Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	345,649	G→A	*82* (TAG→TAA)	yahM →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	345,649	0	G	A	100.0%	247.0 / NA	75	*82* (TAG→TAA)	yahM	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/38);  total (37/38)

CACAGGGGAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAGAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATACGCCCATAAGCACGACGCACA  >  NC_000913/345523‑345774                                                                                                                               |                                                                                                                               cacaGGGGAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTAt                                                                                                                    <  2:50195/139‑1 (MQ=255)      gggAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCt                                                                                                               <  2:733803/139‑1 (MQ=255)      gggAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCt                                                                                                               <  2:1429789/139‑1 (MQ=255)            gTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGc                                                                                                         <  2:1586877/139‑1 (MQ=255)            gTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGc                                                                                                         >  2:469443/1‑139 (MQ=255)              gggCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTg                                                                                                       >  2:1915445/1‑139 (MQ=255)                gCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCTGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCg                                                                                                     >  1:1725560/1‑139 (MQ=255)                gCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTAt                                                                                                                    >  2:1637922/1‑124 (MQ=255)                gCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTAt                                                                                                                    <  1:1637922/124‑1 (MQ=255)                gCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCg                                                                                                     >  2:466505/1‑139 (MQ=255)                  tGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTc                                                                                                   >  2:1972463/1‑139 (MQ=255)                     aaTGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCAcc                                                                                                <  1:469443/139‑1 (MQ=255)                      aTGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCAccc                                                                                               <  1:1972463/139‑1 (MQ=255)                                aaaGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAAt                                                                                     <  2:1725560/139‑1 (MQ=255)                                      tCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGc                                                                                                         <  1:1661112/113‑1 (MQ=255)                                      tCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGc                                                                                                         >  2:1661112/1‑113 (MQ=255)                                        aaGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGTTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCAccc                                                                                               >  1:1322266/1‑121 (MQ=255)                                        aaGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCAccc                                                                                               <  2:1322266/121‑1 (MQ=255)                                        aaGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGt                                                                              <  1:1362173/138‑1 (MQ=255)                                        aaGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGt                                                                              >  2:1362173/1‑138 (MQ=255)                                                ttATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAAt                                                                    <  2:25292/139‑1 (MQ=255)                                                  aTCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGt                                                                              >  1:154748/1‑128 (MQ=255)                                                  aTCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGt                                                                              <  2:154748/128‑1 (MQ=255)                                                   tCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCg                                                                                                          >  2:1217972/1‑99 (MQ=255)                                                   tCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCg                                                                                                          <  1:1217972/99‑1 (MQ=255)                                                         aTAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCaata                                                           <  2:58766/139‑1 (MQ=255)                                                          tAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑Ga                                                                      <  1:1498441/127‑1 (MQ=255)                                                          tAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑Ga                                                                      >  2:1498441/1‑127 (MQ=255)                                                                 cACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑Gaa                                                                     >  1:871766/1‑121 (MQ=255)                                                                 cACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑Gaa                                                                     <  2:871766/121‑1 (MQ=255)                                                                 cACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAAt                                                                    >  2:485463/1‑122 (MQ=255)                                                                 cACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAAt                                                                    <  1:485463/122‑1 (MQ=255)                                                                   cGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATa                                                                   <  1:1504475/121‑1 (MQ=255)                                                                   cGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATa                                                                   >  2:1504475/1‑121 (MQ=255)                                                                   cGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCaa                                                 >  1:1580534/1‑139 (MQ=255)                                                                   cGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCaa                                                 >  1:83778/1‑139 (MQ=255)                                                                   cGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCCCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACAcc                                                               >  2:518288/1‑125 (MQ=255)                                                                   cGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCCCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACAcc                                                               <  1:518288/125‑1 (MQ=255)                                                                              ggCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCg                                                                                                          >  2:1091749/1‑72 (MQ=255)                                                                              ggCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCg                                                                                                          <  1:1091749/72‑1 (MQ=255)                                                                               gCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGt                                                                                                    >  2:128438/1‑77 (MQ=255)                                                                               gCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGt                                                                                                    <  1:128438/77‑1 (MQ=255)                                                                                cTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTAt                                                                                                                    >  2:722499/1‑60 (MQ=255)                                                                                cTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTAt                                                                                                                    <  1:722499/60‑1 (MQ=255)                                                                                cTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAACCCGACTGGc                                    <  1:1915445/139‑1 (MQ=255)                                                                                  atcatcGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATa                                                                   <  2:2045117/106‑1 (MQ=255)                                                                                  atcatcGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATa                                                                   >  1:2045117/1‑106 (MQ=255)                                                                                  atcatcGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAAc                                             >  1:1400527/1‑128 (MQ=255)                                                                                  atcatcGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAAc                                             <  2:1400527/128‑1 (MQ=255)                                                                                  atcatcGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAACCCGACTGGCaa                                  >  2:1242457/1‑139 (MQ=255)                                                                                    catcGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTg                                                       >  2:1456688/1‑116 (MQ=255)                                                                                    catcGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTg                                                       <  1:1456688/116‑1 (MQ=255)                                                                                       cGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGt                             <  1:1132474/139‑1 (MQ=255)                                                                                         ccGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTAt                                                                                                                    <  1:1887155/51‑1 (MQ=255)                                                                                         ccGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTAt                                                                                                                    >  2:1887155/1‑51 (MQ=255)                                                                                         ccGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAgc                                                                           >  2:1282473/1‑92 (MQ=255)                                                                                         ccGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAgc                                                                           <  1:1282473/92‑1 (MQ=255)                                                                                         ccGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGtt                            >  1:1105834/1‑139 (MQ=255)                                                                                         ccGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTg                           >  2:1100383/1‑139 (MQ=255)                                                                                         ccGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTg                           >  1:1076460/1‑139 (MQ=255)                                                                                            aTGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCAt                        <  1:1242457/139‑1 (MQ=255)                                                                                             tGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑Gaa                                                                     >  2:1427175/1‑93 (MQ=255)                                                                                             tGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑Gaa                                                                     <  1:1427175/93‑1 (MQ=255)                                                                                             tGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAAt                                                                    >  1:787883/1‑94 (MQ=255)                                                                                             tGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAAt                                                                    <  2:787883/94‑1 (MQ=255)                                                                                             tGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAAc                                             <  1:1426104/117‑1 (MQ=255)                                                                                             tGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAAc                                             >  2:1426104/1‑117 (MQ=255)                                                                                                        cGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAACCCGACTGGCAACGTGTTGCATACGCCCATAAGc            <  2:1580534/139‑1 (MQ=255)                                                                                                          gCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATACGCCCATAAGCac          >  1:1726067/1‑139 (MQ=255)                                                                                                              aTCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATACGCCCATAAGCacgacg      >  1:995569/1‑139 (MQ=255)                                                                                                                  ggTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATACGCCCATAAGCACGACGcaca  <  2:1076460/139‑1 (MQ=255)                                                                                                                        ttACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATa                       <  2:1356426/112‑1 (MQ=255)                                                                                                                        ttACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATa                       >  1:1356426/1‑112 (MQ=255)                                                                                                                          aCGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAg                                                                            <  2:259495/58‑1 (MQ=255)                                                                                                                          aCGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAg                                                                            >  1:259495/1‑58 (MQ=255)                                                                                                                               |                                                                                                                               CACAGGGGAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAGAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGC‑GAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATACGCCCATAAGCACGACGCACA  >  NC_000913/345523‑345774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑