Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	35,373	G→C	intergenic (‑2/+4)	caiE ← / ← caiD	stimulator of CaiD and CaiB enzyme activities/carnitinyl‑CoA dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	35,373	0	G	C	100.0%	291.8 / NA	81	intergenic (‑2/+4)	caiE/caiD	stimulator of CaiD and CaiB enzyme activities/carnitinyl‑CoA dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (39/42);  total (39/42)

ACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGGTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATACGCTTCTTCT  >  NC_000913/35240‑35506                                                                                                                                      |                                                                                                                                      acGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGcttgtt                                                                                                                                  <  2:905412/139‑1 (MQ=255) acGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGcttgtt                                                                                                                                  <  2:1201514/139‑1 (MQ=255)    cAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAAc                                                                                                                               >  1:495581/1‑139 (MQ=255)     aGTGAGGCGAGT‑GGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGt                                                                                                                             >  2:1393406/1‑139 (MQ=255)       tGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTc                                                                                                                            <  2:2035456/139‑1 (MQ=255)        gAGGCGAGT‑GGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTTATAGCTCACGCTTGTTAACGTCCt                                                                                                                          <  1:1393406/139‑1 (MQ=255)         aGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCt                                                                                                                          <  1:407050/139‑1 (MQ=255)           gCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCttt                                                                                                                        <  2:434490/139‑1 (MQ=255)           gCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCttt                                                                                                                        <  2:1347941/139‑1 (MQ=255)               gTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCcac                                                                                                                    >  1:952246/1‑139 (MQ=255)                tGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCcaca                                                                                                                   <  1:1104442/139‑1 (MQ=255)                   gCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCg                                                                                                                <  2:523022/139‑1 (MQ=255)                    ccGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCacac                                                                                                                  >  2:1536930/1‑136 (MQ=255)                    ccGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCacac                                                                                                                  <  1:1536930/136‑1 (MQ=255)                             acacCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCttt                                                                                                                        >  2:409241/1‑121 (MQ=255)                             acacCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCttt                                                                                                                        <  1:409241/121‑1 (MQ=255)                               acCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTtct                                                                                                    <  2:135348/139‑1 (MQ=255)                               acCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTtct                                                                                                    <  2:2050456/139‑1 (MQ=255)                                 cGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTa                                                                                                                                 >  2:144270/1‑108 (MQ=255)                                 cGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTa                                                                                                                                 <  1:144270/108‑1 (MQ=255)                                 cGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCg                                                                                                  >  1:1281199/1‑139 (MQ=255)                                  ggCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCttt                                                                                                                        <  1:941315/116‑1 (MQ=255)                                  ggCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCttt                                                                                                                        >  2:941315/1‑116 (MQ=255)                                  ggCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCgg                                                                                                 >  2:1950844/1‑139 (MQ=255)                                    cTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCa                                                                                               >  1:1659398/1‑139 (MQ=255)                                    cTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCa                                                                                               >  2:1221866/1‑139 (MQ=255)                                      cccACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCaaa                                                                                             <  1:548349/139‑1 (MQ=255)                                             tCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGc                                                                                      >  1:1719170/1‑139 (MQ=255)                                             tCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGc                                                                                      <  1:1721629/139‑1 (MQ=255)                                              cacaTCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTc                                                                                                                            >  1:1436595/1‑100 (MQ=255)                                              cacaTCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTc                                                                                                                            <  2:1436595/100‑1 (MQ=255)                                                  tCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGccc                                                                                 <  1:1950844/139‑1 (MQ=255)                                                             gACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCAt                                                                      <  1:451063/139‑1 (MQ=255)                                                                  cACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGc                                                                                   <  2:1616890/121‑1 (MQ=255)                                                                  cACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGc                                                                                   >  1:1616890/1‑121 (MQ=255)                                                                     tGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGaa                                                              >  2:1539623/1‑139 (MQ=255)                                                                       ggATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATcg                                                                                                           <  1:1005052/92‑1 (MQ=255)                                                                       ggATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATcg                                                                                                           >  2:1005052/1‑92 (MQ=255)                                                                       ggATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATg                                                            >  2:1164717/1‑139 (MQ=255)                                                                            gACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGccc                                                                                 >  1:1475149/1‑113 (MQ=255)                                                                            gACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGccc                                                                                 <  2:1475149/113‑1 (MQ=255)                                                                            gACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGaa                                                       >  2:170801/1‑139 (MQ=255)                                                                            gACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGaa                                                       >  1:352208/1‑139 (MQ=255)                                                                                 aCGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAAc                                                                                                                               >  2:314629/1‑62 (MQ=255)                                                                                 aCGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAAc                                                                                                                               <  1:314629/62‑1 (MQ=255)                                                                                 aCGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCg                                                    >  2:528911/1‑137 (MQ=255)                                                                                 aCGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCg                                                    <  1:528911/137‑1 (MQ=255)                                                                                 aCGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGAt                                                  >  1:1708285/1‑139 (MQ=255)                                                                                     cGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGAt                                              <  1:1164717/139‑1 (MQ=255)                                                                                      gTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATa                                             >  2:1133919/1‑139 (MQ=255)                                                                                       tCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCg                                                                                     >  2:617452/1‑98 (MQ=255)                                                                                       tCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCg                                                                                     <  1:617452/98‑1 (MQ=255)                                                                                              aaCCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCttt                                                                                                                        >  2:253460/1‑56 (MQ=255)                                                                                              aaCCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCttt                                                                                                                        <  1:253460/56‑1 (MQ=255)                                                                                               accaccGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGAt                                              >  1:142222/1‑129 (MQ=255)                                                                                               accaccGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGAt                                              <  2:142222/129‑1 (MQ=255)                                                                                                 caccGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAaca                                  <  2:1659398/139‑1 (MQ=255)                                                                                                   ccGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACAcg                                >  1:1683004/1‑139 (MQ=255)                                                                                                   ccGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACAcg                                <  1:1539623/139‑1 (MQ=255)                                                                                                   ccGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACAcg                                <  2:352208/139‑1 (MQ=255)                                                                                                    cGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACAcg                                >  2:1644717/1‑138 (MQ=255)                                                                                                    cGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACAcg                                <  1:1644717/138‑1 (MQ=255)                                                                                                       aaTTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACAcgccgc                            <  2:1708285/139‑1 (MQ=255)                                                                                                          tAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGc                         <  1:170801/139‑1 (MQ=255)                                                                                                           aaCCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGGTAGTGTTTCAACACGCCGCTGCg                        >  1:130297/1‑139 (MQ=255)                                                                                                            aCCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGGTAGTGTTTCAACACGCCGCTGCGa                       <  2:130297/139‑1 (MQ=255)                                                                                                             ccccTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGaa                      >  2:727539/1‑139 (MQ=255)                                                                                                              cccTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTg                          >  1:244457/1‑134 (MQ=255)                                                                                                              cccTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTg                          <  2:244457/134‑1 (MQ=255)                                                                                                                ctccagggCGTAATAGCTGACGCTTGTTAACGTCCTGTCCACGCCGGATCGCGCCTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCAGCGAatat                   <  1:517444/133‑1 (MQ=255)                                                                                                                 tCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTcc                                                                 <  2:773698/92‑1 (MQ=255)                                                                                                                 tCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTcc                                                                 >  1:773698/1‑92 (MQ=255)                                                                                                                  cAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAg                 <  1:1344827/139‑1 (MQ=255)                                                                                                                       gCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGAt                                              <  1:1186200/105‑1 (MQ=255)                                                                                                                       gCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGAt                                              >  2:1186200/1‑105 (MQ=255)                                                                                                                        cGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTcc                                                                 >  1:1836012/1‑85 (MQ=255)                                                                                                                        cGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTcc                                                                 <  2:1836012/85‑1 (MQ=255)                                                                                                                        cGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGaa                                                       >  2:645166/1‑95 (MQ=255)                                                                                                                        cGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGaa                                                       <  1:645166/95‑1 (MQ=255)                                                                                                                          tAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAg                                                         <  2:1750923/91‑1 (MQ=255)                                                                                                                          tAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAg                                                         >  1:1750923/1‑91 (MQ=255)                                                                                                                             tAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGc                                                           <  2:2025193/86‑1 (MQ=255)                                                                                                                             tAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGc                                                           >  1:2025193/1‑86 (MQ=255)                                                                                                                                 tCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATACGCTtcttct  >  2:236017/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                      ACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGGTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATACGCTTCTTCT  >  NC_000913/35240‑35506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑