Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,992,054	C→T	P301L (CCA→CTA)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,992,054	0	C	T	100.0%	200.0 / NA	61	P301L (CCA→CTA)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/29);  total (32/29)

CCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTA  >  NC_000913/3991924‑3992184                                                                                                                                   |                                                                                                                                   ccATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                            <  2:1938764/139‑1 (MQ=255) ccATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                            <  2:1723547/139‑1 (MQ=255) ccATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                            <  1:1238528/139‑1 (MQ=255)  cATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCat                                                                                                                           >  2:1058680/1‑139 (MQ=255)              tACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCg                                                                                                               <  2:1943016/139‑1 (MQ=255)              tACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCg                                                                                                               >  1:1470899/1‑139 (MQ=255)                     aaCACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATCCTGCATGATGCTGGAGCGTGTTACt                                                                                                        >  1:1041871/1‑139 (MQ=255)                        acTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGaa                                                                                                     >  2:885041/1‑139 (MQ=255)                        acTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGaa                                                                                                     >  1:1651065/1‑139 (MQ=255)                          tgctgctgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGa                                                                                                                  <  2:57534/124‑1 (MQ=255)                          tgctgctgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGa                                                                                                                  >  1:57534/1‑124 (MQ=255)                           gctgctgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCg                                                                                                               <  1:998559/126‑1 (MQ=255)                           gctgctgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCg                                                                                                               >  2:998559/1‑126 (MQ=255)                             tgctgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGATGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCt                                                                                                >  1:1014414/1‑139 (MQ=255)                                tgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGAc                                                                                             <  1:1058680/139‑1 (MQ=255)                                  gAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCTAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACgg                                                                                           <  2:1041871/139‑1 (MQ=255)                                                    tACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                            <  1:1413710/88‑1 (MQ=255)                                                    tACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                            >  2:1413710/1‑88 (MQ=255)                                                     aCCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGaa                                                                                                     <  1:1520899/110‑1 (MQ=255)                                                     aCCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGaa                                                                                                     >  2:1520899/1‑110 (MQ=255)                                                      cccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGt                                                                       <  1:1868659/139‑1 (MQ=255)                                                      cccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGt                                                                       >  2:1868659/1‑139 (MQ=255)                                                       ccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTc                                                                      <  2:1470899/139‑1 (MQ=255)                                                           aCCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCt                                                                     <  1:1161412/136‑1 (MQ=255)                                                           aCCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCt                                                                     >  2:1161412/1‑136 (MQ=255)                                                               aCGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGa                                                                                                                  >  2:973494/1‑87 (MQ=255)                                                               aCGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGa                                                                                                                  <  1:973494/87‑1 (MQ=255)                                                                      ctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTg                                                                                                                    >  2:1073895/1‑78 (MQ=255)                                                                      ctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTg                                                                                                                    <  1:1073895/78‑1 (MQ=255)                                                                      ctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTgg                                                                   >  1:588717/1‑127 (MQ=255)                                                                      ctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTgg                                                                   <  2:588717/127‑1 (MQ=255)                                                                           gAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCgctgc                                                  >  1:872038/1‑139 (MQ=255)                                                                             aaGATATCAAACAGCGTTTGCACGATGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                                <  2:1014414/139‑1 (MQ=255)                                                                             aaGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                                <  1:885041/139‑1 (MQ=255)                                                                             aaGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                                <  2:1651065/139‑1 (MQ=255)                                                                             aaGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                                <  1:1039453/139‑1 (MQ=255)                                                                              aGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                                >  1:1346983/1‑138 (MQ=255)                                                                              aGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                                <  2:1346983/138‑1 (MQ=255)                                                                                      aaCAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCgtg                                                                                                             <  1:1645209/69‑1 (MQ=255)                                                                                      aaCAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCgtg                                                                                                             >  2:1645209/1‑69 (MQ=255)                                                                                          gCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCt                                                                     <  2:1248096/105‑1 (MQ=255)                                                                                          gCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCt                                                                     >  1:1248096/1‑105 (MQ=255)                                                                                           cGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAgtgt                                  >  1:1138871/1‑139 (MQ=255)                                                                                           cGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATACTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAgtgt                                  >  2:1776364/1‑139 (MQ=255)                                                                                             tttGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTa                                                                            >  1:1717272/1‑95 (MQ=255)                                                                                             tttGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTa                                                                            <  2:1717272/95‑1 (MQ=255)                                                                                             tttGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                                <  1:155726/123‑1 (MQ=255)                                                                                             tttGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                                >  2:155726/1‑123 (MQ=255)                                                                                               tGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCgtgt                                                                                                            <  2:2038063/61‑1 (MQ=255)                                                                                               tGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCgtgt                                                                                                            >  1:2038063/1‑61 (MQ=255)                                                                                                      cgGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                            <  1:151259/38‑1 (MQ=255)                                                                                                      cgGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                            >  2:151259/1‑38 (MQ=255)                                                                                                         cGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGc                    >  2:597108/1‑139 (MQ=255)                                                                                                              ttGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTaaa                                       >  1:558069/1‑115 (MQ=255)                                                                                                              ttGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTaaa                                       <  2:558069/115‑1 (MQ=255)                                                                                                                gTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCg                                                                                                               <  1:1187786/41‑1 (MQ=255)                                                                                                                gTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCg                                                                                                               >  2:1187786/1‑41 (MQ=255)                                                                                                                        tGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTGTTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGc     >  1:1294884/1‑139 (MQ=255)                                                                                                                        tGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGc     >  1:862471/1‑139 (MQ=255)                                                                                                                        tGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGc     >  2:1192330/1‑139 (MQ=255)                                                                                                                           tctcGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTa  >  1:1298641/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                   CCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTA  >  NC_000913/3991924‑3992184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑