Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,351,706 A→G intergenic (‑44/‑137) dcuS ← / → yjdI sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration/putative 4Fe‑4S mono‑cluster protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,351,7060AG100.0% 326.9 / NA 93intergenic (‑44/‑137)dcuS/yjdIsensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration/putative 4Fe‑4S mono‑cluster protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/49);  total (44/49)

GAGAACAGTACCGCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGAAGGT  >  NC_000913/4351571‑4351841
                                                                                                                                       |                                                                                                                                       
gagaACAGTACCGCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGc                                                                                                                                      >  2:1552721/1‑139 (MQ=255)
    aCAGTACCGCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGt                                                                                                                                  <  2:329818/139‑1 (MQ=255)
         aCCGCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTc                                                                                                                             >  2:471766/1‑139 (MQ=255)
            gCACTGACCATTATGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCGCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCaaa                                                                                                                          <  2:1414204/139‑1 (MQ=255)
            gCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCaaa                                                                                                                          <  2:141774/139‑1 (MQ=255)
            gCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCaaa                                                                                                                          <  2:1603064/139‑1 (MQ=255)
            gCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCaaa                                                                                                                          <  2:1792375/139‑1 (MQ=255)
             cACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCggg                                                                                                                                   >  1:1941393/1‑129 (MQ=255)
             cACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCggg                                                                                                                                   <  2:1941393/129‑1 (MQ=255)
             cACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCaaa                                                                                                                          <  1:1800580/138‑1 (MQ=255)
             cACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCaaa                                                                                                                          >  2:1800580/1‑138 (MQ=255)
             cACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAg                                                                                                                         >  2:933564/1‑139 (MQ=255)
               cTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAggg                                                                                                                       <  1:1552721/139‑1 (MQ=255)
                  aCCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGt                                                                                                                    >  1:1628080/1‑139 (MQ=255)
                  aCCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGt                                                                                                                    >  2:1557703/1‑139 (MQ=255)
                  aCCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGt                                                                                                                    <  1:1995880/139‑1 (MQ=255)
                  aCCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGt                                                                                                                    >  2:1995880/1‑139 (MQ=255)
                  aCCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGt                                                                                                                    >  2:37530/1‑139 (MQ=255)
                  aCCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGt                                                                                                                    >  1:1587265/1‑139 (MQ=255)
                           aTCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGAt                                                                                                           <  1:933564/139‑1 (MQ=255)
                           aTCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGAt                                                                                                           <  2:1691517/139‑1 (MQ=255)
                           aTCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGAt                                                                                                           <  2:1587265/139‑1 (MQ=255)
                            tCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGAtt                                                                                                          >  2:49318/1‑139 (MQ=255)
                              aCTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTgg                                                                                                        <  1:37530/139‑1 (MQ=255)
                                 gtgGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGt                                                                                                                                  >  2:1155569/1‑110 (MQ=255)
                                 gtgGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGt                                                                                                                                  <  1:1155569/110‑1 (MQ=255)
                                     tACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTgg                                                                                                        >  2:393405/1‑132 (MQ=255)
                                     tACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTgg                                                                                                        <  1:393405/132‑1 (MQ=255)
                                          aaTTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTgg                                                                                                        <  2:1393190/127‑1 (MQ=255)
                                          aaTTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTgg                                                                                                        >  1:1393190/1‑127 (MQ=255)
                                          aaTTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGa                                                                                            >  1:1714482/1‑139 (MQ=255)
                                            tttCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCtata                                                                                                   <  2:1506493/130‑1 (MQ=255)
                                            tttCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCtata                                                                                                   >  1:1506493/1‑130 (MQ=255)
                                            tttCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAag                                                                                          >  2:917407/1‑139 (MQ=255)
                                            tttCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAag                                                                                          >  2:864512/1‑139 (MQ=255)
                                            tttCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAag                                                                                          >  1:804982/1‑139 (MQ=255)
                                            tttCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAag                                                                                          <  1:49318/139‑1 (MQ=255)
                                                 tCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGAct                                                                                     >  1:1035698/1‑139 (MQ=255)
                                                    gACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTgg                                                                                                        >  2:1946251/1‑117 (MQ=255)
                                                    gACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTgg                                                                                                        <  1:1946251/117‑1 (MQ=255)
                                                      cGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCggg                                                                                                                                   <  2:1338543/88‑1 (MQ=255)
                                                      cGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCggg                                                                                                                                   >  1:1338543/1‑88 (MQ=255)
                                                             gTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGaa                                                                         <  2:1628080/139‑1 (MQ=255)
                                                              tAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCaaa                                                                                                                          <  2:2029574/89‑1 (MQ=255)
                                                              tAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCaaa                                                                                                                          >  1:2029574/1‑89 (MQ=255)
                                                              tAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGaa                                                                                           <  1:1933639/120‑1 (MQ=255)
                                                              tAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGaa                                                                                           >  2:1933639/1‑120 (MQ=255)
                                                                   tGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGcc                                                                   >  1:1876838/1‑139 (MQ=255)
                                                                   tGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGc                                                                    >  1:157331/1‑138 (MQ=255)
                                                                   tGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGc                                                                    <  2:157331/138‑1 (MQ=255)
                                                                            gCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCaaa                                                                                                                          <  2:369834/75‑1 (MQ=255)
                                                                            gCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCaaa                                                                                                                          >  1:369834/1‑75 (MQ=255)
                                                                             cAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCa                                                                  <  2:326953/130‑1 (MQ=255)
                                                                             cAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCa                                                                  >  1:326953/1‑130 (MQ=255)
                                                                                tgaatgTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAgg                                                                                                                        >  2:275556/1‑73 (MQ=255)
                                                                                tgaatgTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAgg                                                                                                                        <  1:275556/73‑1 (MQ=255)
                                                                                tgaatgTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCa                                                                  >  1:756977/1‑127 (MQ=255)
                                                                                tgaatgTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCa                                                                  <  2:756977/127‑1 (MQ=255)
                                                                                     gTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTc                                                 <  1:864512/139‑1 (MQ=255)
                                                                                      tctcATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAggg                                                                                                                       <  1:236467/68‑1 (MQ=255)
                                                                                      tctcATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAggg                                                                                                                       >  2:236467/1‑68 (MQ=255)
                                                                                           tcaGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGc                                           <  1:1557703/139‑1 (MQ=255)
                                                                                               cTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATg                                       >  2:179042/1‑139 (MQ=255)
                                                                                                    ttGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCa                                                                  <  2:631297/107‑1 (MQ=255)
                                                                                                    ttGTGTGACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCa                                                                  >  1:631297/1‑107 (MQ=255)
                                                                                                    ttGTGTGACAAATTTCTTAAGCATTAGCTCTGATGGGGCGGCTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGCAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTtat                                  <  1:1556985/139‑1 (MQ=255)
                                                                                                       tgtgACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGc                               <  2:1035698/139‑1 (MQ=255)
                                                                                                       tgtgACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGc                               <  1:917407/139‑1 (MQ=255)
                                                                                                       tgtgACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGc                               <  1:471766/139‑1 (MQ=255)
                                                                                                         tgACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGa                                                                          <  2:595002/94‑1 (MQ=255)
                                                                                                         tgACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGa                                                                          >  1:595002/1‑94 (MQ=255)
                                                                                                         tgACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGc                                                              <  2:363222/106‑1 (MQ=255)
                                                                                                         tgACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGc                                                              >  1:363222/1‑106 (MQ=255)
                                                                                                         tgACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGc                                           <  1:1002035/125‑1 (MQ=255)
                                                                                                         tgACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGc                                           >  2:1002035/1‑125 (MQ=255)
                                                                                                         tgACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGa                             >  1:1673448/1‑139 (MQ=255)
                                                                                                          gACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCtata                                                                                                   >  1:797688/1‑68 (MQ=255)
                                                                                                          gACAAATTTCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCtata                                                                                                   <  2:797688/68‑1 (MQ=255)
                                                                                                                 ttCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTg                                                               <  2:416861/97‑1 (MQ=255)
                                                                                                                 ttCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTg                                                               >  1:416861/1‑97 (MQ=255)
                                                                                                                 ttCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGc                                           >  1:1449464/1‑117 (MQ=255)
                                                                                                                 ttCTTAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGc                                           <  2:1449464/117‑1 (MQ=255)
                                                                                                                     tAAGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACtga                 <  2:1908891/139‑1 (MQ=255)
                                                                                                                      aaGCATTATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACtgat                <  2:804982/139‑1 (MQ=255)
                                                                                                                           ttATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACTGATgagag           >  1:382731/1‑139 (MQ=255)
                                                                                                                            tATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGaa                                                                         <  2:195338/76‑1 (MQ=255)
                                                                                                                            tATCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGaa                                                                         >  1:195338/1‑76 (MQ=255)
                                                                                                                             aTCTCTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGagagag         >  1:1774837/1‑139 (MQ=255)
                                                                                                                              tctcTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATA‑‑‑GAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATa                     <  2:1122293/123‑1 (MQ=255)
                                                                                                                              tctcTGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATA‑‑‑GAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATa                     >  1:1122293/1‑123 (MQ=255)
                                                                                                                               ctctGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAggg       <  2:1714482/139‑1 (MQ=255)
                                                                                                                               ctctGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAggg       <  1:179042/139‑1 (MQ=255)
                                                                                                                                tctGATGGGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGa      <  2:382731/139‑1 (MQ=255)
                                                                                                                                  tgatggGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTTTGAAGATTCCGGGGc                                           >  2:447930/1‑100 (MQ=255)
                                                                                                                                  tgatggGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTTTGAAGATTCCGGGGc                                           <  1:447930/100‑1 (MQ=255)
                                                                                                                                   gatggGGCGGGTGATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGAAgg   >  1:1706616/1‑139 (MQ=255)
                                                                                                                                    atggGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGAAGGt  >  2:1853052/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                       
GAGAACAGTACCGCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATTGGCTATATAGGGGAAGAGACTCTGGCAACGGAAACTGCCAGTGCTGTATGAAGATTCCGGGGCTATGCTTATAGCGATAATCATACTGATGAGAGAGGGAAGGT  >  NC_000913/4351571‑4351841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: