Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,624,357	(T)8→7	coding (141/393 nt)	ydeI ←	hydrogen peroxide resistance OB fold protein, putative periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,624,350	0	T	.	100.0%	232.1 / NA	55	coding (148/393 nt)	ydeI	hydrogen peroxide resistance OB fold protein, putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (27/28);  total (27/28)

GCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATA  >  NC_000913/1624214‑1624478                                                                                                                                         |                                                                                                                                 gCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAttt                                                                                                                                >  1:9580/1‑139 (MQ=255)            aaCGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat                                                                                                                    <  1:996514/139‑1 (MQ=255)            aaCGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat                                                                                                                    <  2:964396/139‑1 (MQ=255)                  aTTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTc                                                                                                              >  2:210542/1‑139 (MQ=255)                   ttAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTcc                                                                                                             >  2:277863/1‑139 (MQ=255)                    tAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCcg                                                                                                            <  1:210542/139‑1 (MQ=255)                          cACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAg                                                                                                      <  1:625124/139‑1 (MQ=255)                          cACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAg                                                                                                      <  1:277863/139‑1 (MQ=255)                           aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGc                                                                                                     >  1:548393/1‑139 (MQ=255)                              gCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAt                                                                                                  <  2:9580/139‑1 (MQ=255)                               ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAtc                                                                                                 <  2:548393/139‑1 (MQ=255)                                tctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAtct                                                                                                <  2:379377/139‑1 (MQ=255)                                                       tCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGTTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTgg                                                                         >  2:593601/1‑139 (MQ=255)                                                         ctctCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGgcg                                                                       <  1:1076712/139‑1 (MQ=255)                                                              cTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTGTTGCATGAGGCGGCGGTGGCGCTGCa                                                                  >  1:748563/1‑139 (MQ=255)                                                                ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                <  1:1129359/139‑1 (MQ=255)                                                                 tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAttt                                                                                                                                >  2:747492/1‑75 (MQ=255)                                                                 tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAttt                                                                                                                                <  1:747492/75‑1 (MQ=255)                                                                 tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGcc                                                                                                    >  2:722121/1‑102 (MQ=255)                                                                 tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAt                                                                                                  >  1:146591/1‑104 (MQ=255)                                                                 tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAt                                                                                                  <  2:146591/104‑1 (MQ=255)                                                                 tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTCTGCGAAATCATCGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGcc                                                                                                    <  1:722121/102‑1 (MQ=255)                                                                    gagaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGt                                                            >  1:388801/1‑139 (MQ=255)                                                                     agaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGcc                                                                                                    <  2:584868/98‑1 (MQ=255)                                                                     agaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGcc                                                                                                    >  1:584868/1‑98 (MQ=255)                                                                      gaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGttt                                                          >  1:363899/1‑139 (MQ=255)                                                                        aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTa                                                        >  2:802220/1‑139 (MQ=255)                                                                         aTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaa                                                       >  2:682250/1‑139 (MQ=255)                                                                                 aCCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAtttttttgc                                                                                                                          <  1:849063/65‑3 (MQ=255)                                                                                 aCCGCGTAGTGAAACCGAAGCGCCATCGTGAATGGTTTTTGCGAAATCAACGGTCAtttttttgc                                                                                                                          >  2:849063/1‑63 (MQ=255)                                                                                        aGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATc                                        >  1:1034099/1‑139 (MQ=255)                                                                                            aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGTCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                    >  2:73252/1‑139 (MQ=255)                                                                                            aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                    >  2:643081/1‑139 (MQ=255)                                                                                            aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                    <  2:388801/139‑1 (MQ=255)                                                                                             aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGcc                                                                                                    <  1:661140/74‑1 (MQ=255)                                                                                             aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCCATAGcc                                                                                                    >  2:661140/1‑74 (MQ=255)                                                                                              aCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCaa                                  <  2:363899/139‑1 (MQ=255)                                                                                                  aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCa                                                                                                   >  2:1086050/1‑70 (MQ=255)                                                                                                  aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCa                                                                                                   <  1:1086050/70‑1 (MQ=255)                                                                                                  aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCa                                                                  >  1:382574/1‑103 (MQ=255)                                                                                                  aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCa                                                                  <  2:382574/103‑1 (MQ=255)                                                                                                   aGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                              <  1:1059656/138‑1 (MQ=255)                                                                                                   aGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                              >  2:1059656/1‑138 (MQ=255)                                                                                                    gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCt                                                                     >  2:573897/1‑98 (MQ=255)                                                                                                    gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCt                                                                     <  1:573897/98‑1 (MQ=255)                                                                                                    gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                >  2:551721/1‑103 (MQ=255)                                                                                                    gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                <  1:551721/103‑1 (MQ=255)                                                                                                                    tttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGtt                                                                                                               >  2:969617/1‑40 (MQ=37)                                                                                                                    tttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGtt                                                                                                               <  1:969617/40‑1 (MQ=37)                                                                                                                    tttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa            <  1:802220/139‑1 (MQ=255)                                                                                                                    tttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa            <  1:643081/139‑1 (MQ=255)                                                                                                                        tGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTa                                                                                            >  2:236370/1‑55 (MQ=255)                                                                                                                        tGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTa                                                                                            <  1:236370/55‑1 (MQ=255)                                                                                                                           gAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGcc                                                 <  1:487421/95‑1 (MQ=255)                                                                                                                           gAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGcc                                                 >  2:487421/1‑95 (MQ=255)                                                                                                                              aTCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAtc                                                                                                 >  2:660651/1‑44 (MQ=38)                                                                                                                              aTCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAtc                                                                                                 <  1:660651/44‑1 (MQ=38)                                                                                                                              aTCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATa  >  1:1131704/1‑139 (MQ=255)                                                                                                                                  aCGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                <  1:845183/73‑1 (MQ=255)                                                                                                                                  aCGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                >  2:845183/1‑73 (MQ=255)                                                                                                                                         |                                                                                                                                 GCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATA  >  NC_000913/1624214‑1624478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑