Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,898,609	G→A	*196* (TAG→TAA)	cbrC →	UPF0167 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,898,609	0	G	A	100.0%	185.9 / NA	58	*196* (TAG→TAA)	cbrC	UPF0167 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/31);  total (27/31)

GAAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGG  >  NC_000913/3898478‑3898744                                                                                                                                    |                                                                                                                                        gaagaTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTa                                                                                                                                  <  2:213026/139‑1 (MQ=255)  aagaTTGTGATAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                                 <  1:1859784/139‑1 (MQ=255)    gaTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAaat                                                                                                                               >  2:1868247/1‑139 (MQ=255)       tgtgAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAaataat                                                                                                                            >  1:827605/1‑139 (MQ=255)         tgAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                                 <  2:1601988/132‑1 (MQ=255)         tgAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                                 >  1:1601988/1‑132 (MQ=255)         tgAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATga                                                                                                                          >  1:1289877/1‑139 (MQ=255)                 tCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGc                                                                                                                  >  2:179401/1‑139 (MQ=255)                   ggTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCg                                                                                                                <  2:1289877/139‑1 (MQ=255)                               tCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTaa                                                                                                        >  1:1328607/1‑135 (MQ=255)                               tCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTaa                                                                                                        <  2:1328607/135‑1 (MQ=255)                               tCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                                                    <  2:827605/139‑1 (MQ=255)                                cTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTcac                                                                                                   <  2:1164924/139‑1 (MQ=255)                                        gCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                                 <  1:1379129/101‑1 (MQ=255)                                        gCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                                 >  2:1379129/1‑101 (MQ=255)                                        gCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATgaga                                                                                                                        <  1:1873693/110‑1 (MQ=255)                                        gCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATgaga                                                                                                                        >  2:1873693/1‑110 (MQ=255)                                         gcatttagcTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCg                                                                                                                <  1:1851827/109‑1 (MQ=255)                                         gcatttagcTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCg                                                                                                                >  2:1851827/9‑117 (MQ=255)                                           aaaTGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTaa                                                                                                        >  2:332363/1‑123 (MQ=255)                                           aaaTGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCCCTGGCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTaa                                                                                                        <  1:332363/123‑1 (MQ=255)                                                  tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTa                                                                                                         >  2:1255147/1‑115 (MQ=255)                                                  tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTa                                                                                                         <  1:1255147/115‑1 (MQ=255)                                                  tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCg                                                                                            <  1:435968/128‑1 (MQ=255)                                                  tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCg                                                                                            >  2:435968/1‑128 (MQ=255)                                                      aaaGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACAt                                                                                               <  1:496974/121‑1 (MQ=255)                                                      aaaGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACAt                                                                                               >  2:496974/1‑121 (MQ=255)                                                              gtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                                 >  2:1903580/1‑79 (MQ=255)                                                              gtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                                 <  1:1903580/79‑1 (MQ=255)                                                              gtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                                     <  1:261860/139‑1 (MQ=255)                                                              gtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                                     >  2:1666986/1‑139 (MQ=255)                                                              gtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                                     <  2:1408473/139‑1 (MQ=255)                                                                    gTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTat                                                                                                                                 <  2:1264581/73‑2 (MQ=255)                                                                    gTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                                 >  1:1264581/1‑73 (MQ=255)                                                                         ggTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATc                                                                                              <  1:1899655/103‑1 (MQ=255)                                                                         ggTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATc                                                                                              >  2:1899655/1‑103 (MQ=255)                                                                             aTCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTc                                                                                  <  2:733221/111‑1 (MQ=255)                                                                             aTCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTc                                                                                  >  1:733221/1‑111 (MQ=255)                                                                                tctTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCa                                                   <  1:1666986/139‑1 (MQ=255)                                                                                   tCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTa                                                                                                                                  >  2:1377698/1‑57 (MQ=255)                                                                                   tCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCATAATTATTTa                                                                                                                                  <  1:1377698/57‑1 (MQ=255)                                                                                     cGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGc                                              >  2:1185010/1‑139 (MQ=255)                                                                                          tctcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTAt                                                                                                                                     >  2:81055/1‑47 (MQ=255)                                                                                          tctcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTAt                                                                                                                                     <  1:81055/47‑1 (MQ=255)                                                                                                 tGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCtt                                                                                <  1:765519/93‑1 (MQ=255)                                                                                                 tGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCtt                                                                                >  2:765519/1‑93 (MQ=255)                                                                                                  gCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAtt                                 <  1:1664738/139‑1 (MQ=255)                                                                                                    ggCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                                 >  1:130198/1‑41 (MQ=255)                                                                                                    ggCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                                 <  2:130198/41‑1 (MQ=255)                                                                                                    ggCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTaa                                                                                                        <  1:45631/66‑1 (MQ=255)                                                                                                    ggCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTaa                                                                                                        >  2:45631/1‑66 (MQ=255)                                                                                                    ggCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAc                               >  2:714924/1‑139 (MQ=255)                                                                                                    ggCAAGCTGAGACTGTGGGGTGA‑TTTTCGTAATTATTTAAATAATGAGAACAGGCCg                                                                                                                <  2:1040099/57‑1 (MQ=255)                                                                                                    ggCAAGCTGAGACTGTGGGGTGA‑TTTTCGTAATTATTTAAATAATGAGAACAGGCCg                                                                                                                >  1:1040099/1‑57 (MQ=255)                                                                                                            gagaCTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCg                       <  2:113389/139‑1 (MQ=255)                                                                                                                    ggggTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGc               >  1:59266/1‑139 (MQ=255)                                                                                                                                cGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACg   <  1:1379465/139‑1 (MQ=255)                                                                                                                                 gTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGGGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACgg  <  1:1185010/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        GAAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGG  >  NC_000913/3898478‑3898744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑