Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,538,785	C→T	*239* (TAG→TAA)	nanC ←	N‑acetylnuraminic acid outer membrane channel protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,538,785	0	C	T	100.0%	268.3 / NA	82	*239* (TAG→TAA)	nanC	N‑acetylnuraminic acid outer membrane channel protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/41);  total (41/41)

GTCGTTATCAATTGCTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTACTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTATT  >  NC_000913/4538649‑4538912                                                                                                                                         |                                                                                                                                gtCGTTATCAATTGCTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTctatta                                                                                                                               <  1:727484/139‑1 (MQ=255) gtCGTTATCAATTGCTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTctatta                                                                                                                               <  1:1898026/139‑1 (MQ=255)     ttATCAATTGCTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGt                                                                                                                           >  1:520867/1‑139 (MQ=255)     ttATCAATTGCTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGt                                                                                                                           >  2:1553099/1‑139 (MQ=255)          aaTTGCTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTaaa                                                                                                                      <  1:1481076/139‑1 (MQ=255)                  cGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGacacac                                                                                                              >  1:1838522/1‑139 (MQ=255)                          cTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGa                                                                                                      <  2:671308/139‑1 (MQ=255)                            tttAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATa                                                                                                    >  2:520743/1‑139 (MQ=255)                                     cacaGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATaa                                                                                                   >  1:822036/1‑131 (MQ=255)                                     cacaGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATaa                                                                                                   <  2:822036/131‑1 (MQ=255)                                     cacaGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTc                                                                                           >  1:634151/1‑139 (MQ=255)                                     cacaGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTc                                                                                           >  2:1329930/1‑139 (MQ=255)                                               tCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCaa                                                                                                           >  2:1756034/1‑113 (MQ=255)                                               tCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCaa                                                                                                           <  1:1756034/113‑1 (MQ=255)                                                    tAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATctct                                                                            >  2:863820/1‑139 (MQ=255)                                                     aaTACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                           <  2:271015/139‑1 (MQ=255)                                                     aaTACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                           <  1:1329930/139‑1 (MQ=255)                                                            aCGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGaca                                                                                                                 >  2:170123/1‑94 (MQ=255)                                                            aCGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGaca                                                                                                                 <  1:170123/94‑1 (MQ=255)                                                            aCGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGt                                                                    >  1:1376418/1‑139 (MQ=255)                                                              gcgTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTaa                                                                  >  1:962999/1‑139 (MQ=255)                                                                 tttGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTAGTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATGACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAaca                                                               <  2:1376418/139‑1 (MQ=255)                                                                      ggCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTg                                                          <  2:520867/139‑1 (MQ=255)                                                                      ggCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTg                                                          <  1:863820/139‑1 (MQ=255)                                                                        cAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGAc                                                        >  2:63973/1‑139 (MQ=255)                                                                               aGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTAACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAg                                                 <  1:575637/139‑1 (MQ=255)                                                                                gCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                                <  1:560837/139‑1 (MQ=255)                                                                                    tcatGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTa                                                                                                                        >  2:540774/1‑63 (MQ=255)                                                                                    tcatGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTa                                                                                                                        <  1:540774/63‑1 (MQ=255)                                                                                    tcatGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGt                                            <  1:1553099/139‑1 (MQ=255)                                                                                    tcatGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGt                                            <  2:866766/139‑1 (MQ=255)                                                                                     catGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTaaa                                                                 <  1:122875/117‑1 (MQ=255)                                                                                     catGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                                >  1:1704214/1‑134 (MQ=255)                                                                                     catGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTc                                           <  1:520743/139‑1 (MQ=255)                                                                                     catGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGCaaa                                                                 >  2:122875/1‑117 (MQ=255)                                                                                     catGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAACTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                                <  2:1704214/134‑1 (MQ=255)                                                                                             cAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCg                                                                                         <  2:1770267/85‑1 (MQ=255)                                                                                             cAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCg                                                                                         >  1:1770267/1‑85 (MQ=255)                                                                                               aGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAAt                                                                                   >  2:940142/1‑89 (MQ=255)                                                                                               aGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAAt                                                                                   <  1:940142/89‑1 (MQ=255)                                                                                               aGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGAc                                                        >  2:217892/1‑116 (MQ=255)                                                                                               aGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGAc                                                        <  1:217892/116‑1 (MQ=255)                                                                                                gcgcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTcc                                                                                          >  2:1812751/1‑81 (MQ=255)                                                                                                gcgcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTcc                                                                                          <  1:1812751/81‑1 (MQ=255)                                                                                                 cgcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCtatgta                               <  2:634151/139‑1 (MQ=255)                                                                                                 cgcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCtatgta                               <  1:63973/139‑1 (MQ=255)                                                                                                  gcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                           <  2:1056584/94‑1 (MQ=255)                                                                                                  gcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                           >  1:1056584/1‑94 (MQ=255)                                                                                                  gcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTaaa                                                                 >  2:1341044/1‑104 (MQ=255)                                                                                                  gcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTaaa                                                                 <  1:1341044/104‑1 (MQ=255)                                                                                                   ccGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTAt                                                                                <  2:2041195/88‑1 (MQ=255)                                                                                                   ccGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTAt                                                                                >  1:2041195/1‑88 (MQ=255)                                                                                                              ttATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGt                                                                       >  1:4007/1‑86 (MQ=255)                                                                                                              ttATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGt                                                                       <  2:4007/86‑1 (MQ=255)                                                                                                              ttATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCg                  >  2:199040/1‑139 (MQ=255)                                                                                                                aTTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCggg                >  1:249577/1‑139 (MQ=255)                                                                                                                aTTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCggg                >  1:63549/1‑139 (MQ=255)                                                                                                                  tCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCg              <  2:962999/139‑1 (MQ=255)                                                                                                                      tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTAtct                                                                              >  1:812694/1‑71 (MQ=255)                                                                                                                      tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTAtct                                                                              <  2:812694/71‑1 (MQ=255)                                                                                                                      tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCg                                                                        >  2:1706706/1‑77 (MQ=255)                                                                                                                      tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCg                                                                        <  1:1706706/77‑1 (MQ=255)                                                                                                                      tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTg                                                          >  2:1593114/1‑91 (MQ=255)                                                                                                                      tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTg                                                          <  2:1529999/91‑1 (MQ=255)                                                                                                                      tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTg                                                          >  1:1529999/1‑91 (MQ=255)                                                                                                                      tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTg                                                          <  1:1593114/91‑1 (MQ=255)                                                                                                                      tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                                >  1:2047462/1‑101 (MQ=255)                                                                                                                      tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                                <  2:2047462/101‑1 (MQ=255)                                                                                                                      tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTc                                           >  1:654811/1‑106 (MQ=255)                                                                                                                      tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTc                                           <  2:654811/106‑1 (MQ=255)                                                                                                                      tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAata                     <  1:860236/128‑1 (MQ=255)                                                                                                                      tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAata                     >  2:860236/1‑128 (MQ=255)                                                                                                                      tgtgACTGTCTCCTGTCTATTACAGTTTAAATGAAACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGAGCGTCat          >  2:831418/1‑139 (MQ=255)                                                                                                                       tttaCTGTCTCCTGTCTATTACAGTTTGAATGCCACACCCATCCGCTAACTGTTTTCCGTTTAATTATCTCTGCCGTTGTATCCACCCTGACGGTCATCGTAGTCATATTCTATGTATGGTGTAATATCGGGCGTCata         <  1:831418/136‑1 (MQ=255)                                                                                                                           cTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCtatgta                               <  1:549436/113‑1 (MQ=255)                                                                                                                           cTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCtatgta                               >  2:549436/1‑113 (MQ=255)                                                                                                                              tctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTAtt  >  2:742980/1‑139 (MQ=255)                                                                                                                              tctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCCCTCCCGTTTTAAACACCCTGCCGGCCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTAATATGGTAtt  >  2:1086476/1‑139 (MQ=255)                                                                                                                                tcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTc                                           >  2:831170/1‑96 (MQ=255)                                                                                                                                tcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTc                                           <  1:831170/96‑1 (MQ=255)                                                                                                                                    gTCTATTACAGTTTAAATGACACACCAATGTGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTg                                                          <  1:187091/77‑1 (MQ=255)                                                                                                                                    gTCTATTACAGTTTAAATGACACACCAATGTGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTg                                                          >  2:187091/1‑77 (MQ=255)                                                                                                                                    gTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCggg                <  1:939511/119‑1 (MQ=255)                                                                                                                                    gTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCggg                >  2:939511/1‑119 (MQ=255)                                                                                                                                         |                                                                                                                                GTCGTTATCAATTGCTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTACTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTCATATGGTATT  >  NC_000913/4538649‑4538912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑