Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,897,439	G→A	*222* (TAG→TAA)	yieH →	phosphoenolpyruvate and 6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,897,439	0	G	A	100.0%	304.0 / NA	93	*222* (TAG→TAA)	yieH	phosphoenolpyruvate and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/48);  total (45/48)

GTATGGAAGTGTTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAGTTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTT  >  NC_000913/3897309‑3897564                                                                                                                                   |                                                                                                                              gTATGGAAGTGTTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcac                                                                                                                       <  2:1183078/139‑1 (MQ=255)        aGTGTTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTCTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCt                                                                                                                <  1:1471966/139‑1 (MQ=255)           gtTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCa                                                                                                             >  2:1981545/1‑139 (MQ=255)           gtTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCa                                                                                                             >  2:372694/1‑139 (MQ=255)           gtTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCa                                                                                                             >  2:795871/1‑139 (MQ=255)           gtTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCa                                                                                                             >  2:629630/1‑139 (MQ=255)            ttCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAAttc                                                                                                                            <  1:1382673/123‑1 (MQ=255)                      gcgcCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcac                                                                                                                       <  1:827786/118‑1 (MQ=255)                      gcgcCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcac                                                                                                                       >  2:827786/1‑118 (MQ=255)                            aCCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAAtt                                                                                            >  1:324020/1‑139 (MQ=255)                             cccGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTg                                                                                           <  2:324020/139‑1 (MQ=255)                                        aGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                <  1:1981545/139‑1 (MQ=255)                                           cGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGgtaa                                                                             >  1:152924/1‑139 (MQ=255)                                            gATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcaca                                                                                                                      <  2:864010/97‑1 (MQ=255)                                            gATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcaca                                                                                                                      >  1:864010/1‑97 (MQ=255)                                            gATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGgtaag                                                                            >  2:304344/1‑139 (MQ=255)                                              tCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTccc                                                                                                                 >  1:1818840/1‑100 (MQ=255)                                              tCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                >  1:1959951/1‑133 (MQ=255)                                              tCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                <  2:1959951/133‑1 (MQ=255)                                              tCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGgtaagta                                                                          >  2:1674292/1‑139 (MQ=255)                                              tCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTAGTTGGGATATTACGGCATAATTCTTCACACTccc                                                                                                                 <  2:1818840/100‑1 (MQ=255)                                                gTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                <  2:709756/131‑1 (MQ=255)                                                gTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                >  1:709756/1‑131 (MQ=255)                                                gTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTaaa                                                                        <  1:629630/139‑1 (MQ=255)                                                gTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTaaa                                                                        <  1:304344/139‑1 (MQ=255)                                                 ttCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAg                                                                       <  1:372694/139‑1 (MQ=255)                                                      ccGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGt                                                                  <  2:667099/139‑1 (MQ=255)                                                           aGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGat                                                             <  1:795871/139‑1 (MQ=255)                                                                       tACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCacac                                                                                                                     >  2:1118993/1‑71 (MQ=255)                                                                       tACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCacac                                                                                                                     <  1:1118993/71‑1 (MQ=255)                                                                         cccATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACg                                               <  1:1488171/139‑1 (MQ=255)                                                                           cATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTc                                             <  1:549873/139‑1 (MQ=255)                                                                           cATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTc                                             <  2:865995/139‑1 (MQ=255)                                                                           cATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTc                                             <  2:994212/139‑1 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAAttc                                                                                                                            >  1:1559904/1‑57 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAAttc                                                                                                                            <  2:1559904/57‑1 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcac                                                                                                                       <  1:258224/62‑1 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcac                                                                                                                       >  2:258224/1‑62 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcac                                                                                                                       <  1:599466/62‑1 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcac                                                                                                                       >  2:599466/1‑62 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCacac                                                                                                                     <  2:945842/64‑1 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCacac                                                                                                                     >  1:945842/1‑64 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCt                                                                                      >  1:123765/1‑95 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCt                                                                                      <  2:123765/95‑1 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAg                                                                   >  2:1116871/1‑114 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAg                                                                   <  1:1116871/114‑1 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGtt                                                                 <  1:1542146/116‑1 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGtt                                                                 >  2:1542146/1‑116 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGatat                                                           >  1:1212197/1‑122 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGatat                                                           <  2:1212197/122‑1 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGt                                                         <  2:885024/124‑1 (MQ=255)                                                                              cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGt                                                         >  1:885024/1‑124 (MQ=255)                                                                                 tCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTaa                                       >  2:33987/1‑139 (MQ=255)                                                                                         aCCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcac                                                                                                                       <  2:791671/51‑1 (MQ=255)                                                                                         aCCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcac                                                                                                                       >  1:791671/1‑51 (MQ=255)                                                                                                   tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcaca                                                                                                                      >  2:1226315/1‑42 (MQ=255)                                                                                                   tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCa                                                                                    >  1:1002603/1‑76 (MQ=255)                                                                                                   tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCa                                                                                    <  1:1857075/76‑1 (MQ=255)                                                                                                   tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCa                                                                                    >  2:1857075/1‑76 (MQ=255)                                                                                                   tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCa                                                                                    <  2:1002603/76‑1 (MQ=255)                                                                                                   tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                >  2:397622/1‑80 (MQ=255)                                                                                                   tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                <  1:397622/80‑1 (MQ=255)                                                                                                   tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAg                                                                       <  2:1331014/89‑1 (MQ=255)                                                                                                   tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGt                                              >  2:1697026/1‑114 (MQ=255)                                                                                                   tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGt                                              <  1:1697026/114‑1 (MQ=255)                                                                                                   tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTAtt                     >  2:485056/1‑139 (MQ=255)                                                                                                   tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTAtt                     >  2:1007654/1‑139 (MQ=255)                                                                                                   tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTAtt                     >  2:497205/1‑139 (MQ=255)                                                                                                   tgGAAAGCGCGTGGTTGGGATATTACGGAATAATTCTTCACACTCCCTTTACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAg                                                                       >  1:1331014/1‑89 (MQ=255)                                                                                                   tgGAAAGCGCGTGGGTGGGATATTACGGCATAATTCTTcaca                                                                                                                      <  1:1226315/42‑1 (MQ=255)                                                                                                    ggAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTccc                                                                                                                 >  2:127201/1‑46 (MQ=255)                                                                                                    ggAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTccc                                                                                                                 <  1:127201/46‑1 (MQ=255)                                                                                                     gAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTc                                             <  2:1551607/113‑1 (MQ=255)                                                                                                     gAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTc                                             >  1:1551607/1‑113 (MQ=255)                                                                                                     gAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTAtttt                   >  2:246622/1‑139 (MQ=255)                                                                                                          cgcgTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                <  1:1272118/73‑1 (MQ=255)                                                                                                          cgcgTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                >  2:1272118/1‑73 (MQ=255)                                                                                                             gTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCa                                                                                    >  2:897457/1‑66 (MQ=255)                                                                                                             gTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCa                                                                                    <  1:897457/66‑1 (MQ=255)                                                                                                             gTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                <  2:1258444/70‑1 (MQ=255)                                                                                                             gTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                >  1:1258444/1‑70 (MQ=255)                                                                                                             gTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGgtaagtaa                                                                         <  2:60571/77‑1 (MQ=255)                                                                                                             gTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGgtaagtaa                                                                         >  1:60571/1‑77 (MQ=255)                                                                                                               ggTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAg         <  2:152924/139‑1 (MQ=255)                                                                                                                   gggATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGa       >  2:1255378/1‑137 (MQ=255)                                                                                                                   gggATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGa       <  1:1255378/137‑1 (MQ=255)                                                                                                                      atatTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGtt  <  1:1674292/139‑1 (MQ=255)                                                                                                                         ttACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTg                  <  1:534714/120‑1 (MQ=255)                                                                                                                         ttACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTg                  >  2:534714/1‑120 (MQ=255)                                                                                                                           aCGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACg                                               >  1:1701282/1‑89 (MQ=255)                                                                                                                           aCGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACg                                               <  2:1701282/89‑1 (MQ=255)                                                                                                                               cATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGat                                                             >  1:1244511/1‑71 (MQ=255)                                                                                                                               cATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGat                                                             <  2:1244511/71‑1 (MQ=255)                                                                                                                                   |                                                                                                                              GTATGGAAGTGTTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAGTTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTT  >  NC_000913/3897309‑3897564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑