Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,875,056 (C)6→5 coding (521/822 nt) yeaM ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,875,0510C.100.0% 132.7 / NA 32coding (526/822 nt)yeaMputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (15/17);  total (15/17)

TTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAC  >  NC_000913/1874915‑1875168
                                                                                                                                        |                                                                                                                     
ttCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccc                                                                                                                     >  1:671677/1‑139 (MQ=255)
  cACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccc                                                                                                                   >  1:56742/1‑139 (MQ=255)
       aTCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCg                                                                                                             >  1:392546/1‑139 (MQ=255)
        tCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGa                                                                                                            >  1:147363/1‑139 (MQ=255)
          ccTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACa                                                                                                          >  2:120563/1‑139 (MQ=255)
                 cgcCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccca                                                                                                                  <  1:821551/125‑2 (MQ=255)
                 cgcCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccca                                                                                                                  >  2:821551/1‑124 (MQ=255)
                      tAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGcc                                                                                              <  1:120563/139‑1 (MQ=255)
                       aaTCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCca                                                                                             <  2:147363/139‑1 (MQ=255)
                         tCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                           <  2:841142/139‑1 (MQ=255)
                         tCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                           <  2:56742/139‑1 (MQ=255)
                              tGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCAtctc                                                                                      >  2:200374/1‑139 (MQ=255)
                                  aCTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTccacc                                                                                  >  2:966643/1‑139 (MQ=255)
                                      ctgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCg                                                                              >  1:232807/1‑139 (MQ=255)
                                       tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTTCTATCAGTCGGGCAAGATTGCCTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccac                                                                                                                 <  2:662499/104‑3 (MQ=255)
                                       tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGTAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCaatc                                                                                           <  2:730253/125‑1 (MQ=255)
                                       tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccac                                                                                                                 >  1:662499/1‑102 (MQ=255)
                                       tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                           >  1:730253/1‑125 (MQ=255)
                                        gACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTg                                                                            <  1:327962/139‑1 (MQ=255)
                                         aCGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGc                                                                           <  2:315325/139‑1 (MQ=255)
                                           gCCACTGGCGAAAACTCAACCCCTTCTCTTTTACTACCAGTCGGGCAACATTGCGTTCACTCACCGCAAAAACGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCgg                                                                         <  2:972206/139‑1 (MQ=255)
                                                            aaCCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGcc                                                                                              >  1:1087067/1‑101 (MQ=255)
                                                            aaCCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGcc                                                                                              <  2:1087067/101‑1 (MQ=255)
                                                                   tctcTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                           >  1:765995/1‑97 (MQ=255)
                                                                   tctcTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                           <  2:765995/97‑1 (MQ=255)
                                                                      ctTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAgg                                              <  1:966643/139‑1 (MQ=255)
                                                                      ctTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAgg                                              >  2:43072/1‑139 (MQ=255)
                                                                               tCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccca                                                                                                                  >  1:429139/1‑62 (MQ=255)
                                                                               tCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccca                                                                                                                  <  2:429139/63‑2 (MQ=255)
                                                                                 aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCca                                                                                             <  1:20388/81‑1 (MQ=255)
                                                                                 aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCca                                                                                             >  2:20388/1‑81 (MQ=255)
                                                                                      ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGcc                                                                                              >  1:710701/1‑75 (MQ=255)
                                                                                      ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGcc                                                                                              <  2:710701/75‑1 (MQ=255)
                                                                                                cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAgg                    >  1:970535/1‑139 (MQ=255)
                                                                                                 gTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAggg                   >  1:805544/1‑139 (MQ=255)
                                                                                                   tCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg                 <  1:672951/139‑1 (MQ=255)
                                                                                                     aCTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtg                                      <  2:77239/116‑1 (MQ=255)
                                                                                                     aCTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtg                                      >  1:77239/1‑116 (MQ=255)
                                                                                                              aaaaaaGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACa      <  1:200374/139‑1 (MQ=255)
                                                                                                                  aaGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAc  <  2:805544/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                     
TTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAC  >  NC_000913/1874915‑1875168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: