Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 406,492 T→C F30L (TTT→CTT)  aroL → shikimate kinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913406,4920TC100.0% 361.5 / NA 100F30L (TTT→CTT) aroLshikimate kinase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/49);  total (51/49)

Only 100 of 101 total aligned reads displayed.

ATGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTGGAAGCGGTAACTGCGCCATCCACCGTTA  >  NC_000913/406358‑406627
                                                                                                                                      |                                                                                                                                       
aTGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGCTGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGACCTTGCCGATTCGCTTAACCGTCGGCTTGt                                                                                                                                     <  1:1903214/139‑1 (MQ=255)
aTGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGt                                                                                                                                     >  2:1254063/1‑139 (MQ=255)
aTGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGt                                                                                                                                     >  1:608536/1‑139 (MQ=255)
aTGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGt                                                                                                                                     <  1:1254063/139‑1 (MQ=255)
aTGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGt                                                                                                                                     >  2:1611126/1‑139 (MQ=255)
aTGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGt                                                                                                                                     >  1:1925021/1‑139 (MQ=255)
aTGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGt                                                                                                                                     >  2:1903214/1‑139 (MQ=255)
  gACACCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCg                                                                                                                                   >  2:238174/1‑139 (MQ=255)
    cacCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGAt                                                                                                                                 <  2:752113/139‑1 (MQ=255)
     acCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATa                                                                                                                                <  2:1925021/139‑1 (MQ=255)
     acCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATa                                                                                                                                <  1:710375/139‑1 (MQ=255)
       cGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATAcc                                                                                                                              >  2:253291/1‑139 (MQ=255)
        ggCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCg                                                                                                                             <  2:2023271/139‑1 (MQ=255)
         gCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGCCGGAATGGCCGTTGCCGATTCGCTTAACCGTCGGCTTGTCGATcccta                                                                                                                            >  1:1309267/1‑137 (MQ=255)
          cTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGAt                                                                                                                           <  2:589071/139‑1 (MQ=255)
               gccgcATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTg                                                                                                                      <  1:1611126/139‑1 (MQ=255)
                    aTTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGc                                                                                                                 <  2:608536/139‑1 (MQ=255)
                             tATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGc                                                                                                        >  1:1192352/1‑139 (MQ=255)
                              aTTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCt                                                                                                       <  1:238174/139‑1 (MQ=255)
                                       aaCCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGAt                                                                                                                           <  2:116903/110‑1 (MQ=255)
                                       aaCCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGAt                                                                                                                           >  1:116903/1‑110 (MQ=255)
                                       aaCCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGAc                                                                                              >  2:1867802/1‑139 (MQ=255)
                                         cccACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTTGATACCGATCAg                                                                                                                        >  2:843875/1‑111 (MQ=255)
                                         cccACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTTGATACCGATCAg                                                                                                                        <  1:843875/111‑1 (MQ=255)
                                         cccACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCa                                                                                                                         <  1:446034/110‑1 (MQ=255)
                                         cccACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCa                                                                                                                         >  2:446034/1‑110 (MQ=255)
                                         cccACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACgg                                                                                            >  1:1530556/1‑139 (MQ=255)
                                         cccACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACg                                                                                             <  1:884385/138‑1 (MQ=255)
                                         cccACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACg                                                                                             >  2:884385/1‑138 (MQ=255)
                                            aCGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTcg                                                                                         >  2:1564139/1‑139 (MQ=255)
                                                      aaCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGAtcgt                                                                               <  1:519854/139‑1 (MQ=255)
                                                        ccTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATa                                                                                                                                >  2:1405304/1‑88 (MQ=255)
                                                        ccTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATa                                                                                                                                <  1:1405304/88‑1 (MQ=255)
                                                        ccTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGAtcgtcg                                                                             <  1:1564139/139‑1 (MQ=255)
                                                        ccTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGAtcgtcg                                                                             >  2:2040401/1‑139 (MQ=255)
                                                           ctTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGaaa                                                                          <  1:1593496/139‑1 (MQ=255)
                                                           ctTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGaaa                                                                          >  2:2046904/1‑139 (MQ=255)
                                                            tttttCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAg                                                                         >  2:1619118/1‑139 (MQ=255)
                                                                  tGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGAtcgtcg                                                                             >  1:325069/1‑129 (MQ=255)
                                                                  tGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGAtcgtcg                                                                             <  2:325069/129‑1 (MQ=255)
                                                                   gATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACg                                                                                             <  2:1352558/112‑1 (MQ=255)
                                                                   gATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCACTGGTTGCAATCACAGCTCAATATGACg                                                                                             >  1:1352558/1‑112 (MQ=255)
                                                                    aTCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAg                                                                                                                        >  1:737818/1‑84 (MQ=255)
                                                                    aTCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAg                                                                                                                        <  2:737818/84‑1 (MQ=255)
                                                                    aTCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATca                                                                                                            >  2:1977901/1‑96 (MQ=255)
                                                                    aTCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATca                                                                                                            <  1:1977901/96‑1 (MQ=255)
                                                                      cGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATca                                                                                                            >  2:929747/1‑94 (MQ=255)
                                                                      cGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATca                                                                                                            <  1:929747/94‑1 (MQ=255)
                                                                         gCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGc                                                                                                        >  2:770744/1‑95 (MQ=255)
                                                                         gCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGc                                                                                                        <  1:770744/95‑1 (MQ=255)
                                                                         gCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCg                                                            <  1:1867802/139‑1 (MQ=255)
                                                                          ccTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCgg                                                           <  2:1192352/139‑1 (MQ=255)
                                                                              ggggCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTcgcg                                                                                       >  1:1601971/1‑107 (MQ=255)
                                                                              ggggCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTcgcg                                                                                       <  2:1601971/107‑1 (MQ=255)
                                                                                   tgtgGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAAt                                                                                                              <  2:1721446/79‑1 (MQ=255)
                                                                                   tgtgGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAAt                                                                                                              >  1:1721446/1‑79 (MQ=255)
                                                                                   tgtgGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCgg                                                                                      >  2:1655001/1‑103 (MQ=255)
                                                                                   tgtgGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCgg                                                                                      <  1:1655001/103‑1 (MQ=255)
                                                                                    gtgGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCgcgc                                                 <  1:253291/139‑1 (MQ=255)
                                                                                     tgGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGAtcgt                                                                               >  1:1343975/1‑108 (MQ=255)
                                                                                     tgGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGAtcgt                                                                               <  2:1343975/108‑1 (MQ=255)
                                                                                     tgGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGcc                                                >  1:1725113/1‑139 (MQ=255)
                                                                                       gTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCag                                              >  1:1297277/1‑139 (MQ=255)
                                                                                               acGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGa                                                                                                                            >  2:1209462/1‑53 (MQ=255)
                                                                                               acGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGa                                                                                                                            <  1:1209462/53‑1 (MQ=255)
                                                                                               acGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAAcgg                                      >  2:1704677/1‑139 (MQ=255)
                                                                                               acGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAAcgg                                      <  1:1619118/139‑1 (MQ=255)
                                                                                                cGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATa                                                                                                                                >  2:1147195/1‑48 (MQ=255)
                                                                                                cGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATa                                                                                                                                <  1:1147195/48‑1 (MQ=255)
                                                                                                cGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGAt                                                                                                                           <  1:2047635/53‑1 (MQ=255)
                                                                                                cGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGAt                                                                                                                           >  2:2047635/1‑53 (MQ=255)
                                                                                                 ggTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAAcggcg                                    >  1:102433/1‑139 (MQ=255)
                                                                                                  gTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTcg                                                                                         <  2:948920/85‑1 (MQ=255)
                                                                                                  gTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTcg                                                                                         >  1:948920/1‑85 (MQ=255)
                                                                                                  gTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGaa                                                                           >  2:1874920/1‑99 (MQ=255)
                                                                                                  gTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGaa                                                                           <  1:1874920/99‑1 (MQ=255)
                                                                                                   tCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGAttt                                                      >  2:218290/1‑119 (MQ=255)
                                                                                                   tCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGAttt                                                      <  1:218290/119‑1 (MQ=255)
                                                                                                    cGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGgcggcg                                 <  2:102433/139‑1 (MQ=255)
                                                                                                       aaTGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTg                              <  1:1704677/139‑1 (MQ=255)
                                                                                                       aaTGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTg                              <  1:988426/139‑1 (MQ=255)
                                                                                                                tGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTg                                                                                                                      >  1:126029/1‑42 (MQ=255)
                                                                                                                tGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTg                                                                                                                      <  2:126029/42‑1 (MQ=255)
                                                                                                                tGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCgcgc                                                 <  2:633961/111‑1 (MQ=255)
                                                                                                                tGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCgcgc                                                 >  1:633961/1‑111 (MQ=255)
                                                                                                                 gCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGa                                                                                               >  1:513124/1‑64 (MQ=255)
                                                                                                                 gCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGa                                                                                               <  2:513124/64‑1 (MQ=255)
                                                                                                                 gCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGAtcgtcg                                                                             >  2:1399478/1‑82 (MQ=255)
                                                                                                                 gCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGAtcgtcg                                                                             <  1:1399478/82‑1 (MQ=255)
                                                                                                                 gCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGgcggcg                                 >  1:710441/1‑126 (MQ=255)
                                                                                                                 gCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGgcggcg                                 <  2:710441/126‑1 (MQ=255)
                                                                                                                 gCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTGGa                            >  2:1182790/1‑131 (MQ=255)
                                                                                                                 gCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTGGa                            <  1:1182790/131‑1 (MQ=255)
                                                                                                                     aTTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACg                                                                                             <  1:304413/62‑1 (MQ=255)
                                                                                                                     aTTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACg                                                                                             >  2:304413/1‑62 (MQ=255)
                                                                                                                     aTTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTGGAAGCGGTAACTgc                >  2:1802195/1‑139 (MQ=255)
                                                                                                                          cTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTGGAAGCGGTAACTGCGCCAt           >  1:1587366/1‑139 (MQ=255)
                                                                                                                                   cGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTcgc                                                                                        <  1:176019/53‑1 (MQ=255)
                                                                                                                                   cGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTcgc                                                                                        >  2:176019/1‑53 (MQ=255)
                                                                                                                                   cGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTACGCGCCAGAGAAACGGCGGCGCTGGAAGCGGTAACTGCGCCATCCACCGTTa  >  1:1509536/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                       
ATGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTGGAAGCGGTAACTGCGCCATCCACCGTTA  >  NC_000913/406358‑406627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: