Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,685,5370C.100.0% 154.0 / NA 37coding (1052/1404 nt)fumCfumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (20/17);  total (20/17)

CTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTC  >  NC_000913/1685401‑1685667
                                                                                                                                        |                                                                                                                                  
cTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGccc                                                                                                                                  >  2:896665/1‑139 (MQ=255)
cTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGccc                                                                                                                                  <  2:845264/139‑1 (MQ=255)
               aaTACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCa                                                                                                                  <  1:896665/139‑1 (MQ=255)
                         gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCa                                                                                                                  <  2:684391/129‑1 (MQ=255)
                         gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTccc                                                                                                        >  2:569842/1‑139 (MQ=255)
                         gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTccc                                                                                                        >  2:115790/1‑139 (MQ=255)
                         gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTAGACGGAAGACGTTCAGTTCAAAGTTACAGGAAGCGCCCCCATGTTGATCGCCa                                                                                                                  >  1:684391/1‑129 (MQ=255)
                          cgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTcccc                                                                                                       >  2:832770/1‑139 (MQ=255)
                          cgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTcccc                                                                                                       >  1:512518/1‑139 (MQ=255)
                           gcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGcc                                                                                                                                   <  2:229084/111‑1 (MQ=255)
                           gcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGCTACGGGAAGCGcc                                                                                                                                   >  1:229084/1‑111 (MQ=255)
                                      tAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                           <  2:354370/139‑1 (MQ=255)
                                      tAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                           <  1:832770/139‑1 (MQ=255)
                                         aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                        >  1:1065839/1‑139 (MQ=255)
                                         aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                        >  1:1050366/1‑139 (MQ=255)
                                         aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                           <  1:104787/136‑1 (MQ=255)
                                         aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                           >  2:104787/1‑136 (MQ=255)
                                             ttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                     >  1:303357/1‑138 (MQ=255)
                                             ttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                     <  2:303357/138‑1 (MQ=255)
                                              tCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCAt                                                                                   >  2:980444/1‑139 (MQ=255)
                                                        cTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                         <  1:569842/139‑1 (MQ=255)
                                                                     cACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                              <  1:1077764/121‑1 (MQ=255)
                                                                     cACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                              >  2:1077764/1‑121 (MQ=255)
                                                                                  aaaTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTccc                                               <  2:1050366/139‑1 (MQ=255)
                                                                                                cATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTgtgt                                                              <  1:882717/110‑1 (MQ=255)
                                                                                                cATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTgtgt                                                              >  2:882717/1‑110 (MQ=255)
                                                                                                       ctgAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCgg                          >  1:1021975/3‑139 (MQ=255)
                                                                                                       cGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCa                                                                                                   >  1:913575/1‑66 (MQ=255)
                                                                                                       cGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCa                                                                                                   <  2:913575/66‑1 (MQ=255)
                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTcccc                                                                                                       <  1:808878/59‑1 (MQ=255)
                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTcccc                                                                                                       >  2:808878/1‑59 (MQ=255)
                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                       >  1:768731/1‑139 (MQ=255)
                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                       >  2:1004775/1‑139 (MQ=255)
                                                                                                               gttcagttcaAAGTTACCGGCAGCGCCCCCATGTTGATCGCCACGTCGTTccc                                                                                                        <  2:666594/53‑1 (MQ=38)
                                                                                                               gttcagttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTccc                                                                                                        >  1:666594/1‑53 (MQ=255)
                                                                                                                 tcagttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTcc                <  2:909398/139‑1 (MQ=255)
                                                                                                                  cagttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCg               <  1:980444/139‑1 (MQ=255)
                                                                                                                          aGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTgag       <  2:1065839/139‑1 (MQ=255)
                                                                                                                               ccGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCAt                                         <  2:907216/100‑1 (MQ=255)
                                                                                                                               ccGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCAt                                         >  1:907216/1‑100 (MQ=255)
                                                                                                                               ccGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTc  >  2:14768/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                  
CTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTC  >  NC_000913/1685401‑1685667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: