Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	37,030	A→G	V265V (GTT→GTC)	caiC ←	putative crotonobetaine/carnitine‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	37,030	0	A	G	100.0%	230.0 / NA	63	V265V (GTT→GTC)	caiC	putative crotonobetaine/carnitine‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/32);  total (31/32)

TCACAAAACGCATCTTTTTCCTGCTCCGACAAGTTGAGATAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTAACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCA  >  NC_000913/36899‑37163                                                                                                                                    |                                                                                                                                      tCACAAAACGCATCTTTTTCCTGCTCCGACAAGTTGAGATAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTgg                                                                                                                                <  1:520717/139‑1 (MQ=255)   acaAAACGCATCTTTTTCCTGCTCCGACAAGTTGAGATAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGGTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGgcg                                                                                                                              <  1:680819/139‑1 (MQ=255)        aCGCATCTTTTTCCTGCTCCGACAAGTTGAGATAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTa                                                                                                                         >  2:971287/1‑139 (MQ=255)                 tttCCTGCTCCGACAAGTTGAGATAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTAc                                                                                                                >  1:569705/1‑139 (MQ=255)                        cTCCGACAAGTTGAGATAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTccc                                                                                                         >  2:1773458/1‑139 (MQ=255)                                  ttGAGATAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTc                                                                                                           <  2:1456806/127‑1 (MQ=255)                                  ttGAGATAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTc                                                                                                           >  1:1456806/1‑127 (MQ=255)                                     agaTAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCggg                                                                                            <  2:569705/139‑1 (MQ=255)                                       aTAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGt                                                                                                                          <  1:1075432/107‑1 (MQ=255)                                       aTAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGt                                                                                                                          >  2:1075432/1‑107 (MQ=255)                                        tAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGgcgc                                                                                         <  1:1279967/139‑1 (MQ=255)                                                cACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTtc                                                                                 <  1:2000141/139‑1 (MQ=255)                                                     cccGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGAc                                                                            >  2:1445778/1‑139 (MQ=255)                                                               gtgtTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAg                                                                  >  1:796366/1‑139 (MQ=255)                                                                tgtTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGgcg                                                                                                                              >  1:697231/1‑78 (MQ=255)                                                                tgtTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGgcg                                                                                                                              <  2:697231/78‑1 (MQ=255)                                                                      tGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCt                                                                                                              <  1:657934/88‑1 (MQ=255)                                                                      tGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCt                                                                                                              >  2:657934/1‑88 (MQ=255)                                                                        aTCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGAc                                                                            <  2:1730734/120‑1 (MQ=255)                                                                        aTCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGAc                                                                            >  1:1730734/1‑120 (MQ=255)                                                                          cGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACGTTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGc                                                       >  1:1265221/1‑139 (MQ=255)                                                                          cGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGc                                                       >  2:1700319/1‑139 (MQ=255)                                                                            ttCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCgg                                                                                                                           <  1:783872/69‑1 (MQ=255)                                                                            ttCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCgg                                                                                                                           >  2:783872/1‑69 (MQ=255)                                                                            ttCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTtc                                                                                 >  2:622384/1‑111 (MQ=255)                                                                            ttCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTtc                                                                                 <  1:622384/111‑1 (MQ=255)                                                                            ttCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAg                                                     >  1:1927392/1‑139 (MQ=255)                                                                              cGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGag                                                   <  2:1265221/139‑2 (MQ=255)                                                                                  gAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAAcg                                               <  2:1927392/139‑1 (MQ=255)                                                                                    aGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAAcgcc                                             >  1:1986073/1‑139 (MQ=255)                                                                                         gcTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCggg                                                                                            >  2:50022/1‑87 (MQ=255)                                                                                         gcTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCggg                                                                                            <  1:50022/87‑1 (MQ=255)                                                                                             tACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGgcg                                                                                          <  1:2021351/85‑1 (MQ=255)                                                                                             tACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGgcg                                                                                          >  2:2021351/1‑85 (MQ=255)                                                                                             tACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATcgcc                                    >  1:647069/1‑139 (MQ=255)                                                                                             tACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATcg                                      <  2:509479/137‑1 (MQ=255)                                                                                             tACCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATcg                                      >  1:509479/1‑137 (MQ=255)                                                                                              aCCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATcgccg                                   <  1:1773458/139‑1 (MQ=255)                                                                                              aCCATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATcgccg                                   <  1:971287/139‑1 (MQ=255)                                                                                                cATCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCa                                 <  2:378602/139‑1 (MQ=255)                                                                                                 aTCAACGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAg                                <  2:1986073/139‑1 (MQ=255)                                                                                                     aCGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCggg                                                                                            >  2:210306/1‑75 (MQ=255)                                                                                                     aCGTACGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCggg                                                                                            <  1:210306/75‑1 (MQ=255)                                                                                                         aCGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCggg                                                                                            >  1:1333095/1‑71 (MQ=255)                                                                                                         aCGGATCATCATCGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCggg                                                                                            <  2:1333095/71‑1 (MQ=255)                                                                                                                atcatcGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGAc                                                                            <  1:104650/80‑1 (MQ=255)                                                                                                                atcatcGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGAc                                                                            <  1:639072/80‑1 (MQ=255)                                                                                                                atcatcGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGAc                                                                            >  2:104650/1‑80 (MQ=255)                                                                                                                atcatcGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGAc                                                                            >  2:639072/1‑80 (MQ=255)                                                                                                                atcatcGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGa                 >  1:2038374/1‑139 (MQ=255)                                                                                                                 tcatcGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGAc                                                                            >  2:853771/1‑79 (MQ=255)                                                                                                                 tcatcGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGAc                                                                            <  1:853771/79‑1 (MQ=255)                                                                                                                    tcGGAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATg             <  2:2038374/139‑1 (MQ=255)                                                                                                                       gAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTg                                                                                       <  2:18805/62‑1 (MQ=255)                                                                                                                       gAATACATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTg                                                                                       >  1:18805/1‑62 (MQ=255)                                                                                                                           aCATTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCa      <  1:1445778/139‑1 (MQ=255)                                                                                                                             aTTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAAcgcc                                             <  2:477014/98‑1 (MQ=255)                                                                                                                             aTTCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAAcgcc                                             >  1:477014/1‑98 (MQ=255)                                                                                                                               tCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAg                                                                  >  2:413835/1‑75 (MQ=255)                                                                                                                               tCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAg                                                                  <  1:413835/75‑1 (MQ=255)                                                                                                                               tCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCAt                                        >  1:409632/1‑101 (MQ=255)                                                                                                                               tCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCAt                                        <  2:409632/101‑1 (MQ=255)                                                                                                                               tCGGTGACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCa  >  1:160690/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                      TCACAAAACGCATCTTTTTCCTGCTCCGACAAGTTGAGATAAAACATCACTTCCCGCAGGCGGTGTTGCTGATCGTTCGCTGAAGGCGGCTGTACCATCAACGTACGGATCATCATCGGAATACATTCGGTAACGGTGGCGCGGTACTTCTGTACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCGCCATCGCCGCAGTACACTGGCAATCGATATGAAACGCAGGCA  >  NC_000913/36899‑37163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑