Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,683,630	C→T	*663* (TAG→TAA)	yhjK ←	cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,683,630	0	C	T	100.0%	298.3 / NA	91	*663* (TAG→TAA)	yhjK	cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (48/43);  total (48/43)

CCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGCTTCAGTCTCCACGCCT  >  NC_000913/3683494‑3683756                                                                                                                                         |                                                                                                                               ccTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTa                                                                                                                              >  1:1107430/1‑139 (MQ=255)    gCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACtt                                                                                                                           >  1:1984196/1‑139 (MQ=255)    gCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACtt                                                                                                                           <  1:1561116/139‑1 (MQ=255)       gCCTTAAAATAACACCTAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTttc                                                                                                                        >  2:497896/1‑139 (MQ=255)       gCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTttc                                                                                                                        >  2:10154/1‑139 (MQ=255)         cTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGTGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTttctt                                                                                                                      <  2:1107430/139‑1 (MQ=255)                 aaCACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTaa                                                                                                              <  2:1154795/139‑1 (MQ=255)                  acacCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAAc                                                                                                             >  2:1875118/1‑139 (MQ=255)                      ccAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAActctc                                                                                                         >  1:692845/1‑139 (MQ=255)                        aGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCtt                                                                                                       >  2:812530/1‑139 (MQ=255)                          acaacaTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCg                                                                                                     <  1:171615/139‑1 (MQ=255)                            aacaTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGaa                                                                                                   <  1:812530/139‑1 (MQ=255)                            aacaTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGaa                                                                                                   <  2:1688477/139‑1 (MQ=255)                              caTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGaa                                                                                                   >  1:1462571/1‑137 (MQ=255)                              caTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGaa                                                                                                   <  1:1349231/137‑1 (MQ=255)                              caTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGaa                                                                                                   <  2:1462571/137‑1 (MQ=255)                              caTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGaa                                                                                                   >  2:1349231/1‑137 (MQ=255)                              caTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGa                                                                                                 <  1:1475331/139‑1 (MQ=255)                               aTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGAt                                                                                                >  1:1039376/1‑139 (MQ=255)                                tCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGTTAACTCTCTTCGAAGAtt                                                                                               >  1:1667837/1‑139 (MQ=255)                                tCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGAtt                                                                                               <  2:1984196/139‑1 (MQ=255)                                tCACAGAAATGTAACTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGAtt                                                                                               <  1:1070777/139‑1 (MQ=255)                                    aGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCaa                                                                                           <  2:1039376/139‑1 (MQ=255)                                        aTGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTttctt                                                                                                                      >  2:884139/1‑108 (MQ=255)                                        aTGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTttctt                                                                                                                      <  1:884139/108‑1 (MQ=255)                                             ccTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTg                                                                                  >  1:454206/1‑139 (MQ=255)                                              cTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTgg                                                                                 >  2:683521/1‑139 (MQ=255)                                                  aTCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTttctt                                                                                                                      >  2:812651/1‑98 (MQ=255)                                                  aTCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTttctt                                                                                                                      <  1:812651/98‑1 (MQ=255)                                                  aTCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGa                                                                             >  1:268870/1‑139 (MQ=255)                                                     aTAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCa                                                                          >  2:766140/1‑139 (MQ=255)                                                      tAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTttctt                                                                                                                      >  2:10189/1‑94 (MQ=255)                                                      tAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTttctt                                                                                                                      <  1:10189/94‑1 (MQ=255)                                                       aaTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTaa                                                                                                              >  2:799822/1‑101 (MQ=255)                                                       aaTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTaa                                                                                                              <  1:799822/101‑1 (MQ=255)                                                       aaTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCaaa                                                                        <  1:10154/139‑1 (MQ=255)                                                        aTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAAc                                                                                                             <  2:1824150/101‑1 (MQ=255)                                                        aTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAAc                                                                                                             >  1:1824150/1‑101 (MQ=255)                                                           gAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCg                                                                                                     <  2:466040/106‑1 (MQ=255)                                                           gAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCg                                                                                                     >  1:466040/1‑106 (MQ=255)                                                           gAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAgg                                                                    <  1:766140/139‑1 (MQ=255)                                                           gAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAgg                                                                    <  2:1378366/139‑1 (MQ=255)                                                                aaaaaGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGcc                                                               <  2:843625/139‑1 (MQ=255)                                                                aaaaaGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGcc                                                               <  2:454206/139‑1 (MQ=255)                                                                 aaaaGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGccc                                                              <  1:497896/139‑1 (MQ=255)                                                                         aaaaaGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAAt                                                      >  1:1672121/1‑139 (MQ=255)                                                                         aaaaaGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAAt                                                      >  1:865409/1‑139 (MQ=255)                                                                           aaaGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATAc                                                    <  1:839426/139‑1 (MQ=255)                                                                                ttGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACg                                               >  2:67892/1‑139 (MQ=255)                                                                                ttGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACg                                               >  2:62403/1‑139 (MQ=255)                                                                                ttGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACg                                               >  1:461661/1‑139 (MQ=255)                                                                                        gCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAg                                                                            <  2:1401447/102‑1 (MQ=255)                                                                                        gCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAg                                                                            >  1:1401447/1‑102 (MQ=255)                                                                                             cacaAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAAct                                                                                                            <  1:332112/65‑1 (MQ=255)                                                                                             cacaAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAAct                                                                                                            >  2:332112/1‑65 (MQ=255)                                                                                             cacaAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTgcca                                  >  1:715948/1‑139 (MQ=255)                                                                                                  aCCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAAc                                                                                                             >  2:651099/1‑59 (MQ=255)                                                                                                  aCCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAAc                                                                                                             <  1:651099/59‑1 (MQ=255)                                                                                                  aCCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGccagccag                             >  1:353888/1‑139 (MQ=255)                                                                                                  aCCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGACCTGGGCAATACCAACGCCCGCTTTTGccagccag                             >  2:1836532/1‑139 (MQ=255)                                                                                                            tttAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGa                                                                                                 <  1:1589050/61‑1 (MQ=255)                                                                                                            tttAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGa                                                                                                 >  2:1589050/1‑61 (MQ=255)                                                                                                            tttAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTgccagc                                >  2:1039182/1‑126 (MQ=255)                                                                                                            tttAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTgccagc                                <  1:1039182/126‑1 (MQ=255)                                                                                                            tttAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTtgtg                   <  2:1672121/139‑1 (MQ=255)                                                                                                            tttAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTtgtg                   >  1:17544/1‑139 (MQ=255)                                                                                                            tttAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTtgtg                   >  1:898595/1‑139 (MQ=255)                                                                                                              tAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGa                                                                                                 >  1:498872/1‑59 (MQ=255)                                                                                                              tAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGa                                                                                                 <  2:498872/59‑1 (MQ=255)                                                                                                                 aGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGCTTCa              <  2:715948/139‑1 (MQ=255)                                                                                                                 aGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGCGTCa              <  1:1875118/139‑1 (MQ=255)                                                                                                                      tGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAgg                                                                    >  1:1008398/1‑80 (MQ=255)                                                                                                                      tGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAgg                                                                    <  2:1008398/80‑1 (MQ=255)                                                                                                                      tGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGa                                                                   >  2:1809478/1‑81 (MQ=255)                                                                                                                      tGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGa                                                                   <  1:1809478/81‑1 (MQ=255)                                                                                                                      tGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCa                                                        <  1:1497899/92‑1 (MQ=255)                                                                                                                      tGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCa                                                        >  2:1497899/1‑92 (MQ=255)                                                                                                                      tGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATAc                                                    <  2:1913802/96‑1 (MQ=255)                                                                                                                      tGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATAc                                                    >  1:1913802/1‑96 (MQ=255)                                                                                                                      tGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGCTTCAGtctc         >  1:1739736/1‑139 (MQ=255)                                                                                                                             aGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGCTTCAGTCTCCACGCCt  >  2:529308/1‑139 (MQ=255)                                                                                                                               tttGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGAtt                                                                                               <  2:614715/44‑1 (MQ=255)                                                                                                                               tttGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGAtt                                                                                               >  1:614715/1‑44 (MQ=255)                                                                                                                               tttGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAgg                                                                    >  1:841999/1‑71 (MQ=255)                                                                                                                               tttGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAgg                                                                    <  2:841999/71‑1 (MQ=255)                                                                                                                               tttGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTc                           <  2:1416252/112‑1 (MQ=255)                                                                                                                               tttGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTc                           >  1:1416252/1‑112 (MQ=255)                                                                                                                                ttGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCaa                                                       >  2:1615007/1‑83 (MQ=255)                                                                                                                                ttGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCaa                                                       <  1:1615007/83‑1 (MQ=255)                                                                                                                                  ggggTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGa                                                                                                 <  2:743866/39‑1 (MQ=255)                                                                                                                                  ggggTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGa                                                                                                 >  1:743866/1‑39 (MQ=255)                                                                                                                                         |                                                                                                                               CCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGCTTCAGTCTCCACGCCT  >  NC_000913/3683494‑3683756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑