Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,615,448	A→G	T70A (ACC→GCC)	nikB →	nickel ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,615,448	0	A	G	100.0%	167.1 / NA	48	T70A (ACC→GCC)	nikB	nickel ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/28);  total (20/28)

TCTGATGCTGCGCCTCGGTACCGGCGACCCGGCGCTCGATTATTTGCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCACCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGTGCGGCGCTGGTATTAATTCTGCT  >  NC_000913/3615312‑3615581                                                                                                                                         |                                                                                                                                      tCTGATGCTGCGCCTCGGTACCGGCGACCCGGCGCTCGATTATTTGCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGcc                                                                                                                                     <  1:588091/139‑1 (MQ=255)     aTGCTGCGCCTCGGTACCGGCGACCCGGCGCTCGATTATTTGCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGt                                                                                                                                 >  2:1228834/1‑139 (MQ=255)            gcCTCGGTACCGGCGACCCGGCGCTCGATTATTTGCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGaa                                                                                                                          <  2:327999/139‑1 (MQ=255)                           aCCCGGCGCTCGATTATTTGCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGcc                                                                                                                                     >  2:979669/1‑113 (MQ=255)                           aCCCGGCGCTCGATTATTTGCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGcc                                                                                                                                     <  1:979669/113‑1 (MQ=255)                            cccGGCGCTCGATTATTTGCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGAc                                                                                                          <  2:1743560/139‑1 (MQ=255)                                              gCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCgcc                                                                                        >  1:251458/1‑139 (MQ=255)                                                gtctgtctAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGcc                                                                                                                                     <  2:1210149/92‑1 (MQ=255)                                                gtctgtctAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGcc                                                                                                                                     >  1:1210149/1‑92 (MQ=255)                                                     tctAATCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAAc                                                                                 >  1:971669/1‑139 (MQ=255)                                                        aaCCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGcc                                                                              <  1:1640732/139‑1 (MQ=255)                                                        aaCCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGcc                                                                              <  2:251458/139‑1 (MQ=255)                                                               cgccgACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACt                                                                       >  1:1893674/1‑139 (MQ=255)                                                               cgccgACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACt                                                                       >  1:1532198/1‑139 (MQ=255)                                                                  cgACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGa                                                                    <  1:248289/139‑1 (MQ=255)                                                                    aCGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACg                                                                  <  1:1228834/139‑1 (MQ=255)                                                                        cGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGatat                                                              >  1:1848646/1‑139 (MQ=255)                                                                                  ggCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGAc                                                                                                          >  2:2022010/1‑85 (MQ=255)                                                                                  ggCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGAc                                                                                                          <  1:2022010/85‑1 (MQ=255)                                                                                  ggCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTc                                                    >  2:1311183/1‑139 (MQ=255)                                                                                     ctctACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTg                                                 <  2:586564/139‑1 (MQ=255)                                                                                              cACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACg                                        >  2:949028/1‑139 (MQ=255)                                                                                               aCCATTCTGGGACTGGATCAGCCGCGGTACGGCCAGTACTGCGCCTGGTGGTGGAAGTCGCCGCATCTTGACTTTGTTATCTCATTCGCCAGCCAACGCCCGGAACTGGACGAGTTGCGGAACTTCCTGCCCCCCACGc                                       >  1:486277/1‑139 (MQ=255)                                                                                               aCCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGc                                       <  1:1385582/139‑1 (MQ=255)                                                                                               aCCATGCTGGGACTGGAACAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGc                                       >  1:1176867/1‑139 (MQ=255)                                                                                                  aTGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCgcc                                                                                        <  1:482666/87‑1 (MQ=255)                                                                                                  aTGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCgcc                                                                                        >  2:482666/1‑87 (MQ=255)                                                                                                   tGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGa                                   <  2:971669/139‑1 (MQ=255)                                                                                                          aCTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTa                                                                         >  1:969888/1‑94 (MQ=255)                                                                                                          aCTGGATCAGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTa                                                                         <  2:969888/94‑1 (MQ=255)                                                                                                                  aGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGcc                                                                              >  1:235170/1‑81 (MQ=255)                                                                                                                  aGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGcc                                                                              <  1:189290/81‑1 (MQ=255)                                                                                                                  aGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGcc                                                                              <  2:235170/81‑1 (MQ=255)                                                                                                                  aGCCGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGcc                                                                              >  2:189290/1‑81 (MQ=255)                                                                                                                    ccGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGa                                                                                                           >  2:1802680/1‑50 (MQ=255)                                                                                                                    ccGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGa                                                                                                           <  1:1802680/50‑1 (MQ=255)                                                                                                                    ccGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTg                              <  2:1975483/127‑1 (MQ=255)                                                                                                                    ccGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTg                              >  1:1975483/1‑127 (MQ=255)                                                                                                                     cGCTGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGTGCGGCGCt                 <  2:1532198/139‑1 (MQ=255)                                                                                                                        tGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACt                                                      <  2:327264/99‑1 (MQ=255)                                                                                                                        tGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACt                                                      >  1:327264/1‑99 (MQ=255)                                                                                                                        tGTACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGTGCGGCGCTGGt              <  2:1597365/139‑1 (MQ=255)                                                                                                                          tACGTCCAGTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGTGCGGCGCTGGTAt            <  2:1176867/139‑1 (MQ=255)                                                                                                                                  gTACGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGATGGAACGTGCAGGTGCGGCGCTGGTATTAATTCTg    <  2:486277/139‑1 (MQ=255)                                                                                                                                    aCGGCGCCTGGTTGTGGAAGGCGCTGTATCTTGACTTAGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGTGCGGCGCTGGTATTAATTCTGCt  <  1:1311183/139‑1 (MQ=255)                                                                                                                                    aCGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGTGCGGCGCTGGTATTAATTCTGCt  <  2:1848646/139‑1 (MQ=255)                                                                                                                                    aCGGCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGTGCGGCGCTGGTATTAATTCTGCt  <  2:1782442/139‑1 (MQ=255)                                                                                                                                      ggCGCCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGTgcggcg                   <  1:101087/120‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TCTGATGCTGCGCCTCGGTACCGGCGACCCGGCGCTCGATTATTTGCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCACCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGTGCGGCGCTGGTATTAATTCTGCT  >  NC_000913/3615312‑3615581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑