Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,443,244 A→G K288R (AAG→AGG)  tamA → translocation and assembly module for autotransporter export, outer membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,443,2440AG100.0% 306.7 / NA 87K288R (AAG→AGG) tamAtranslocation and assembly module for autotransporter export, outer membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/50);  total (37/50)

AATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTGAAGAATCCACTGGAACAATATTATTTGGTGCAGG  >  NC_000913/4443112‑4443375
                                                                                                                                    |                                                                                                                                   
aaTTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTg                                                                                                                               >  1:2056185/1‑139 (MQ=255)
 aTTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTgg                                                                                                                              <  2:1904364/139‑1 (MQ=255)
      aTAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGaa                                                                                                                         <  1:793913/139‑1 (MQ=255)
       tAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAAc                                                                                                                        <  2:1256639/139‑1 (MQ=255)
       tAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAAc                                                                                                                        >  1:480517/1‑139 (MQ=255)
        aaaGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAAct                                                                                                                       <  2:480517/139‑1 (MQ=255)
        aaaGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAAct                                                                                                                       <  1:827193/139‑1 (MQ=255)
            cgcgcgAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTa                                                                                                                   >  1:1137543/1‑139 (MQ=255)
            cgcgcgAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTa                                                                                                                   >  2:1156448/1‑139 (MQ=255)
              cgcgAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTgg                                                                                                                              <  2:1513484/126‑1 (MQ=255)
              cgcgAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTgg                                                                                                                              >  1:1513484/1‑126 (MQ=255)
                cgaaacgaaaGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGt                                                                                                               <  2:1716647/139‑1 (MQ=255)
                cgaaacgaaaGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGt                                                                                                               <  1:1773553/139‑1 (MQ=255)
                         aGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTg                                                                                                      <  2:492766/139‑1 (MQ=255)
                             ttACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTa                                                                                                                   <  2:1647288/122‑1 (MQ=255)
                             ttACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTa                                                                                                                   >  1:1647288/1‑122 (MQ=255)
                              tACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGaccac                                                                                                 <  2:2056185/139‑1 (MQ=255)
                              tACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGaccac                                                                                                 <  1:860532/139‑1 (MQ=255)
                                   ttGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTg                                                                                                      <  2:351963/129‑1 (MQ=255)
                                   ttGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTg                                                                                                      >  1:351963/1‑129 (MQ=255)
                                    tGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGt                                                                                                               <  2:1629687/119‑1 (MQ=255)
                                    tGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGt                                                                                                               >  1:1629687/1‑119 (MQ=255)
                                     gACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTa                                                                                                                   <  1:171797/114‑1 (MQ=255)
                                     gACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTa                                                                                                                   >  2:171797/1‑114 (MQ=255)
                                          gCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGt                                                                                             >  2:1935387/1‑131 (MQ=255)
                                          gCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGt                                                                                             <  1:1935387/131‑1 (MQ=255)
                                           cGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTAtt                                                                                    >  2:677685/1‑139 (MQ=255)
                                               gTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCcg                                                                                <  2:561139/139‑1 (MQ=255)
                                                        cgAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTcaca                                                                                                           <  2:535208/103‑1 (MQ=255)
                                                        cgAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTcaca                                                                                                           >  1:535208/1‑103 (MQ=255)
                                                        cgAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAAc                                                                       <  2:975205/139‑1 (MQ=255)
                                                         gAACAGAAACCACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTa                                                                                                                   <  1:922479/94‑1 (MQ=255)
                                                         gAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAATGCGACGTGGAAAAGGCCGTGGATGAACTCTTa                                                                                                                   >  2:922479/1‑94 (MQ=255)
                                                          aaCAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAg                                                                     >  1:1409695/1‑139 (MQ=255)
                                                                   cacCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGAc                                                            <  2:385292/139‑1 (MQ=255)
                                                                     ccATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACtt                                                          <  2:87218/139‑1 (MQ=255)
                                                                     ccATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACtt                                                          <  1:1156448/139‑1 (MQ=255)
                                                                       aTCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGa                                                                    <  2:599973/127‑1 (MQ=255)
                                                                       aTCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGa                                                                    >  1:599973/1‑127 (MQ=255)
                                                                        tCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATCGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAg                                                       <  2:1758393/139‑1 (MQ=255)
                                                                         cGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGc                                                      >  2:463529/1‑139 (MQ=255)
                                                                         cGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGc                                                      >  1:282987/1‑139 (MQ=255)
                                                                         cGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGc                                                      >  2:671906/1‑139 (MQ=255)
                                                                         cGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACCCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGc                                                      >  1:1955389/1‑139 (MQ=255)
                                                                          gAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGt                                                                                                               >  1:842784/1‑81 (MQ=255)
                                                                          gAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGt                                                                                                               <  2:842784/81‑1 (MQ=255)
                                                                           aaaCCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAAct                                                                                                                       <  1:524605/72‑1 (MQ=255)
                                                                           aaaCCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAAct                                                                                                                       >  2:524605/1‑72 (MQ=255)
                                                                           aaaCCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCta                                                    >  1:608149/1‑139 (MQ=255)
                                                                                  ggTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTa                                                                                                                   >  2:1560867/1‑69 (MQ=255)
                                                                                  ggTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTa                                                                                                                   <  1:1560867/69‑1 (MQ=255)
                                                                                  ggTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTAACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATg                                             >  2:1076337/1‑139 (MQ=255)
                                                                                     cGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCg                                          <  2:1955389/139‑1 (MQ=255)
                                                                                      ggTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAAct                                                                                                                       >  1:1209902/1‑61 (MQ=255)
                                                                                      ggTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAAct                                                                                                                       <  2:1209902/61‑1 (MQ=255)
                                                                                      ggTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGaccac                                                                                                 <  1:1931739/83‑1 (MQ=255)
                                                                                      ggTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGaccac                                                                                                 >  2:1931739/1‑83 (MQ=255)
                                                                                      ggTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGa                                                                    >  2:370596/1‑112 (MQ=255)
                                                                                      ggTTACTCTACGGACGTGGGACCGAGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGa                                                                    <  1:370596/112‑1 (MQ=255)
                                                                                        ttACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCgctg                                       <  2:608149/139‑1 (MQ=255)
                                                                                        ttACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCgctg                                       >  2:609494/1‑139 (MQ=255)
                                                                                         tACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTgg                                                                                                                              >  2:1485662/1‑51 (MQ=255)
                                                                                         tACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTgg                                                                                                                              <  1:1485662/51‑1 (MQ=255)
                                                                                          aCTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCgctgct                                     <  2:551551/139‑1 (MQ=255)
                                                                                          aCTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCgctgct                                     <  1:874087/139‑1 (MQ=255)
                                                                                                cGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTgaagaa                               <  2:1137543/139‑1 (MQ=255)
                                                                                                   aCGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGaa                                                                                                                         >  1:71645/1‑46 (MQ=255)
                                                                                                   aCGTGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGaa                                                                                                                         <  2:71645/46‑1 (MQ=255)
                                                                                                      tGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACGAGt                                                                                             <  1:228440/71‑1 (MQ=255)
                                                                                                      tGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGt                                                                                             >  2:228440/1‑71 (MQ=255)
                                                                                                      tGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATaa                                                 <  2:1831330/115‑1 (MQ=255)
                                                                                                      tGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATaa                                                 >  1:1831330/1‑115 (MQ=255)
                                                                                                      tGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCgctg                                       >  2:527217/1‑125 (MQ=255)
                                                                                                      tGGGACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCgctg                                       <  1:527217/125‑1 (MQ=255)
                                                                                                       gggACCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTGAAGAATCCACTg                        >  1:989063/1‑139 (MQ=255)
                                                                                                          aCCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTGAAGAATCCACTGGaa                     <  2:989063/139‑1 (MQ=255)
                                                                                                          aCCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTGAAGAATCCACTGGaa                     <  2:282987/139‑1 (MQ=255)
                                                                                                          aCCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTGAAGAATCCACTGGaa                     <  1:463529/139‑1 (MQ=255)
                                                                                                          aCCGCGCGTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTGAAGAATCCACTGGaa                     <  1:57285/139‑1 (MQ=255)
                                                                                                             gcgcgTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTa                                                                                                                   >  1:1230864/1‑42 (MQ=255)
                                                                                                             gcgcgTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTa                                                                                                                   <  2:1230864/42‑1 (MQ=255)
                                                                                                             gcgcgTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTc                                                                                                              <  2:895041/47‑1 (MQ=255)
                                                                                                             gcgcgTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTc                                                                                                              >  1:895041/1‑47 (MQ=255)
                                                                                                               gcgTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTa                                                                                                                   <  1:225916/40‑1 (MQ=255)
                                                                                                               gcgTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTa                                                                                                                   >  2:225916/1‑40 (MQ=255)
                                                                                                               gcgTGAAAGCGACGTGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTGAAGAATCCACTGGAACAata                <  1:677685/139‑1 (MQ=255)
                                                                                                                             tGGAAAAGGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTGAAGAATCCACTGGAACAATATTATTTGGTGCAgg  <  1:609494/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
AATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTGAAGAATCCACTGGAACAATATTATTTGGTGCAGG  >  NC_000913/4443112‑4443375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑