Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,214,339 (C)6→5 coding (306/1686 nt) yehU ← inner membrane putative sensory kinase in two‑component system with YehT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,214,3340C.100.0% 132.8 / NA 32coding (311/1686 nt)yehUinner membrane putative sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (19/13);  total (19/13)

GCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAA  >  NC_000913/2214196‑2214467
                                                                                                                                          |                                                                                                                                     
gCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGc                                                                                                                                       <  1:580729/139‑1 (MQ=255)
  gacgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                     <  1:1118202/139‑1 (MQ=255)
  gacgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                     <  1:362501/139‑1 (MQ=255)
      aaCGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCGGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccat                                                                                                                                 >  1:491985/1‑137 (MQ=255)
          tGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCGGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGaa                                                                                                                            <  2:491985/139‑1 (MQ=255)
                                 ttAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAg                                                                                                     <  1:188913/139‑1 (MQ=255)
                                   aaaGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGcc                                                                                                   >  2:932604/1‑139 (MQ=255)
                                   aaaGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGcc                                                                                                   >  1:543082/1‑139 (MQ=255)
                                          ttATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAg                                                                                            >  2:609646/1‑139 (MQ=255)
                                                 tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                     <  2:68782/92‑1 (MQ=255)
                                                 tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                     >  1:68782/1‑92 (MQ=255)
                                                 tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcg                                                                                     >  1:291908/1‑139 (MQ=255)
                                                        gcggcgGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccccca                                                                                                                                  <  2:478666/88‑2 (MQ=255)
                                                        gcggcgGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccccca                                                                                                                                  >  1:478666/1‑87 (MQ=255)
                                                              gATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                    >  2:911917/1‑111 (MQ=255)
                                                              gATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATAATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                    <  1:911917/111‑1 (MQ=255)
                                                                 cAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGa                                                                     <  1:609646/139‑1 (MQ=255)
                                                                           tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc                                                           >  1:162866/1‑139 (MQ=255)
                                                                                  ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACCTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc                                                    >  1:1019796/1‑139 (MQ=255)
                                                                                        cGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                     <  2:1058465/53‑1 (MQ=255)
                                                                                        cGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                     >  1:1058465/1‑53 (MQ=255)
                                                                                          aGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTAt                                            <  2:291908/139‑1 (MQ=255)
                                                                                              aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                        <  2:543082/139‑1 (MQ=255)
                                                                                                    cAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTa                                  >  1:421335/1‑139 (MQ=255)
                                                                                                      aCGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                    >  1:285634/1‑71 (MQ=255)
                                                                                                      aCGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                    <  2:285634/71‑1 (MQ=255)
                                                                                                      aCGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTAtt                                >  2:526510/1‑139 (MQ=255)
                                                                                                      aCGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTAtt                                <  2:162866/139‑1 (MQ=255)
                                                                                                         aTGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGc                             >  1:183783/1‑139 (MQ=255)
                                                                                                          tGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                     <  2:243174/35‑1 (MQ=255)
                                                                                                          tGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                     >  1:243174/1‑35 (MQ=255)
                                                                                                          tGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGTCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTAtt                                <  1:704848/135‑1 (MQ=255)
                                                                                                          tGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTAtt                                >  2:704848/1‑135 (MQ=255)
                                                                                                          tGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCa                            >  1:887964/1‑139 (MQ=255)
                                                                                                          tGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCa                            >  2:84418/1‑139 (MQ=255)
                                                                                                            gTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAAt                          >  1:291273/1‑139 (MQ=255)
                                                                                                             tCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATa                         >  1:26973/1‑139 (MQ=255)
                                                                                                                agaTCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGaa                      <  2:291273/139‑1 (MQ=255)
                                                                                                                      tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                               <  2:573063/60‑1 (MQ=255)
                                                                                                                      tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                               >  1:573063/1‑60 (MQ=255)
                                                                                                                           cTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg           <  2:26973/139‑1 (MQ=255)
                                                                                                                              aaCGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCaa        <  2:1125354/139‑1 (MQ=255)
                                                                                                                              aaCGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCaa        >  1:508323/1‑139 (MQ=255)
                                                                                                                                    gTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCaaa  >  1:203050/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                     
GCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAA  >  NC_000913/2214196‑2214467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: