Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,984,193 G→A *412* (TAG→TAA aslB → putative AslA‑specific sulfatase‑maturating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,984,1930GA100.0% 255.4 / NA 78*412* (TAG→TAAaslBputative AslA‑specific sulfatase‑maturating enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/38);  total (40/38)

TTGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAA  >  NC_000913/3984065‑3984325
                                                                                                                                |                                                                                                                                    
ttGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATcggcgg                                                                                                                            <  2:1918576/139‑1 (MQ=255)
ttGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATcggcgg                                                                                                                            <  1:364162/139‑1 (MQ=255)
         gggTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCg                                                                                                                   >  1:771463/1‑139 (MQ=255)
              tCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGa                                                                                                              <  2:238015/139‑1 (MQ=255)
                       tttCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATcg                                                                                                                                >  1:346278/1‑112 (MQ=255)
                       tttCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATcg                                                                                                                                <  2:346278/112‑1 (MQ=255)
                            gCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATgcg                                                                                                  <  1:137378/137‑1 (MQ=255)
                            gCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATgcg                                                                                                  >  2:137378/1‑137 (MQ=255)
                                          tatCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGAt                                                                                                     >  2:1983432/1‑120 (MQ=255)
                                          tatCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGAt                                                                                                     <  1:1983432/120‑1 (MQ=255)
                                          tatCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATc                                                                                  >  2:244105/1‑139 (MQ=255)
                                          tatCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATc                                                                                  <  1:244105/139‑1 (MQ=255)
                                            tCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                <  2:1236625/139‑1 (MQ=255)
                                            tCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                <  2:771463/139‑1 (MQ=255)
                                                      aTGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGTGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGa                                                                      >  2:1127932/1‑139 (MQ=255)
                                                      aTGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGgcc                                                                                                                          >  1:221213/1‑87 (MQ=255)
                                                      aTGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGgcc                                                                                                                          <  2:221213/87‑1 (MQ=255)
                                                      aTGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                <  1:768362/129‑1 (MQ=255)
                                                      aTGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                >  2:768362/1‑129 (MQ=255)
                                                       tGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                <  2:261728/128‑1 (MQ=255)
                                                       tGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                >  1:261728/1‑128 (MQ=255)
                                                       tGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGAt                                                                     >  2:1975610/1‑139 (MQ=255)
                                                       tGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGAt                                                                     >  1:1175649/1‑139 (MQ=255)
                                                                        cACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATaaa                                                    <  2:1391931/139‑1 (MQ=255)
                                                                         aCGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAAt                                                   >  1:1551435/1‑139 (MQ=255)
                                                                               gCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATgcg                                                                                                  >  2:559012/1‑86 (MQ=255)
                                                                               gCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATgcg                                                                                                  <  1:559012/86‑1 (MQ=255)
                                                                               gCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGc                                             <  1:1975610/139‑1 (MQ=255)
                                                                               gCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGc                                             <  1:697442/139‑1 (MQ=255)
                                                                                cccGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATgc                                                                                                   >  1:155063/1‑84 (MQ=255)
                                                                                cccGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATgc                                                                                                   <  2:155063/84‑1 (MQ=255)
                                                                                     ccAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa                                                                         >  2:459037/1‑105 (MQ=255)
                                                                                     ccAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa                                                                         <  1:459037/105‑1 (MQ=255)
                                                                                     ccAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTg                                       <  2:1175649/139‑1 (MQ=255)
                                                                                       aGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                >  2:1753632/1‑96 (MQ=255)
                                                                                       aGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                <  1:1753632/96‑1 (MQ=255)
                                                                                         cGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                <  1:660260/94‑1 (MQ=255)
                                                                                         cGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                >  2:660260/1‑94 (MQ=255)
                                                                                               tATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGAt                             >  1:1441242/1‑139 (MQ=255)
                                                                                                 tgcatgcGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCg                                            >  1:1956836/1‑122 (MQ=255)
                                                                                                 tgcatgcGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCg                                            <  2:1956836/122‑1 (MQ=255)
                                                                                                      gcgcATTTGCTGGTGGTGAGTAAGTAAAAATcggcg                                                                                                                             >  1:339967/1‑36 (MQ=255)
                                                                                                      gcgcATTTGCTGGTGGTGAGTAAGTAAAAATcggcg                                                                                                                             <  2:339967/36‑1 (MQ=255)
                                                                                                      gcgcATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTAt                                                                                   >  2:203161/1‑78 (MQ=255)
                                                                                                      gcgcATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATcag                                                                                <  1:1233795/81‑3 (MQ=255)
                                                                                                      gcgcATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                >  2:1233795/1‑81 (MQ=255)
                                                                                                      gcgcATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAg                                    >  1:716822/1‑125 (MQ=255)
                                                                                                      gcgcATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAg                                    <  2:716822/125‑1 (MQ=255)
                                                                                                      gcgcATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGc                      >  1:705984/1‑139 (MQ=255)
                                                                                                      gcgcATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGc                      >  1:1532147/1‑139 (MQ=255)
                                                                                                      gcgcATTTGCTGGTGATGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTAt                                                                                   <  1:203161/78‑1 (MQ=255)
                                                                                                       cgcATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCg                     >  2:933488/1‑139 (MQ=255)
                                                                                                         cATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATc                                                                                  <  2:1716160/76‑1 (MQ=255)
                                                                                                         cATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATc                                                                                  >  1:1716160/1‑76 (MQ=255)
                                                                                                         cATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGc                                                                <  2:1597856/94‑1 (MQ=255)
                                                                                                         cATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGc                                                                >  1:1597856/1‑94 (MQ=255)
                                                                                                         cATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTc                   >  2:1975499/1‑139 (MQ=255)
                                                                                                           tttGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                >  1:1610122/1‑76 (MQ=255)
                                                                                                           tttGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                <  2:1610122/76‑1 (MQ=255)
                                                                                                           tttGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAg                 >  1:1740719/1‑139 (MQ=255)
                                                                                                                    ggtgAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgcatcgcatc        >  1:1609306/1‑139 (MQ=255)
                                                                                                                     gtgAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                >  1:1235420/1‑66 (MQ=255)
                                                                                                                     gtgAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                <  2:1235420/66‑1 (MQ=255)
                                                                                                                        agtaagtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATgc                                                                                                   <  1:1382531/44‑1 (MQ=255)
                                                                                                                        agtaagtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATgc                                                                                                   >  2:1382531/1‑44 (MQ=255)
                                                                                                                        agtaagtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                <  1:1723705/63‑1 (MQ=255)
                                                                                                                        agtaagtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                >  2:353940/1‑63 (MQ=255)
                                                                                                                        agtaagtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                <  1:353940/63‑1 (MQ=255)
                                                                                                                        agtaagtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                >  2:1723705/1‑63 (MQ=255)
                                                                                                                        agtaagtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCa                                               <  2:1514331/96‑1 (MQ=255)
                                                                                                                        agtaagtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCa                                               >  1:1514331/1‑96 (MQ=255)
                                                                                                                        agtaagtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGc    >  1:1613326/1‑139 (MQ=255)
                                                                                                                        agtaagtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGc    >  1:922647/1‑139 (MQ=255)
                                                                                                                        agtaagtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGc    <  2:705984/139‑1 (MQ=255)
                                                                                                                        agtaagtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGc    <  2:1609306/139‑1 (MQ=255)
                                                                                                                        agtaagtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGc    <  2:922647/139‑1 (MQ=255)
                                                                                                                        agtaagtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGc    >  1:1070247/1‑139 (MQ=255)
                                                                                                                          taagtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCaa  <  1:933488/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                    
TTGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAA  >  NC_000913/3984065‑3984325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: