Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,386,872	C→T	A833A (GCG→GCA)	mscM ←	mechanosensitive channel protein, miniconductance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,386,872	0	C	T	100.0%	156.6 / NA	48	A833A (GCG→GCA)	mscM	mechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/25);  total (23/25)

TGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCA  >  NC_000913/4386739‑4386999                                                                                                                                      |                                                                                                                                tggtgatggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgcactgcacc                                                                                                                            >  2:1058479/1‑139 (MQ=255)      atggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAggg                                                                                                                       >  1:354312/1‑139 (MQ=255)                 ggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTcc                                                                                                            <  1:2053640/139‑1 (MQ=255)                  gCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCa                                                                                                           <  1:707521/139‑1 (MQ=255)                          cGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                   <  1:564214/139‑1 (MQ=255)                            tACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTAc                                                                                                 >  2:1359465/1‑139 (MQ=255)                              cccGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGc                                                                                               <  1:1058479/139‑1 (MQ=255)                                 ggCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTc                                                                                                             <  1:62128/122‑1 (MQ=255)                                 ggCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTc                                                                                                             >  2:62128/1‑122 (MQ=255)                                   cGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                   >  1:1652716/1‑130 (MQ=255)                                   cGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                   <  2:1652716/130‑1 (MQ=255)                                   cGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGt                                                                                          >  2:362214/1‑139 (MQ=255)                                    gTTAAATCCAGGTGCTGTAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCAATGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGtc                                                                                         <  2:1264442/139‑2 (MQ=255)                                      tagaTCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCCGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTAcc                                                                                       <  1:1359465/136‑1 (MQ=255)                                      tAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTAcc                                                                                       >  1:1037017/1‑139 (MQ=255)                                      tAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTAcc                                                                                       <  2:1037017/139‑1 (MQ=255)                                         aTCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc                                                                                              <  1:572279/129‑1 (MQ=255)                                         aTCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc                                                                                              >  2:572279/1‑129 (MQ=255)                                             aGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAgg                                                                                <  2:1801509/139‑1 (MQ=255)                                               gTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTg                                                                              <  1:362214/139‑1 (MQ=255)                                                   tgcAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACAt                                                                          >  2:1309704/1‑139 (MQ=255)                                                    gcAAAATCGCCGGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATc                                                                         >  1:1193384/1‑139 (MQ=255)                                                    gcAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATc                                                                         >  2:137300/1‑139 (MQ=255)                                                            gCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCg                                                                 <  1:1309704/139‑1 (MQ=255)                                                                            cgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGGATGGCAATCAgcactgcacc                                                                                                                            <  1:325545/64‑1 (MQ=255)                                                                            cgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGGATGGCAATCAgcactgcacc                                                                                                                            >  2:325545/1‑64 (MQ=255)                                                                                         cACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCca                                                                      <  2:1765457/105‑1 (MQ=255)                                                                                         cACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCca                                                                      >  1:1765457/1‑105 (MQ=255)                                                                                           ccAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATg                                  <  1:1666484/139‑1 (MQ=255)                                                                                              gCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGAt                               >  1:405336/1‑139 (MQ=255)                                                                                                 gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTc                            >  2:635054/1‑139 (MQ=255)                                                                                                    tggtgATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc                                                                                              <  1:404715/70‑1 (MQ=255)                                                                                                    tggtgATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc                                                                                              >  2:404715/1‑70 (MQ=255)                                                                                                    tggtgATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGa                         >  2:1529880/1‑139 (MQ=255)                                                                                                      gtgATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTgg                                                                                   >  2:1204283/1‑79 (MQ=255)                                                                                                      gtgATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTgg                                                                                   <  1:1204283/79‑1 (MQ=255)                                                                                                      gtgATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGAcc                       <  2:405336/139‑1 (MQ=255)                                                                                                      gggatgatAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGAcc                       <  2:1193384/137‑1 (MQ=255)                                                                                                         atgatAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACcaca                    >  1:9104/1‑139 (MQ=255)                                                                                                         atgatAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACcac                     <  2:904756/138‑1 (MQ=255)                                                                                                         atgatAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACcac                     >  1:904756/1‑138 (MQ=255)                                                                                                            atAAACACCAGAATGGCAATCAGCACTGCACTGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACcac                     >  2:142114/1‑135 (MQ=255)                                                                                                            atAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTg                                                                                    >  2:406691/1‑72 (MQ=255)                                                                                                            atAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTg                                                                                    <  1:406691/72‑1 (MQ=255)                                                                                                            atAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACcac                     <  1:142114/135‑1 (MQ=255)                                                                                                                   ccAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAg                   >  2:1579905/1‑130 (MQ=255)                                                                                                                   ccAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAg                   <  1:1579905/130‑1 (MQ=255)                                                                                                                      gAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGa       >  1:930045/1‑139 (MQ=255)                                                                                                                         tGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAg                                         <  2:424465/102‑1 (MQ=255)                                                                                                                         tGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAg                                         >  1:424465/1‑102 (MQ=255)                                                                                                                           gCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCa  <  2:354312/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                TGGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCA  >  NC_000913/4386739‑4386999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑