Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,819,488 C→T H205H (CAC→CAT) 
*561* (TAG→TAA) 		 yicG →
ligB ←		 UPF0126 family inner membrane protein
DNA ligase, NAD(+)‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,819,4880CT100.0% 282.6 / NA 86H205H (CAC→CAT) 
*561* (TAG→TAA) 		yicG
ligB		UPF0126 family inner membrane protein
DNA ligase, NAD(+)‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (47/39);  total (47/39)

ACATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAACTGCT  >  NC_000913/3819352‑3819621
                                                                                                                                        |                                                                                                                                     
aCATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTa                                                                                                                                     <  1:1034351/139‑1 (MQ=255)
    cgcgCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGt                                                                                                                                 >  2:904620/1‑139 (MQ=255)
    cgcgCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGt                                                                                                                                 >  2:444766/1‑139 (MQ=255)
    cgcgCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGt                                                                                                                                 >  1:1758585/1‑139 (MQ=255)
      cgcTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTc                                                                                                                               <  1:1718631/139‑1 (MQ=255)
      cgcTGCAACACTATGTTTCTAACCATGATGTAGTAATTATCTCCTCCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTc                                                                                                                               <  1:904620/139‑1 (MQ=255)
                     tttCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATctg                                                                                                                >  2:2017174/1‑139 (MQ=255)
                        cTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCGGTGATcttctg                                                                                                             >  2:1335513/1‑139 (MQ=255)
                         tAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTgg                                                                                                            >  2:1104026/1‑139 (MQ=255)
                           aCCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGc                                                                                                          <  1:1104026/139‑1 (MQ=255)
                           aCCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGc                                                                                                          >  1:211015/1‑139 (MQ=255)
                                    tgGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAgccagcca                                                                                                 <  1:444766/139‑1 (MQ=255)
                                     ggTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCaa                                                                                                >  2:842870/1‑139 (MQ=255)
                                      gTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGc                                                                                                          >  2:1971398/1‑128 (MQ=255)
                                      gTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGc                                                                                                          <  1:1971398/128‑1 (MQ=255)
                                      gTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAAc                                                                                               >  2:1450038/1‑139 (MQ=255)
                                            aTCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTc                                                                                                                               >  1:738335/1‑101 (MQ=255)
                                            aTCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTc                                                                                                                               <  2:738335/101‑1 (MQ=255)
                                            aTCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                         <  2:211015/139‑1 (MQ=255)
                                            aTCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                         <  2:1758585/139‑1 (MQ=255)
                                                ccACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTc                                                                                                                               >  2:1318600/1‑97 (MQ=255)
                                                ccACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTc                                                                                                                               <  1:1318600/97‑1 (MQ=255)
                                                ccACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCaa                                                                                                                             <  2:876639/99‑1 (MQ=255)
                                                ccACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCaa                                                                                                                             >  1:876639/1‑99 (MQ=255)
                                                 cACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCaa                                                                                                                             >  1:47029/1‑98 (MQ=255)
                                                 cACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCaa                                                                                                                             <  2:47029/98‑1 (MQ=255)
                                                 cACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGcttc                                                                                    >  1:1514966/1‑139 (MQ=255)
                                                   cccTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGcttctt                                                                                  >  2:1445911/1‑139 (MQ=255)
                                                        gTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTa                                                                                                                                     <  2:178446/83‑1 (MQ=255)
                                                        gTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTa                                                                                                                                     >  1:178446/1‑83 (MQ=255)
                                                        gTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCaa                                                                                                                             <  1:800539/91‑1 (MQ=255)
                                                        gTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCaa                                                                                                                             >  2:800539/1‑91 (MQ=255)
                                                             cGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCa                                                                        >  2:129062/1‑139 (MQ=255)
                                                                  tttttGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAAc                                                                                                                          >  1:138657/1‑84 (MQ=255)
                                                                  tttttGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAAc                                                                                                                          <  2:138657/84‑1 (MQ=255)
                                                                   ttttGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTttcc                                                                  >  2:1031197/1‑139 (MQ=255)
                                                                     ttGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTttcctt                                                                <  1:842870/139‑1 (MQ=255)
                                                                     ttGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTttcctt                                                                >  2:1791500/1‑139 (MQ=255)
                                                                     ttGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTttcctt                                                                <  1:1445911/139‑1 (MQ=255)
                                                                             ttACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTgcg                                                                          <  1:728504/121‑1 (MQ=255)
                                                                             ttACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTgcg                                                                          >  2:728504/1‑121 (MQ=255)
                                                                               aCTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCa                                                         >  1:577338/1‑136 (MQ=255)
                                                                               aCTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCa                                                         <  2:577338/136‑1 (MQ=255)
                                                                                     gcTACTTCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGt                                                <  1:798738/139‑1 (MQ=255)
                                                                                     gcTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGt                                                >  1:816860/1‑139 (MQ=255)
                                                                                         cGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTc                                                                                                                               <  2:174225/56‑1 (MQ=255)
                                                                                         cGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTc                                                                                                                               >  1:174225/1‑56 (MQ=255)
                                                                                         cGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                         >  1:1590932/1‑94 (MQ=255)
                                                                                         cGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                         <  2:1590932/94‑1 (MQ=255)
                                                                                           ccTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTggtg                                          <  1:2017174/139‑3 (MQ=255)
                                                                                           ccTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGgcg                                          <  1:1450038/139‑1 (MQ=255)
                                                                                           ccTTAAGCTAGGATTACCGGTGTTTGACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTTATCTGCTAATCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCGGgcg                                          <  1:1883603/139‑1 (MQ=255)
                                                                                               aaGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCAt                                                            >  2:867150/1‑117 (MQ=255)
                                                                                               aaGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCAt                                                            <  1:867150/117‑1 (MQ=255)
                                                                                               aaGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTc                                      >  2:1265875/1‑139 (MQ=255)
                                                                                                aGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTcc                                     <  2:1514966/139‑1 (MQ=255)
                                                                                                      gATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATcc                               >  2:2012319/1‑139 (MQ=255)
                                                                                                          aCCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                           <  1:1898397/75‑1 (MQ=255)
                                                                                                          aCCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                           >  2:1898397/1‑75 (MQ=255)
                                                                                                           ccGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCaa                                                                                                                             >  2:1723554/1‑40 (MQ=255)
                                                                                                           ccGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCaa                                                                                                                             <  1:1723554/40‑1 (MQ=255)
                                                                                                           ccGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGgcg                                          <  1:198954/123‑1 (MQ=255)
                                                                                                           ccGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGgcg                                          >  2:198954/1‑123 (MQ=255)
                                                                                                           ccGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTcc                          >  1:393416/1‑139 (MQ=255)
                                                                                                               tCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                         >  2:1324648/1‑72 (MQ=255)
                                                                                                               tCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                         <  1:1324648/72‑1 (MQ=255)
                                                                                                                      ctacAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATc                                <  2:1157571/122‑1 (MQ=255)
                                                                                                                      ctacAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATc                                >  1:1157571/1‑122 (MQ=255)
                                                                                                                         cAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCtgctgc            <  1:2012319/139‑1 (MQ=255)
                                                                                                                         cAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCCTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCtgctgc            >  2:1724281/1‑139 (MQ=255)
                                                                                                                         aaatcACGAAGGAAATTAAGGTTAAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTTCGCATTTTCCTTCCAATCAATAAACTGTCTGACACGTCCCGATCCAGTCCCCGGCAgctgctcc            >  1:984873/5‑137 (MQ=255)
                                                                                                                           gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAAc                                                                                               >  1:1203168/1‑54 (MQ=255)
                                                                                                                           gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAAc                                                                                               <  2:1203168/54‑1 (MQ=255)
                                                                                                                           gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                           >  2:46673/1‑58 (MQ=255)
                                                                                                                           gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                           <  2:1166808/58‑1 (MQ=255)
                                                                                                                           gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                           >  2:1277321/1‑58 (MQ=255)
                                                                                                                           gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                           >  1:1166808/1‑58 (MQ=255)
                                                                                                                           gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                           <  1:46673/58‑1 (MQ=255)
                                                                                                                           gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                           <  1:1277321/58‑1 (MQ=255)
                                                                                                                           gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                         >  1:1296489/1‑60 (MQ=255)
                                                                                                                           gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                         <  2:1296489/60‑1 (MQ=255)
                                                                                                                           gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCa          >  1:508246/1‑139 (MQ=255)
                                                                                                                            ccACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAg         >  1:1598824/1‑139 (MQ=255)
                                                                                                                               cGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAAc      >  2:611140/1‑139 (MQ=255)
                                                                                                                                 aaGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAACTg    >  1:604806/1‑139 (MQ=255)
                                                                                                                                   ggACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAACTGCt  <  1:129062/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                     
ACATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAACTGCT  >  NC_000913/3819352‑3819621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑