Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	344,967	C→T	H264H (CAC→CAT)	yahL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	344,967	0	C	T	100.0%	218.1 / NA	69	H264H (CAC→CAT)	yahL	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/33);  total (36/33)

CCTGAATCACTGGTGATGTTATTGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTT  >  NC_000913/344836‑345102                                                                                                                                    |                                                                                                                                        ccTGAATCACTGGTGATGTTATTGCTCTCTGTTCTGGACGT‑AAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAAt                                                                                                                                 >  1:474307/1‑139 (MQ=255)                   ttATTGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCtt                                                                                                                    >  1:442616/1‑135 (MQ=255)                   ttATTGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCtt                                                                                                                    <  2:442616/135‑1 (MQ=255)                       tGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAg                                                                                                            >  1:1079659/1‑139 (MQ=255)                           ctctGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAg                                                                                                        >  2:1591168/1‑139 (MQ=255)                               gTTCTGGACGT‑AAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTc                                                                                                   <  2:474307/139‑1 (MQ=255)                                          aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCtt                                                                                                                    <  1:62794/112‑1 (MQ=255)                                          aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCtt                                                                                                                    >  2:62794/1‑112 (MQ=255)                                               cATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTaaaa                                                                                                                                  <  2:170424/93‑1 (MQ=255)                                               cATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTaaaa                                                                                                                                  >  1:170424/1‑93 (MQ=255)                                                  aCAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                 <  2:1706516/139‑1 (MQ=255)                                                  aCAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                 >  1:1706516/1‑139 (MQ=255)                                                           atgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgag                                                                        >  2:1861284/1‑139 (MQ=255)                                                           atgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGATACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgag                                                                        >  2:1733150/1‑139 (MQ=255)                                                            tgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgaga                                                                       <  2:1079659/139‑1 (MQ=255)                                                              cgcgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCTCCTGAGAGt                                                                     >  2:1317401/1‑139 (MQ=255)                                                               gcgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                    <  1:1733150/139‑1 (MQ=255)                                                                cgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGa                                                                   >  2:1477826/1‑139 (MQ=255)                                                                  aTTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAAt                                                                                                                                 <  2:1103531/75‑1 (MQ=255)                                                                  aTTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAAt                                                                                                                                 >  1:1103531/1‑75 (MQ=255)                                                                    tAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCg                                                               <  1:1317401/139‑1 (MQ=255)                                                                       atgaatgGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCaa                                                            <  1:1477826/139‑1 (MQ=255)                                                                                          agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTaca                                                                                                                         <  2:379473/59‑1 (MQ=255)                                                                                          agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTaca                                                                                                                         >  1:379473/1‑59 (MQ=255)                                                                                          agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatc                                         <  1:1591168/139‑1 (MQ=255)                                                                                            tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTTTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCa                                                             <  1:2028968/117‑1 (MQ=255)                                                                                            tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTTTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCa                                                             >  2:2028968/1‑117 (MQ=255)                                                                                            tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                          >  2:1948474/1‑72 (MQ=255)                                                                                            tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                          <  1:1948474/72‑1 (MQ=255)                                                                                            tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTa                                                                                   >  2:1998175/1‑95 (MQ=255)                                                                                            tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTa                                                                                   <  1:1998175/95‑1 (MQ=255)                                                                                            tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAc                                                                             <  2:1975553/101‑1 (MQ=255)                                                                                            tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAc                                                                             >  1:1975553/1‑101 (MQ=255)                                                                                            tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                          >  1:509227/1‑136 (MQ=255)                                                                                            tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                          >  2:625573/1‑136 (MQ=255)                                                                                            tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                          <  1:625573/136‑1 (MQ=255)                                                                                            tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                          <  2:509227/136‑1 (MQ=255)                                                                                             gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                            <  1:1528808/133‑1 (MQ=255)                                                                                             gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                            >  2:1528808/1‑133 (MQ=255)                                                                                             gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgt                                      >  2:620093/1‑139 (MQ=255)                                                                                              cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                           >  1:1038021/1‑85 (MQ=255)                                                                                              cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                           <  2:1038021/85‑1 (MQ=255)                                                                                              cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                    <  1:864002/108‑1 (MQ=255)                                                                                              cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                    >  2:864002/1‑108 (MQ=255)                                                                                              cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                     >  2:69818/1‑139 (MQ=255)                                                                                                      aGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                           >  2:1532489/1‑77 (MQ=255)                                                                                                      aGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                           <  1:1532489/77‑1 (MQ=255)                                                                                                      aGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGt                                                     >  1:1941193/1‑115 (MQ=255)                                                                                                      aGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGt                                                     <  2:1941193/115‑1 (MQ=255)                                                                                                           aCAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCa                        >  2:205225/1‑139 (MQ=255)                                                                                                              gATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgaga                                                                       >  1:1050602/1‑89 (MQ=255)                                                                                                              gATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgaga                                                                       <  2:1050602/89‑1 (MQ=255)                                                                                                                     tCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                            <  1:1408429/109‑1 (MQ=255)                                                                                                                     tCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                            >  2:1408429/1‑109 (MQ=255)                                                                                                                         gTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                 >  2:599266/1‑68 (MQ=255)                                                                                                                         gTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                 <  1:599266/68‑1 (MQ=255)                                                                                                                         gTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                    <  2:897338/113‑1 (MQ=255)                                                                                                                         gTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                    >  1:897338/1‑113 (MQ=255)                                                                                                                          tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                    >  2:972837/1‑80 (MQ=255)                                                                                                                          tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                    <  1:2027304/80‑1 (MQ=255)                                                                                                                          tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                    <  1:972837/80‑1 (MQ=255)                                                                                                                          tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                    >  2:2027304/1‑80 (MQ=255)                                                                                                                          tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                          <  1:1579009/106‑1 (MQ=255)                                                                                                                          tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                          >  2:1579009/1‑106 (MQ=255)                                                                                                                          tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGcc                         >  1:935012/1‑123 (MQ=255)                                                                                                                          tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGcc                         <  2:935012/123‑1 (MQ=255)                                                                                                                               aaGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGc                                                              >  1:1546770/1‑81 (MQ=255)                                                                                                                               aaGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGc                                                              <  2:1546770/81‑1 (MQ=255)                                                                                                                                 gCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCtttttt  <  1:205225/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        CCTGAATCACTGGTGATGTTATTGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTT  >  NC_000913/344836‑345102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑