Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,842,455	C→T	*445* (TAG→TAA)	adeQ ←	adenine permease, high affinity, adenine:H+ symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,842,455	0	C	T	100.0%	277.8 / NA	85	*445* (TAG→TAA)	adeQ	adenine permease, high affinity, adenine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/42);  total (43/42)

ATCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCG  >  NC_000913/3842320‑3842584                                                                                                                                        |                                                                                                                                  aTCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTaa                                                                                                                                <  1:1750690/139‑1 (MQ=255) aTCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTaa                                                                                                                                <  2:445395/139‑1 (MQ=255)   cGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATc                                                                                                                              <  1:1111315/139‑1 (MQ=255)             gCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGaata                                                                                                                    >  1:1330743/1‑139 (MQ=255)                  ggATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCtt                                                                                                               <  2:2051753/139‑1 (MQ=255)                   gATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTc                                                                                                              <  2:549143/139‑1 (MQ=255)                      gTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGt                                                                                                           <  2:1330743/139‑1 (MQ=255)                              ccGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACag                                                                                                   >  2:207958/1‑139 (MQ=255)                                 aaaaCCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGc                                                                                                >  1:1124946/1‑139 (MQ=255)                                         ccaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                        <  1:207958/139‑1 (MQ=255)                                                   tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                              <  2:477330/139‑1 (MQ=255)                                                   tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                              <  2:1124946/139‑1 (MQ=255)                                                   tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                              <  1:388363/139‑1 (MQ=255)                                                    aCCGCCAGCGTGGCGCGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                              <  1:530793/138‑1 (MQ=255)                                                    aCCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATc                                                                                                                              >  2:276997/1‑90 (MQ=255)                                                    aCCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATc                                                                                                                              <  1:276997/90‑1 (MQ=255)                                                    aCCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacac                                                                             >  1:559402/1‑139 (MQ=255)                                                    aCCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacac                                                                             >  1:186561/1‑139 (MQ=255)                                                    aCCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                              >  1:751896/1‑138 (MQ=255)                                                    aCCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                              <  2:751896/138‑1 (MQ=255)                                                    aCCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                              >  2:530793/1‑138 (MQ=255)                                                       gCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATc                                                                                                                              >  1:474012/1‑87 (MQ=255)                                                       gCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATc                                                                                                                              <  2:474012/87‑1 (MQ=255)                                                        ccAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGt                                                                         >  2:468788/1‑139 (MQ=255)                                                          aGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTc                                                                       >  2:1966038/1‑139 (MQ=255)                                                           gCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCa                                                                      >  1:314370/1‑139 (MQ=255)                                                               ggCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccac                                                                                       <  2:1307398/118‑1 (MQ=255)                                                               ggCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccac                                                                                       >  1:1307398/1‑118 (MQ=255)                                                                     cATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCa                                                            <  2:314370/139‑1 (MQ=255)                                                                          tCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                       <  2:559402/139‑1 (MQ=255)                                                                                  aTGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCtt                                                                                                               >  2:1981911/1‑75 (MQ=255)                                                                                  aTGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCtt                                                                                                               <  1:1981911/75‑1 (MQ=255)                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGAccaccacca                                                                                  >  2:604374/1‑102 (MQ=255)                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGAccaccacca                                                                                  <  1:604374/102‑1 (MQ=255)                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                              >  1:1207155/1‑106 (MQ=255)                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                              <  2:1207155/106‑1 (MQ=255)                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc                                             >  1:244532/1‑139 (MQ=255)                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                              >  2:39077/1‑101 (MQ=255)                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                              <  1:39077/101‑1 (MQ=255)                                                                                           ccTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatg                                      >  1:1630212/1‑139 (MQ=255)                                                                                           ccTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatg                                      <  2:244532/139‑1 (MQ=255)                                                                                               cGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGc                                  >  2:1831390/1‑139 (MQ=255)                                                                                                gTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcaca                                                                                <  2:781925/92‑1 (MQ=255)                                                                                                gTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcaca                                                                                >  1:781925/1‑92 (MQ=255)                                                                                                 tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGa                                                                                                                       >  1:1401067/1‑52 (MQ=255)                                                                                                 tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGa                                                                                                                       <  2:1401067/52‑1 (MQ=255)                                                                                                 tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCa                                                <  1:868769/123‑1 (MQ=255)                                                                                                 tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCa                                                >  2:868769/1‑123 (MQ=255)                                                                                                   aTCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTa                              <  2:222972/139‑1 (MQ=255)                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATc                                                                                                                              >  1:2020860/1‑42 (MQ=255)                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATc                                                                                                                              <  2:2020860/42‑1 (MQ=255)                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCt                                                                                                                <  1:741621/56‑1 (MQ=255)                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCt                                                                                                                >  2:741621/1‑56 (MQ=255)                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTAc                             >  1:914520/1‑139 (MQ=255)                                                                                                       gAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGAc                          >  2:544842/1‑139 (MQ=255)                                                                                                        aaCTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc                                             <  1:1872546/119‑1 (MQ=255)                                                                                                        aaCTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc                                             >  2:1872546/1‑119 (MQ=255)                                                                                                             aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGt                                                                                                           <  2:1636241/52‑1 (MQ=255)                                                                                                             aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGt                                                                                                           >  2:1551142/1‑52 (MQ=255)                                                                                                             aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGt                                                                                                           >  1:1636241/1‑52 (MQ=255)                                                                                                             aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGt                                                                                                           <  1:1551142/52‑1 (MQ=255)                                                                                                             aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc                                             <  2:607918/114‑1 (MQ=255)                                                                                                             aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc                                             >  1:607918/1‑114 (MQ=255)                                                                                                               gATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGcc                  <  1:468788/139‑1 (MQ=255)                                                                                                                  atCTTGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGccaccacca                                                                                     >  1:2055565/1‑69 (MQ=255)                                                                                                                  atCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccacca                                                                                     <  2:2055565/69‑1 (MQ=255)                                                                                                                     tGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCaaa                                                                                                      >  2:828123/1‑49 (MQ=255)                                                                                                                     tGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCaaa                                                                                                      <  1:828123/49‑1 (MQ=255)                                                                                                                     tGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAg                                                           <  2:718214/92‑1 (MQ=255)                                                                                                                     tGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAg                                                           >  1:718214/1‑92 (MQ=255)                                                                                                                     tGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGc            <  2:1630212/139‑1 (MQ=255)                                                                                                                      ggAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                              <  2:1238473/72‑1 (MQ=255)                                                                                                                      ggAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                              >  1:1238473/1‑72 (MQ=255)                                                                                                                      ggAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCa           >  2:769247/1‑139 (MQ=255)                                                                                                                       gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                          <  2:1655693/59‑1 (MQ=255)                                                                                                                       gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                          >  1:1655693/1‑59 (MQ=255)                                                                                                                             cTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGAccaccacca                                                                                  <  1:929768/61‑1 (MQ=255)                                                                                                                             cTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGAccaccacca                                                                                  >  2:929768/1‑61 (MQ=255)                                                                                                                             cTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                              <  1:977281/65‑1 (MQ=255)                                                                                                                             cTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                              >  2:977281/1‑65 (MQ=255)                                                                                                                             cTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCtt    >  2:707866/1‑139 (MQ=255)                                                                                                                             cTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCtt    >  1:345511/1‑139 (MQ=255)                                                                                                                              tCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGAccaccacca                                                                                  <  1:767617/60‑1 (MQ=255)                                                                                                                              tCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGAccaccacca                                                                                  >  2:767617/1‑60 (MQ=255)                                                                                                                               cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCg  >  2:1493723/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                  ATCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCG  >  NC_000913/3842320‑3842584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑