cTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATcgcc                                                                                             <  1:19999/139‑1 (MQ=255)
                                         ccATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATcgccgc                                                                                           >  2:377178/1‑139 (MQ=255)
                                           atcatcTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGt                                                                                         >  2:620633/1‑139 (MQ=255)
                                               tcTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCTCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTaaaa                                                                                     <  1:987406/139‑1 (MQ=255)
                                                 tgctgTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACtt                                                                                                            >  1:1890034/1‑114 (MQ=255)
                                                 tgctgTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACtt                                                                                                            <  2:1890034/114‑1 (MQ=255)
                                                  gctgTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAAt                                                                                  <  2:338480/139‑1 (MQ=255)
                                                    tgTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCg                                                                                <  2:1057426/139‑1 (MQ=255)
                                                      tttttCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGt                                                                              >  1:712375/1‑139 (MQ=255)
                                                         ttCGTAATAACGAATCGTGTCGGGTGTTCCTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACt                                                                           <  1:1836299/139‑1 (MQ=255)
                                                         ttCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACt                                                                           <  1:1604045/139‑1 (MQ=255)
                                                         ttCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACt                                                                           <  1:620633/139‑1 (MQ=255)
                                                         ttCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACt                                                                           <  2:885912/139‑1 (MQ=255)
                                                                 aaCGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAg                                                                   <  1:779135/139‑1 (MQ=255)
                                                                     aaTCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGt                                                                                                                               >  2:286249/1‑75 (MQ=255)
                                                                     aaTCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGt                                                                                                                               <  1:286249/75‑1 (MQ=255)
                                                                      aTCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTAt                                                                                                                                  <  1:1919334/71‑1 (MQ=255)
                                                                      aTCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTAt                                                                                                                                  >  2:1919334/1‑71 (MQ=255)
                                                                       tCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTc                                                             >  1:528713/1‑139 (MQ=255)
                                                                        cGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGAtt                                                                                                                          >  1:260838/1‑77 (MQ=255)
                                                                        cGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGAtt                                                                                                                          <  2:260838/77‑1 (MQ=255)
                                                                         gtgtCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGc                                                           <  2:2002741/139‑1 (MQ=255)
                                                                          tgtCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTaa                                                                                       <  1:832927/110‑1 (MQ=255)
                                                                          tgtCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTaa                                                                                       >  2:832927/1‑110 (MQ=255)
                                                                          tgtCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCa                                                          >  1:289459/1‑139 (MQ=255)
                                                                          tgtCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCa                                                          >  1:1281949/1‑139 (MQ=255)
                                                                            tCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGc                                                        >  1:420420/1‑139 (MQ=255)
                                                                                  gtTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAAt                                                  <  2:1281949/139‑1 (MQ=255)
                                                                                   ttACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGtt                                                                                                                                    <  2:170367/56‑1 (MQ=255)
                                                                                   ttACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGtt                                                                                                                                    >  1:170367/1‑56 (MQ=255)
                                                                                   ttACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTc                                                                                                         <  2:1520153/83‑1 (MQ=255)
                                                                                   ttACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTc                                                                                                         >  1:1520153/1‑83 (MQ=255)
                                                                                   ttACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAAtt                                                 <  2:712375/139‑1 (MQ=255)
                                                                                         ccGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAAtt                                                 <  2:1338090/133‑1 (MQ=255)
                                                                                         ccGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTa                                           >  2:560974/1‑139 (MQ=255)
                                                                                         ccGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGCCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAAtt                                                 >  1:1338090/1‑133 (MQ=255)
                                                                                         ccGCCATTTTTGCCAGCTCACCAATGCGATACATACATAC‑CCATTAGTTATCGTTGATTTTGTcc                                                                                                                    <  2:1151454/65‑1 (MQ=255)
                                                                                         ccGCCATTTTTGCCAGCTCACCAATGCGATACATACATAC‑CCATTAGTTATCGTTGATTTTGTcc                                                                                                                    >  1:1151454/1‑65 (MQ=255)
                                                                                          cGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACtt                                                                                                            >  2:615074/1‑73 (MQ=255)
                                                                                          cGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACtt                                                                                                            <  1:615074/73‑1 (MQ=255)
                                                                                          cGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTaa                                          >  2:293830/1‑139 (MQ=255)
                                                                                           gCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGAtttt                                                                                                                        >  2:707927/1‑60 (MQ=255)
                                                                                           gCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGAtttt                                                                                                                        <  1:707927/60‑1 (MQ=255)
                                                                                           gCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTc                                                                                                         >  1:1178128/1‑75 (MQ=255)
                                                                                           gCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTc                                                                                                         <  2:1178128/75‑1 (MQ=255)
                                                                                           gCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAAt                                         >  1:1693346/1‑139 (MQ=255)
                                                                                                 tttGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAAt                                   >  2:795103/1‑139 (MQ=255)
                                                                                                   tGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCg                                 <  2:289459/139‑1 (MQ=255)
                                                                                                    gCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGa                                >  1:1275882/1‑139 (MQ=255)
                                                                                                         cTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGATGGCt                           <  1:377178/139‑1 (MQ=255)
                                                                                                         cTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCt                           <  1:560974/139‑1 (MQ=255)
                                                                                                           cACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTaa                         <  1:1144135/139‑1 (MQ=255)
                                                                                                                aTGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCATCTTCAATTCCAGTAATGACAATCGACGGCTAACctct                    <  2:1883394/139‑1 (MQ=255)
                                                                                                                   cGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCtt                                                      <  1:1612435/102‑1 (MQ=255)
                                                                                                                   cGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCtt                                                      >  2:1612435/1‑102 (MQ=255)
                                                                                                                   cGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGa                 <  1:293830/139‑1 (MQ=255)
                                                                                                                      tacatacatacTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGatat              >  1:1175093/1‑139 (MQ=255)
                                                                                                                        catacatacTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCTCGTAAAAAATCGGTACTCAATCCAGCTTGTCTCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTg            <  1:795103/139‑1 (MQ=255)
                                                                                                                        catacatacTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATc                                                                      >  1:1090398/1‑81 (MQ=255)
                                                                                                                        catacatacTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATc                                                                      <  2:1090398/81‑1 (MQ=255)
                                                                                                                        catacatacTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGa                                       <  2:1729056/112‑1 (MQ=255)
                                                                                                                        catacatacTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGa                                       >  1:1729056/1‑112 (MQ=255)
                                                                                                                           acatacTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCgg         >  1:1016723/1‑139 (MQ=255)
                                                                                                                               acTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGt                                                                                                          >  1:273244/1‑38 (MQ=255)
                                                                                                                               acTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGt                                                                                                          <  2:273244/38‑1 (MQ=255)
                                                                                                                               acTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATGATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGatca     >  1:1451597/1‑139 (MQ=255)
                                                                                                                               acTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACt                                                                           >  1:164547/1‑69 (MQ=255)
                                                                                                                               acTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACt                                                                           <  2:164547/69‑1 (MQ=255)
                                                                                                                                cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTTATCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCg          <  1:1334337/133‑1 (MQ=255)
                                                                                                                                cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACt                                                                           <  2:1341584/68‑1 (MQ=255)
                                                                                                                                cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACt                                                                           >  1:1341584/1‑68 (MQ=255)
                                                                                                                                cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCt                           <  1:2013831/116‑1 (MQ=255)
                                                                                                                                cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCt                           >  2:2013831/1‑116 (MQ=255)
                                                                                                                                cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTaa                         <  2:1593596/118‑1 (MQ=255)
                                                                                                                                cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTaa                         >  1:1593596/1‑118 (MQ=255)
                                                                                                                                cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATGTTGCg          >  2:1334337/1‑133 (MQ=255)
                                                                                                                                cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGatcat    <  2:528713/139‑1 (MQ=255)
                                                                                                                                cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGatcat    >  2:1532967/1‑139 (MQ=255)
                                                                                                                                cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGatcat    >  1:1847885/1‑139 (MQ=255)
                                                                                                                                 tCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTc                                                     <  1:1811649/89‑1 (MQ=255)
                                                                                                                                 tCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTc                                                     >  2:1811649/1‑89 (MQ=255)
                                                                                                                                 tCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGatcatc   <  2:1175093/139‑1 (MQ=255)
                                                                                                                                 tCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGatcatc   <  2:1451597/139‑1 (MQ=255)
                                                                                                                                  ccATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAAtt                                                 <  2:482031/92‑1 (MQ=255)
                                                                                                                                  ccATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAAtt                                                 >  1:482031/1‑92 (MQ=255)
                                                                                                                                  ccATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCg  >  1:1717115/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                       
GGTATATAGGCGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCACTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCG  >  NC_000913/3439008‑3439276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑