Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,585,322 C→T T480T (ACG→ACA hsdR ← endonuclease R Type I restriction enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,585,3220CT97.8% 288.6 / ‑2.2 92T480T (ACG→ACAhsdRendonuclease R Type I restriction enzyme
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (44/46);  total (45/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

TGTTGAAGGTGTCGCCGTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATC  >  NC_000913/4585186‑4585456
                                                                                                                                        |                                                                                                                                      
tGTTGAAGGTGTCGCCGTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGt                                                                                                                                      >  1:908635/1‑139 (MQ=255)
 gTTGAAGGTGTCGCCGTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGAGCAGGCGGAACATCATGGCGATTGCTGTa                                                                                                                                     <  1:1172797/139‑1 (MQ=255)
      aGGTGTCGCCGTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGt                                                                                                                                <  1:1768755/139‑1 (MQ=255)
         tgtCGCCGTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGtttt                                                                                                                             >  2:1939245/1‑139 (MQ=255)
            cGCCGTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTtacc                                                                                                                          >  2:482351/1‑139 (MQ=255)
                        gcgTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgc                                                                                                              >  1:1268358/1‑139 (MQ=255)
                           tATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGACCGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgccat                                                                                                           >  1:14941/1‑139 (MQ=255)
                           tATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgccat                                                                                                           >  2:1714273/1‑139 (MQ=255)
                           tATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgccat                                                                                                           >  1:812011/1‑139 (MQ=255)
                           tATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgccat                                                                                                           >  2:680539/1‑139 (MQ=255)
                            aTCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgccatc                                                                                                          <  1:482351/139‑1 (MQ=255)
                               ttCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgccatcgcc                                                                                                       <  2:1799728/139‑1 (MQ=255)
                                 cAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgccatc                                                                                                          >  2:1675852/1‑134 (MQ=255)
                                 cAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgccatc                                                                                                          >  2:370611/1‑134 (MQ=255)
                                 cAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgccatc                                                                                                          <  1:1675852/134‑1 (MQ=255)
                                 cAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgccatc                                                                                                          <  1:370611/134‑1 (MQ=255)
                                  aaaCGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagc                                                                                                    <  1:680539/139‑1 (MQ=255)
                                       cgcCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgcc                                                                                                             >  2:1947707/1‑125 (MQ=255)
                                       cgcCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgcc                                                                                                             <  1:1947707/125‑1 (MQ=255)
                                       cgcCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGAt                                                                                               <  2:908635/139‑1 (MQ=255)
                                        gcCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGAtc                                                                                              >  2:1160687/1‑139 (MQ=255)
                                              cgcCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGATTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCttgt                                                                                        <  1:1718431/139‑1 (MQ=255)
                                                 cTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGc                                                                                     <  1:1276936/139‑1 (MQ=255)
                                                    ttCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGt                                                                                                                                >  2:618439/1‑93 (MQ=255)
                                                    ttCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGt                                                                                                                                <  1:618439/93‑1 (MQ=255)
                                                    ttCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGcccc                                                                                  >  1:2035539/1‑139 (MQ=255)
                                                             agaACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGAtt                                                                         <  1:1686149/139‑1 (MQ=255)
                                                                      gTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGAtc                                                                                              >  1:1829425/1‑109 (MQ=255)
                                                                      gTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGAtc                                                                                              <  2:1829425/109‑1 (MQ=255)
                                                                       tCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGCACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCaa                                                               >  1:1198727/1‑139 (MQ=255)
                                                                          aCAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACcg                                                            <  2:14941/139‑1 (MQ=255)
                                                                            aaGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACcgcg                                                          <  2:2035539/139‑1 (MQ=255)
                                                                              ggAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACcgcgcg                                                        >  1:517371/1‑139 (MQ=255)
                                                                              ggAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACcgcgcg                                                        >  2:754412/1‑139 (MQ=255)
                                                                                    gaATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCt                                                                                 <  2:364177/108‑1 (MQ=255)
                                                                                    gaATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCt                                                                                 >  1:364177/1‑108 (MQ=255)
                                                                                     aaTGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACgg                                                   >  2:743219/1‑137 (MQ=255)
                                                                                     aaTGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACgg                                                   <  1:743219/137‑1 (MQ=255)
                                                                                     aaTGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCa                                                 <  2:1268358/139‑1 (MQ=255)
                                                                                     aaTGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCa                                                 >  1:1763297/1‑139 (MQ=255)
                                                                                       tGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagc                                                                                                    >  2:700460/1‑86 (MQ=255)
                                                                                       tGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagc                                                                                                    <  1:700460/86‑1 (MQ=255)
                                                                                       tGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATc                                               <  2:812011/139‑1 (MQ=255)
                                                                                       tGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATc                                               <  2:517371/139‑1 (MQ=255)
                                                                                        gCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGa                                                      <  2:1432090/131‑1 (MQ=255)
                                                                                        gCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGa                                                      >  1:1432090/1‑131 (MQ=255)
                                                                                        gCGTTTAAAACGCTGGGACTGGATCAGACGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCt                                              >  2:2039975/1‑139 (MQ=255)
                                                                                         cGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATc                                               <  2:2033139/137‑1 (MQ=255)
                                                                                         cGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATc                                               >  1:2033139/1‑137 (MQ=255)
                                                                                         cGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCtt                                             >  1:156012/1‑139 (MQ=255)
                                                                                          gTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGt                                                                                                                                <  2:351852/55‑1 (MQ=255)
                                                                                          gTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGt                                                                                                                                >  1:351852/1‑55 (MQ=255)
                                                                                          gTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCttgtt                                                                                       >  1:845440/1‑96 (MQ=255)
                                                                                          gTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCttgtt                                                                                       <  2:845440/96‑1 (MQ=255)
                                                                                          gTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTc                                            <  1:1714273/139‑1 (MQ=255)
                                                                                           tttAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTcc                                           <  1:1160687/139‑1 (MQ=255)
                                                                                            ttAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCt                                          <  2:1198727/139‑1 (MQ=255)
                                                                                               aaaCGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCtt                                             <  2:2055549/133‑1 (MQ=255)
                                                                                               aaaCGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCtt                                             >  1:2055549/1‑133 (MQ=255)
                                                                                                     tGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAAc                                 >  2:841817/1‑139 (MQ=255)
                                                                                                          cTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAgg                            <  1:1939245/139‑1 (MQ=255)
                                                                                                           tGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGc                           <  2:1763297/139‑1 (MQ=255)
                                                                                                             gATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgccatcg                                                                                                         >  1:1455567/1‑59 (MQ=255)
                                                                                                             gATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgccatcg                                                                                                         <  2:1455567/59‑1 (MQ=255)
                                                                                                             gATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGc                                                                                     <  2:878849/79‑1 (MQ=255)
                                                                                                             gATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGc                                                                                     >  1:878849/1‑79 (MQ=255)
                                                                                                                  ggCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAgctc                    >  2:1795667/1‑139 (MQ=255)
                                                                                                                     ggAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAgctcgct                 <  1:2039975/139‑1 (MQ=255)
                                                                                                                       aaCATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgccatcgcca                                                                                                      <  1:623270/52‑1 (MQ=255)
                                                                                                                       aaCATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGtcgccatcgcca                                                                                                      >  2:623270/1‑52 (MQ=255)
                                                                                                                       aaCATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGAtt                                                                         >  2:811776/1‑81 (MQ=255)
                                                                                                                       aaCATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGAtt                                                                         <  1:811776/81‑1 (MQ=255)
                                                                                                                       aaCATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGa                                                      <  1:640776/100‑1 (MQ=255)
                                                                                                                       aaCATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGa                                                      >  2:640776/1‑100 (MQ=255)
                                                                                                                       aaCATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACg                                <  1:1070951/122‑1 (MQ=255)
                                                                                                                       aaCATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACg                                >  2:1070951/1‑122 (MQ=255)
                                                                                                                        aCATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCt                                          >  2:168577/1‑111 (MQ=255)
                                                                                                                        aCATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCt                                          <  1:168577/111‑1 (MQ=255)
                                                                                                                            catGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCa          <  1:1596575/139‑1 (MQ=255)
                                                                                                                            catGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAgctcgct                 >  1:1528046/1‑132 (MQ=255)
                                                                                                                            catGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAgctcgct                 <  2:1528046/132‑1 (MQ=255)
                                                                                                                                 cGATTGCTGTACGGGTTTTACCGGTACCGGtcgccatcgcca                                                                                                      <  1:1218952/42‑1 (MQ=255)
                                                                                                                                 cGATTGCTGTACGGGTTTTACCGGTACCGGtcgccatcgcca                                                                                                      >  2:1218952/1‑42 (MQ=255)
                                                                                                                                 cGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGAtt                                                                         <  2:1222736/71‑1 (MQ=255)
                                                                                                                                 cGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCt                                          <  2:702885/102‑1 (MQ=255)
                                                                                                                                 cGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCt                                          >  1:702885/1‑102 (MQ=255)
                                                                                                                                 cGATTGCTGTACGGGCTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGAtt                                                                         >  1:1222736/1‑71 (MQ=255)
                                                                                                                                    ttGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCttgtt                                                                                       <  2:561425/54‑1 (MQ=255)
                                                                                                                                    ttGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCttgtt                                                                                       >  1:561425/1‑54 (MQ=255)
                                                                                                                                    ttGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGTCTTTTCAACCGCGCGGACg                                                    >  1:526659/1‑89 (MQ=255)
                                                                                                                                    ttGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGTCTTTTCAACCGCGCGGACg                                                    <  2:526659/89‑1 (MQ=255)
                                                                                                                                    ttGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATc  >  2:1046157/1‑139 (MQ=255)
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TGTTGAAGGTGTCGCCGTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATC  >  NC_000913/4585186‑4585456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: