Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,145	C→T	A234T (GCC→ACC)	bioH ←	pimeloyl‑ACP methyl ester carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,145	0	C	T	100.0%	189.6 / NA	59	A234T (GCC→ACC)	bioH	pimeloyl‑ACP methyl ester carboxylesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/33);  total (26/33)

TCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACG  >  NC_000913/3544013‑3544280                                                                                                                                     |                                                                                                                                        tCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCttt                                                                                                                                   >  1:793847/1‑139 (MQ=255)   agaagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTgg                                                                                                                                 <  1:1811703/139‑1 (MQ=255)     aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                               <  1:885604/139‑1 (MQ=255)     aagaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                               <  1:1158334/139‑1 (MQ=255)       gaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGaa                                                                                                                             <  2:177797/139‑1 (MQ=255)       gaagaGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGaa                                                                                                                             >  1:1641260/1‑139 (MQ=255)           agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCATCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTgg                                                                                                                                 >  2:1366722/1‑131 (MQ=255)           agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCATCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTgg                                                                                                                                 <  1:1366722/131‑1 (MQ=255)           agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                               >  1:338242/1‑133 (MQ=255)           agTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                               <  2:338242/133‑1 (MQ=255)                  tGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGAtt                                                                                                                  >  1:265182/1‑139 (MQ=255)                        gAGTCCGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTTACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCtgt                                                                                                            <  2:1277856/139‑1 (MQ=255)                                       tGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCtgt                                                                                                            <  1:389210/124‑1 (MQ=255)                                       tGTCTCGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCtgt                                                                                                            >  2:389210/1‑124 (MQ=255)                                         tctcGCCATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATcc                                                                                           <  2:793847/139‑1 (MQ=255)                                              ccATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                               >  2:1390478/1‑98 (MQ=255)                                              ccATTTCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                               <  1:1390478/98‑1 (MQ=255)                                                  ttCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                               <  1:1761231/94‑1 (MQ=255)                                                  ttCAAAAGCCACTTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                               >  2:1761231/1‑94 (MQ=255)                                                            acttACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                               >  2:1585958/4‑84 (MQ=255)                                                            acttACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                               <  1:1585958/81‑1 (MQ=255)                                                             cttACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTgcg                                                                       <  2:265182/137‑1 (MQ=255)                                                             cttACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTgcg                                                                       <  1:145801/137‑1 (MQ=255)                                                                   cccTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTgcg                                                                       <  2:846999/133‑1 (MQ=255)                                                                   cccTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTgcg                                                                       >  1:846999/1‑133 (MQ=255)                                                                    ccTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCAcc                                                                >  1:218963/1‑139 (MQ=255)                                                                      tctGCTTCGACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGa                                                                                                                              <  2:985216/75‑1 (MQ=255)                                                                      tctGCTTCGACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGa                                                                                                                              >  1:985216/1‑75 (MQ=255)                                                                      tctGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAg                                                              <  2:1760218/139‑1 (MQ=255)                                                                      tctGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAg                                                              <  1:1073356/139‑1 (MQ=255)                                                                       ctGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGc                                                                                                                                <  2:1421733/72‑1 (MQ=255)                                                                       ctGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGc                                                                                                                                <  2:1588545/72‑1 (MQ=255)                                                                       ctGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGc                                                                                                                                >  1:1588545/1‑72 (MQ=255)                                                                       ctGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGc                                                                                                                                >  1:1421733/1‑72 (MQ=255)                                                                        tGCTTCAACGCCACCAGCAGGTGACAAAACTCGGTCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGcc                                                                                                      <  2:233938/97‑1 (MQ=255)                                                                        tGCTTCAACGCCACCAGCAGGTGACAAAACTCGGTCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGcc                                                                                                      >  1:233938/1‑97 (MQ=255)                                                                        tGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGAATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGcc                                                                                                      >  1:441802/1‑97 (MQ=255)                                                                        tGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGAATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGcc                                                                                                      <  2:441802/97‑1 (MQ=255)                                                                          cTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                               >  2:140967/1‑70 (MQ=255)                                                                          cTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                               <  1:140967/70‑1 (MQ=255)                                                                            tCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTc                                                        >  1:896709/1‑139 (MQ=255)                                                                              aaCGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCga                                                      <  2:218963/139‑1 (MQ=255)                                                                                cGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                               >  2:531659/1‑64 (MQ=255)                                                                                cGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                               <  1:531659/64‑1 (MQ=255)                                                                                cGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGa                                                                                                                              >  2:1716684/1‑65 (MQ=255)                                                                                cGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGa                                                                                                                              <  1:1716684/65‑1 (MQ=255)                                                                                cGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCgaga                                                    <  2:1641260/139‑1 (MQ=255)                                                                                cGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCgaga                                                    >  1:230688/1‑139 (MQ=255)                                                                                  ccaccaGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                               <  2:1679762/62‑1 (MQ=255)                                                                                  ccaccaGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCg                                                                                                                               >  1:1679762/1‑62 (MQ=255)                                                                                    accaGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGAtt                                                                                                                  >  2:1905686/1‑73 (MQ=255)                                                                                    accaGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGTCGCTTTGGCGAAGATATATGAtt                                                                                                                  <  1:1905686/73‑1 (MQ=255)                                                                                                                            gcgcATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCg                                                          >  2:1447545/1‑89 (MQ=255)                                                                                                                            gcgcATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCg                                                          <  1:1447545/89‑1 (MQ=255)                                                                                                                               cATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCgaga                                                    <  2:1096627/92‑1 (MQ=255)                                                                                                                               cATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCgaga                                                    >  1:1096627/1‑92 (MQ=255)                                                                                                                               cATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCg                                      <  2:1527957/106‑1 (MQ=255)                                                                                                                               cATGGGTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCg                                      >  1:1527957/1‑106 (MQ=255)                                                                                                                                  gggTCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACg  <  2:896709/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        TCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAAAACTCGGCCGGATGCGAAATAAATGGCGCATGGGCCGCTTTGGCGAAGATATATGATTCGCTGTGAGGCCAAAGTTTATCCAGCATCGGCACCACTTTGCGCGGCACCAGACCGTCGAGATAGCCATACAATCGCAAAAACGGCATGGACACGTTTTGCAGCGGCTGACG  >  NC_000913/3544013‑3544280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑