Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,395,058	C→T	G331G (GGC→GGT)	nnr →	bifunctional NAD(P)H‑hydrate repair enzyme, C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,395,058	0	C	T	100.0%	147.6 / NA	46	G331G (GGC→GGT)	nnr	bifunctional NAD(P)H‑hydrate repair enzyme, C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/20);  total (26/20)

GTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCA  >  NC_000913/4394924‑4395189                                                                                                                                       |                                                                                                                                    gTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGc                                                                                                                                 <  2:402021/139‑1 (MQ=255)      gACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAagag                                                                                                                            >  1:344374/1‑139 (MQ=255)          cGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGCCGATGGACTCTCTTACCCAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTggg                                                                                                                        >  1:1856310/1‑139 (MQ=255)          cGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTggg                                                                                                                        >  2:1890431/1‑139 (MQ=255)           gCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTgggg                                                                                                                       <  2:838777/139‑1 (MQ=255)                         gcgcCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGc                                                                                                         >  2:1860804/1‑139 (MQ=255)                                         cACGACCGGAATTAATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAAttt                                                                                         >  1:1381291/1‑139 (MQ=255)                                            gACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCg                                                                                      <  2:344374/139‑1 (MQ=255)                                            gACCGGAATTGACGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCg                                                                                      <  1:783362/139‑1 (MQ=255)                                                          gTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtgttgt                                                                        >  2:1520367/1‑139 (MQ=255)                                                            gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTgg                                                                      >  2:1139025/1‑139 (MQ=255)                                                                gAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTAGCAAACCGATGTTGTGGGATg                                                                  >  1:1200520/1‑139 (MQ=255)                                                                   cTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCg                                                               <  1:677284/139‑1 (MQ=255)                                                                   cTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCg                                                               <  1:1139025/139‑1 (MQ=255)                                                                     gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                                                    <  2:497813/132‑1 (MQ=255)                                                                     gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                                                    >  1:497813/1‑132 (MQ=255)                                                                     gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGAt                                                             >  2:1168812/1‑139 (MQ=255)                                                                      aCGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCg                                                                               >  2:1168552/1‑120 (MQ=255)                                                                      aCGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCg                                                                               <  1:1168552/120‑1 (MQ=255)                                                                      aCGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                                                    <  1:1915320/131‑1 (MQ=255)                                                                      aCGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                                                    >  2:1915320/1‑131 (MQ=255)                                                                      aCGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATg                                                            >  2:709454/1‑139 (MQ=255)                                                                                ctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACct                                                  >  2:407634/1‑139 (MQ=255)                                                                                      ccGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGc                                            >  1:1724753/1‑139 (MQ=255)                                                                                         aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTATGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                         >  1:252305/1‑139 (MQ=255)                                                                                           aGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTgg                                                                                                                         >  2:1104284/1‑57 (MQ=255)                                                                                           aGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTgg                                                                                                                         <  1:1104284/57‑1 (MQ=255)                                                                                           aGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatca                                       <  1:709454/139‑1 (MQ=255)                                                                                             ccTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatcaat                                     <  2:378681/139‑1 (MQ=255)                                                                                                   aTGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGAt                               <  1:1860804/139‑1 (MQ=255)                                                                                                            tgtgGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTcac                      >  1:1810709/1‑139 (MQ=255)                                                                                                             gtggtggtgATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTcaca                     <  2:1200520/139‑1 (MQ=255)                                                                                                                    tgATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGt              <  1:1168812/139‑1 (MQ=255)                                                                                                                      aTTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAcc                                                                                >  1:1585142/1‑71 (MQ=255)                                                                                                                      aTTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAcc                                                                                <  2:1585142/71‑1 (MQ=255)                                                                                                                        tGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATc          >  2:197098/1‑139 (MQ=255)                                                                                                                          gTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCg                                                                               <  1:1507663/68‑1 (MQ=255)                                                                                                                          gTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCg                                                                               >  2:1507663/1‑68 (MQ=255)                                                                                                                           tCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtgttg                                                                         >  1:373810/1‑73 (MQ=255)                                                                                                                           tCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtgttg                                                                         <  2:373810/73‑1 (MQ=255)                                                                                                                             ccGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCg                                                               <  2:152380/81‑1 (MQ=255)                                                                                                                             ccGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCg                                                               >  1:152380/1‑81 (MQ=255)                                                                                                                             ccGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcgcc     >  2:1783798/1‑139 (MQ=255)                                                                                                                                gTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTgg                                             >  1:851917/1‑96 (MQ=255)                                                                                                                                gTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTgg                                             <  2:851917/96‑1 (MQ=255)                                                                                                                                gTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCACTCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCa  >  1:1188610/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                    GTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCCGCA  >  NC_000913/4394924‑4395189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑