Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,696,452 (A)5→4 intergenic (‑381/+10) uidA ← / ← uidR beta‑D‑glucuronidase/transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,696,4480A.100.0% 144.0 / NA 35intergenic (‑377/+14)uidA/uidRbeta‑D‑glucuronidase/transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (20/15);  total (20/15)

AACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTA  >  NC_000913/1696319‑1696580
                                                                                                                                 |                                                                                                                                    
aaCGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAagagaga                                                                                                                            >  2:1022407/1‑139 (MQ=255)
              aTGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGAt                                                                                                                  >  2:731626/1‑135 (MQ=255)
              aTGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGAt                                                                                                                  <  1:731626/135‑1 (MQ=255)
                          tACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGc                                                                                                  >  1:423833/1‑139 (MQ=255)
                                 aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagag                                                                                                                                <  2:869027/103‑4 (MQ=255)
                                 aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagag                                                                                                                                >  1:869027/1‑100 (MQ=255)
                                 aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcat                                                                                           >  2:411504/1‑139 (MQ=255)
                                              gAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATccc                                                                              <  1:1022407/139‑1 (MQ=255)
                                               aaTTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCt                                                                             >  2:533108/1‑139 (MQ=255)
                                                              ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATg                                                                                                                 >  2:1002442/1‑88 (MQ=255)
                                                              ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATg                                                                                                                 <  1:1002442/88‑1 (MQ=255)
                                                              ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCtt                                                              >  1:333867/1‑139 (MQ=255)
                                                                aaGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCa                                                            <  2:333867/139‑1 (MQ=255)
                                                                           ttGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               <  2:952267/62‑5 (MQ=255)
                                                                           ttGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                               >  1:952267/1‑58 (MQ=255)
                                                                                        ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    <  1:533108/139‑1 (MQ=255)
                                                                                             atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGa                                                                                                                   >  1:70311/1‑55 (MQ=255)
                                                                                             atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGa                                                                                                                   <  2:70311/55‑1 (MQ=255)
                                                                                               atGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    <  1:816721/132‑1 (MQ=255)
                                                                                               atGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    >  2:816721/1‑132 (MQ=255)
                                                                                                 gAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGt                           <  2:423833/139‑1 (MQ=255)
                                                                                                       cgGGCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCg                     <  1:382632/139‑1 (MQ=255)
                                                                                                        gggCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGc                                                                                                                <  1:765903/47‑1 (MQ=39)
                                                                                                        gggCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGc                                                                                                                >  2:765903/1‑47 (MQ=39)
                                                                                                        gggCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGc                                           >  2:500320/1‑116 (MQ=255)
                                                                                                        gggCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGc                                           <  1:500320/116‑1 (MQ=255)
                                                                                                        gggCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGt                    >  1:48819/1‑139 (MQ=255)
                                                                                                         ggCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTa                   >  1:767857/1‑139 (MQ=255)
                                                                                                          gCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATc                                  >  1:440246/1‑123 (MQ=255)
                                                                                                          gCACAGTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATc                                  <  2:440246/123‑1 (MQ=255)
                                                                                                               gTTATCACCAGCCCGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAg             <  2:767857/139‑1 (MQ=255)
                                                                                                                          cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcat                                                                                           <  1:890172/50‑1 (MQ=255)
                                                                                                                          cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcat                                                                                           >  2:890172/1‑50 (MQ=255)
                                                                                                                          cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  2:446057/1‑139 (MQ=255)
                                                                                                                          cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  2:666834/1‑139 (MQ=255)
                                                                                                                          cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  1:98018/1‑139 (MQ=255)
                                                                                                                          cccGCCGAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  2:891033/1‑139 (MQ=255)
                                                                                                                            cgccgAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCGGCTTCGATATCGGCCAGTacac                       <  2:287034/116‑1 (MQ=255)
                                                                                                                            cgccgAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCGGCTTCGATATCGGCCAGTacac                       >  1:287034/1‑116 (MQ=255)
                                                                                                                                 |                                                                                                                                    
AACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTA  >  NC_000913/1696319‑1696580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: