Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,850,810 (C)5→4 intergenic (+117/‑50) sppA → / → ansA protease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,850,8060C.100.0% 128.5 / NA 31intergenic (+113/‑54)sppA/ansAprotease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (16/15);  total (16/15)

CTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGTG  >  NC_000913/1850675‑1850940
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cTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcccctgcc                                                                                                                                 >  2:247104/1‑135 (MQ=255)
 tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                               <  1:1017196/139‑1 (MQ=255)
                  aGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGc                                                                                                              <  1:247104/139‑1 (MQ=255)
                       tGTACTGAGTGTCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTATGCTCttt                                                                                                         <  1:737483/139‑1 (MQ=255)
                                 ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  1:389003/139‑1 (MQ=255)
                                   ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                               >  2:423380/1‑105 (MQ=255)
                                   ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                               <  1:423380/105‑1 (MQ=255)
                                      aCAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTc                                                                                                                              >  2:1007203/1‑103 (MQ=255)
                                      aCAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTc                                                                                                                              <  1:1007203/103‑1 (MQ=255)
                                          aTCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGata                                                                                                    <  2:706002/125‑1 (MQ=255)
                                          aTCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGata                                                                                                    >  1:706002/1‑125 (MQ=255)
                                                 gCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                               >  1:212748/1‑91 (MQ=255)
                                                 gCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                               <  2:212748/91‑1 (MQ=255)
                                                             tttACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCaa                                                                   >  1:945139/1‑139 (MQ=255)
                                                                    cgacgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               >  1:465471/1‑104 (MQ=255)
                                                                    cgacgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  2:465471/104‑1 (MQ=255)
                                                                                  cccGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACAcgggcg                                              >  1:1075239/1‑139 (MQ=255)
                                                                                      cTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGata                                                                                                    <  2:1113588/81‑1 (MQ=255)
                                                                                      cTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGata                                                                                                    >  1:1113588/1‑81 (MQ=255)
                                                                                             gCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCggg                                   <  2:1075239/139‑1 (MQ=255)
                                                                                                   tatTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAg                             >  2:460121/1‑139 (MQ=255)
                                                                                                           tATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTaca                                                    >  1:317330/1‑108 (MQ=255)
                                                                                                           tATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTaca                                                    <  2:317330/108‑1 (MQ=255)
                                                                                                            aTCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  1:323343/64‑1 (MQ=255)
                                                                                                            aTCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               >  2:323343/1‑64 (MQ=255)
                                                                                                                 cATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  2:1031861/59‑1 (MQ=255)
                                                                                                                 cATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               >  1:1031861/1‑59 (MQ=255)
                                                                                                                       aTCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTtatat         >  1:534455/1‑139 (MQ=255)
                                                                                                                              gcgcTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  2:779781/46‑1 (MQ=255)
                                                                                                                              gcgcTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               >  1:779781/1‑46 (MQ=255)
                                                                                                                              gcgcTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGgtg  >  2:870306/1‑139 (MQ=255)
                                                                                                                              gcgcTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGgtg  >  1:883433/1‑139 (MQ=255)
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CTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGTG  >  NC_000913/1850675‑1850940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: