| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,231,237 | A→G | intergenic (+11/+122) | rluF → / ← yjbD | 23S rRNA pseudouridine(2604) synthase/DUF3811 family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,231,237 | 0 | A | G | 94.9% | 122.8 / ‑2.3 | 39 | intergenic (+11/+122) | rluF/yjbD | 23S rRNA pseudouridine(2604) synthase/DUF3811 family protein |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (16/21); total (17/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-01 | |||||||||||
ACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGAT > NC_000913/4231110‑4231359 | aCAGCGGGCATCAAACGTCCAGTCGTTAAGATGG‑AAAAACGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGaaaa < 1:525603/139‑1 (MQ=255) aGTCGTTAAGATGGAAAAAACGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGa < 2:33436/139‑1 (MQ=255) gTCGTTAAGATGGAAAAAACGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGAc > 1:1487017/1‑139 (MQ=255) tCGTTAAGATGGAAAAAACGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACa > 2:1239083/1‑139 (MQ=255) ggAAAAAACGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGc > 1:1418197/1‑139 (MQ=255) ggAAAAAACGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGc > 2:523709/1‑139 (MQ=255) ggAAAAAACGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGc < 2:1119782/139‑1 (MQ=255) cggAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaa < 1:1128778/139‑1 (MQ=255) cggAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaa < 1:1239083/139‑1 (MQ=255) cggAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaa < 2:1418197/139‑1 (MQ=255) cggAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaa < 1:417623/139‑1 (MQ=255) cggAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaa < 1:523709/139‑1 (MQ=255) cGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAgg > 2:749212/1‑139 (MQ=255) gTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGt < 1:749212/139‑1 (MQ=255) gTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaa < 2:990532/109‑1 (MQ=255) gTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaa > 1:990532/1‑109 (MQ=255) tAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTca > 1:72809/1‑139 (MQ=255) cTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTc < 2:1866900/126‑1 (MQ=255) cTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTc > 1:1866900/1‑126 (MQ=255) cTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTg < 2:1487017/139‑1 (MQ=255) cGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaaaa > 1:1721782/1‑96 (MQ=255) cGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaaaa < 2:1721782/96‑1 (MQ=255) gCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACa < 1:1024733/72‑1 (MQ=255) gCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACa > 2:1024733/1‑72 (MQ=255) gggCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAAtct < 2:1764144/139‑1 (MQ=255) gggCGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAAtct < 2:72809/139‑1 (MQ=255) cGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaa > 2:429625/1‑86 (MQ=255) cGTAAAAAGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaa < 1:429625/86‑1 (MQ=255) aaaaaGAAGGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCg < 2:7044/139‑1 (MQ=255) aagGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaa > 1:404653/1‑77 (MQ=255) aagGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaa < 2:404653/77‑1 (MQ=255) agGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaa > 2:429470/1‑76 (MQ=255) agGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaa < 1:429470/76‑1 (MQ=255) agGGGCGCTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATtg > 2:1586255/1‑139 (MQ=255) cgcTGATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGAt > 1:1391389/1‑139 (MQ=255) cgcTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAAtct < 2:1725196/121‑1 (MQ=255) cgcTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAAtct > 1:1725196/1‑121 (MQ=255) gATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaa < 2:930467/67‑1 (MQ=255) gATTAACGCCTCGGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGaa > 1:930467/1‑67 (MQ=255) | ACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGGAAAAAGGCGGTCGCCCGGCGTCCAACGGTAAGCGTTTTACCTCGCCGGGGCGTAAAAAGAAGGGGCGCTGATTAACGCCTCAGACTGATGAAAACGGCCCAAAAGTGCCCGGACAGTCCCCTCGCCCCTTCGGGAAAAGGGTTAGGGTGAGGGGAAAATTGTGTTCACACTGACGAGTGAAAATCTCAGGCGTTGATTGTGGAT > NC_000913/4231110‑4231359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |