Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,507,463	G→A	intergenic (+12/+3)	insO → / ← insI1	pseudogene, IS911 transposase B,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related/IS30 transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,507,463	0	G	A	100.0%	190.0 / NA	64	intergenic (+12/+3)	insO/insI1	pseudogene, IS911 transposase B,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related/IS30 transposase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/31);  total (33/31)

ACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAGATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGA  >  NC_000913/4507331‑4507592                                                                                                                                     |                                                                                                                                  acTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCaa                                                                                                                             <  2:1330585/139‑1 (MQ=255) acTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCaa                                                                                                                             <  2:1499796/139‑1 (MQ=255)  cTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAAt                                                                                                                            >  1:1207490/1‑139 (MQ=255)   tGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATc                                                                                                                           >  1:1016410/1‑139 (MQ=255)       tgatgaGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTc                                                                                                                       <  2:320133/139‑1 (MQ=255)            aGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGc                                                                                                                  >  1:1481406/1‑139 (MQ=255)                tCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAaca                                                                                                              <  2:1775045/139‑1 (MQ=255)                    ggCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACAcccc                                                                                                          <  2:1539438/139‑1 (MQ=255)                       aaaaCACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCttt                                                                                                       <  2:1207490/139‑1 (MQ=255)                         aaCACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCa                                                                                                     >  1:688146/1‑139 (MQ=255)                              cAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTAt                                                                                                >  2:1474082/1‑139 (MQ=255)                               aaaaGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCaa                                                                                                                             >  2:1785255/1‑109 (MQ=255)                               aaaaGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCaa                                                                                                                             <  1:1785255/109‑1 (MQ=255)                                                      aaCAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAg                                                                        >  2:1402151/1‑139 (MQ=255)                                                           aTCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCaa                                                                                                                             <  2:1764927/81‑1 (MQ=255)                                                           aTCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCaa                                                                                                                             >  1:1764927/1‑81 (MQ=255)                                                               aaaTACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGATACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTtct                                                               <  1:1474082/139‑1 (MQ=255)                                                                aaTACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCa                                                                                                                      <  2:422677/83‑1 (MQ=255)                                                                aaTACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCa                                                                                                                      >  1:422677/1‑83 (MQ=255)                                                                      tAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTc                                                                                                                       >  1:1123099/1‑76 (MQ=255)                                                                      tAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTc                                                                                                                       <  2:1123099/76‑1 (MQ=255)                                                                             aGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGgtg                                                                                     >  2:1147278/1‑103 (MQ=255)                                                                             aGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGgtg                                                                                     >  2:935429/1‑103 (MQ=255)                                                                             aGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGgtg                                                                                     <  1:935429/103‑1 (MQ=255)                                                                             aGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGgtg                                                                                     <  1:1147278/103‑1 (MQ=255)                                                                             aGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTa                                                 >  2:959456/1‑139 (MQ=255)                                                                             aGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTa                                                 >  1:1298125/1‑139 (MQ=255)                                                                               gAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAaca                                                                                                              >  2:1995641/1‑76 (MQ=255)                                                                               gAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAaca                                                                                                              <  1:1995641/76‑1 (MQ=255)                                                                                   aaGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTAtc                                                                                               <  1:1466104/87‑1 (MQ=255)                                                                                   aaGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTAtc                                                                                               >  2:1466104/1‑87 (MQ=255)                                                                                    aGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCa                                                                                                                      >  2:1696611/1‑63 (MQ=255)                                                                                    aGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCa                                                                                                                      <  1:1696611/63‑1 (MQ=255)                                                                                    aGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTAt                                                                                                <  2:373543/85‑1 (MQ=255)                                                                                    aGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTAt                                                                                                >  1:373543/1‑85 (MQ=255)                                                                                       tACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGagcagc                                       >  2:1116783/1‑139 (MQ=255)                                                                                       tACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGagcagc                                       >  2:560020/1‑139 (MQ=255)                                                                                          aaCGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAAc                                    <  1:1116783/139‑1 (MQ=255)                                                                                               aTTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGAt                               <  1:302938/139‑1 (MQ=255)                                                                                               aTTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGAt                               >  1:1016132/1‑139 (MQ=255)                                                                                               aTTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGAt                               >  2:302938/1‑139 (MQ=255)                                                                                                        gagaATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTc                                                          >  1:655407/1‑103 (MQ=255)                                                                                                        gagaATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTc                                                          <  2:655407/103‑1 (MQ=255)                                                                                                        gagaATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCAt                      <  2:1481406/139‑1 (MQ=255)                                                                                                          gaATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGt                    <  2:1298125/139‑1 (MQ=255)                                                                                                           aaTGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGtt                   >  1:458980/1‑139 (MQ=255)                                                                                                            aTGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTGTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTg                  <  1:1402151/139‑1 (MQ=255)                                                                                                               aaaTATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGt               <  2:1291429/139‑1 (MQ=255)                                                                                                                         aaGGCTACTGTTAATTCAAGCTGTCAATGCAACACCCATTTCATTTCTCACTTTCGGTGTTTTGGACTTAAGTGTCTTCCTCGGTCTGTTGTTTAGCTGAGCAGCAGCCAGATCTAGTTCATGTTGAGTATATTGGGCa     >  1:442762/1‑139 (MQ=11)                                                                                                                            gCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATg                     >  1:1913097/1‑120 (MQ=17)                                                                                                                            gCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATg                     <  2:1913097/120‑1 (MQ=17)                                                                                                                            gCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGta              <  1:575419/127‑1 (MQ=18)                                                                                                                            gCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGta              >  2:575419/1‑127 (MQ=17)                                                                                                                            gCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGa  >  2:746195/1‑139 (MQ=17)                                                                                                                             cTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAAc                                    >  1:732262/1‑104 (MQ=17)                                                                                                                             cTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAAc                                    <  2:732262/104‑1 (MQ=17)                                                                                                                              tACTGTAAATTCAATCTGTCAATGCGACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGt                          <  1:1373572/113‑1 (MQ=9)                                                                                                                              tACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGa                                                                               >  2:1756621/1‑60 (MQ=255)                                                                                                                              tACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGa                                                                               <  1:1756621/60‑1 (MQ=16)                                                                                                                              tACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGt                          >  2:1373572/1‑113 (MQ=14)                                                                                                                              tACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGtata            >  1:849212/1‑127 (MQ=14)                                                                                                                              tACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGtata            <  2:849212/127‑1 (MQ=14)                                                                                                                               aCTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTc                                                          <  1:781023/80‑1 (MQ=14)                                                                                                                               aCTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTc                                                          >  2:781023/1‑80 (MQ=9)                                                                                                                                     |                                                                                                                                  ACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAGATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGA  >  NC_000913/4507331‑4507592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑