Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	344,991	G→A	*272* (TAG→TAA)	yahL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	344,991	0	G	A	100.0%	231.7 / NA	72	*272* (TAG→TAA)	yahL	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/33);  total (39/33)

TGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGG  >  NC_000913/344858‑345125                                                                                                                                      |                                                                                                                                       tGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAg                                                                                                                                   >  1:1079659/1‑139 (MQ=255)     ctctGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAg                                                                                                                               >  2:1591168/1‑139 (MQ=255)         gTTCTGGACGT‑AAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTc                                                                                                                          <  2:474307/139‑1 (MQ=255)                            aCAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                        <  2:1706516/139‑1 (MQ=255)                            aCAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                        >  1:1706516/1‑139 (MQ=255)                                     atgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgag                                                                                               >  2:1861284/1‑139 (MQ=255)                                     atgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGATACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgag                                                                                               >  2:1733150/1‑139 (MQ=255)                                      tgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgaga                                                                                              <  2:1079659/139‑1 (MQ=255)                                        cgcgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCTCCTGAGAGt                                                                                            >  2:1317401/1‑139 (MQ=255)                                         gcgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           <  1:1733150/139‑1 (MQ=255)                                          cgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGa                                                                                          >  2:1477826/1‑139 (MQ=255)                                              tAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCg                                                                                      <  1:1317401/139‑1 (MQ=255)                                                 atgaatgGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCaa                                                                                   <  1:1477826/139‑1 (MQ=255)                                                                    agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatc                                                                <  1:1591168/139‑1 (MQ=255)                                                                      tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTTTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCa                                                                                    <  1:2028968/117‑1 (MQ=255)                                                                      tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTTTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCa                                                                                    >  2:2028968/1‑117 (MQ=255)                                                                      tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                                                 >  2:1948474/1‑72 (MQ=255)                                                                      tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGaa                                                                                                                                 <  1:1948474/72‑1 (MQ=255)                                                                      tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTa                                                                                                          <  1:1998175/95‑1 (MQ=255)                                                                      tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTa                                                                                                          >  2:1998175/1‑95 (MQ=255)                                                                      tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAc                                                                                                    >  1:1975553/1‑101 (MQ=255)                                                                      tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAc                                                                                                    <  2:1975553/101‑1 (MQ=255)                                                                      tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 >  2:625573/1‑136 (MQ=255)                                                                      tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 <  1:625573/136‑1 (MQ=255)                                                                      tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 >  1:509227/1‑136 (MQ=255)                                                                      tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 <  2:509227/136‑1 (MQ=255)                                                                       gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   >  2:1528808/1‑133 (MQ=255)                                                                       gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   <  1:1528808/133‑1 (MQ=255)                                                                       gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgt                                                             >  2:620093/1‑139 (MQ=255)                                                                        cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  <  2:1038021/85‑1 (MQ=255)                                                                        cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  >  1:1038021/1‑85 (MQ=255)                                                                        cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           <  1:864002/108‑1 (MQ=255)                                                                        cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           >  2:864002/1‑108 (MQ=255)                                                                        cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                            >  2:69818/1‑139 (MQ=255)                                                                                aGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  >  2:1532489/1‑77 (MQ=255)                                                                                aGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  <  1:1532489/77‑1 (MQ=255)                                                                                aGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGt                                                                            <  2:1941193/115‑1 (MQ=255)                                                                                aGGTGACAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGt                                                                            >  1:1941193/1‑115 (MQ=255)                                                                                     aCAGATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCa                                               >  2:205225/1‑139 (MQ=255)                                                                                        gATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgaga                                                                                              >  1:1050602/1‑89 (MQ=255)                                                                                        gATATTATCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgaga                                                                                              <  2:1050602/89‑1 (MQ=255)                                                                                               tCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   >  2:1408429/1‑109 (MQ=255)                                                                                               tCCTGTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   <  1:1408429/109‑1 (MQ=255)                                                                                                   gTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                        >  2:599266/1‑68 (MQ=255)                                                                                                   gTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                        <  1:599266/68‑1 (MQ=255)                                                                                                   gTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                                           <  2:897338/113‑1 (MQ=255)                                                                                                   gTAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                                           >  1:897338/1‑113 (MQ=255)                                                                                                    tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           >  2:972837/1‑80 (MQ=255)                                                                                                    tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           <  1:2027304/80‑1 (MQ=255)                                                                                                    tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           >  2:2027304/1‑80 (MQ=255)                                                                                                    tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           <  1:972837/80‑1 (MQ=255)                                                                                                    tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 >  2:1579009/1‑106 (MQ=255)                                                                                                    tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 <  1:1579009/106‑1 (MQ=255)                                                                                                    tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGcc                                                >  1:935012/1‑123 (MQ=255)                                                                                                    tAAATAAGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGcc                                                <  2:935012/123‑1 (MQ=255)                                                                                                         aaGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGc                                                                                     >  1:1546770/1‑81 (MQ=255)                                                                                                         aaGCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGc                                                                                     <  2:1546770/81‑1 (MQ=255)                                                                                                           gCATGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCtttttt                         <  1:205225/139‑1 (MQ=255)                                                                                                            catgTTAAAATCCGCTACACTCTTTAATTCCGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAAt                                                                                        <  1:1452842/72‑1 (MQ=255)                                                                                                            catgTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAAt                                                                                        >  2:1452842/4‑75 (MQ=255)                                                                                                              tgTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGc                      >  2:882403/2‑139 (MQ=255)                                                                                                                        cGCTACACTCTGTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAAc                                             <  2:867747/106‑1 (MQ=255)                                                                                                                        cGCTACACTCATTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAAc                                             >  1:867747/1‑106 (MQ=255)                                                                                                                          cTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                                           <  2:2042743/90‑1 (MQ=255)                                                                                                                          cTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                                           >  1:2042743/1‑90 (MQ=255)                                                                                                                                tctTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCACTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATc    >  1:1619726/1‑139 (MQ=255)                                                                                                                                  tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   >  2:1357055/1‑74 (MQ=255)                                                                                                                                  tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATg                                                  >  1:943979/1‑91 (MQ=255)                                                                                                                                  tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATg                                                  <  2:943979/91‑1 (MQ=255)                                                                                                                                  tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCgg  >  2:921668/1‑139 (MQ=255)                                                                                                                                  tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCgg  >  1:215911/1‑139 (MQ=255)                                                                                                                                  tttAATTCAGAAAGCGTTCCACCTGCAAGTTGTTGTTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   <  1:1357055/74‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       TGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGG  >  NC_000913/344858‑345125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑