Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,782,393	(C)5→4	coding (334/4581 nt)	ypjA ←	adhesin‑like autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,782,389	0	C	.	100.0%	267.0 / NA	63	coding (338/4581 nt)	ypjA	adhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (30/33);  total (30/33)

TACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAA  >  NC_000913/2782252‑2782521                                                                                                                                          |                                                                                                                                     tACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAcc                                                                                                                                     <  2:415439/139‑1 (MQ=255)         tCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATg                                                                                                                            >  1:890282/1‑139 (MQ=255)          cAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccccgtca                                                                                                                               <  2:470086/136‑5 (MQ=255)          cAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccccgtca                                                                                                                               >  1:470086/1‑132 (MQ=255)             tGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAAtt                                                                                                                        >  1:825117/1‑139 (MQ=255)               aGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGa                                                                                                                      <  2:847717/139‑1 (MQ=255)                 tATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACt                                                                                                                    <  2:825117/139‑1 (MQ=255)                    tCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTc                                                                                                                 >  1:184791/1‑139 (MQ=255)                      cATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAACCATACTGATTGCCACTCTCGATTGTCATACCTGTGGAAATA‑CCCCTTCATGAATTGactgactg                                                                                                               >  2:611810/1‑139 (MQ=255)                        ttCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGcc                                                                                                             >  2:689724/1‑139 (MQ=255)                        ttCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGcc                                                                                                             <  2:85403/139‑1 (MQ=255)                           gCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCAcc                                                                                                          >  1:147505/1‑139 (MQ=255)                                ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     <  2:890282/139‑1 (MQ=255)                                ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     <  1:363664/139‑1 (MQ=255)                                ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     <  1:689724/139‑1 (MQ=255)                                      ctctACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               <  2:184791/139‑1 (MQ=255)                                      ctctACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               <  2:486822/139‑1 (MQ=255)                                      ctctACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               <  1:245065/139‑1 (MQ=255)                                           cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                                          >  2:791810/1‑139 (MQ=255)                                               tGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAcc                                                                                                                                     <  1:837883/93‑1 (MQ=255)                                               tGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAcc                                                                                                                                     >  2:837883/1‑93 (MQ=255)                                               tGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAg                                                                                      >  2:337869/1‑139 (MQ=255)                                                 aaGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGcc                                                                                    >  1:860565/1‑139 (MQ=255)                                                 aaGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGcc                                                                                    >  2:72805/1‑139 (MQ=255)                                                 aaGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGcc                                                                                    >  2:643989/1‑139 (MQ=255)                                                        gCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCca                                                                             <  1:72805/139‑1 (MQ=255)                                                            ttAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACaaa                                                                         >  1:586804/1‑139 (MQ=255)                                                              aaTTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               >  2:360199/1‑115 (MQ=255)                                                              aaTTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               <  1:360199/115‑1 (MQ=255)                                                                   tCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               <  1:438849/110‑1 (MQ=255)                                                                   tCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               >  2:438849/1‑110 (MQ=255)                                                                   tCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                                  >  1:363186/1‑139 (MQ=255)                                                                   tCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                                  <  1:643989/139‑1 (MQ=255)                                                                    cGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTAt                                                                 <  1:840642/139‑1 (MQ=255)                                                                      ttCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGa                                                               <  2:465737/139‑1 (MQ=255)                                                                       tCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGAc                                                              <  1:337869/139‑1 (MQ=255)                                                                          aTTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTc                                                           <  2:363186/139‑1 (MQ=255)                                                                            tGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCt                                                         <  2:860565/139‑1 (MQ=255)                                                                            tGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCt                                                         <  1:791810/139‑1 (MQ=255)                                                                            tGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCt                                                         <  1:656599/139‑1 (MQ=255)                                                                              cTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGGCTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCtat                                                       <  1:611810/139‑1 (MQ=255)                                                                                        cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     <  1:630618/83‑1 (MQ=255)                                                                                        cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               <  1:462871/89‑1 (MQ=255)                                                                                        cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               >  2:462871/1‑89 (MQ=255)                                                                                        cTTTATAAACGTCCTGATAGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     >  2:630618/1‑83 (MQ=255)                                                                                                 cGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACt                                                                                                                    <  1:803982/59‑1 (MQ=255)                                                                                                 cGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCAGTCATGAATTGACt                                                                                                                    >  2:803982/1‑59 (MQ=255)                                                                                                   tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CTCCGTCATGAATTGACTGTCTGCCa                                                                                                            <  2:769143/65‑1 (MQ=255)                                                                                                   tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCca                                                                             >  1:936603/1‑96 (MQ=255)                                                                                                   tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCca                                                                             <  2:936603/96‑1 (MQ=255)                                                                                                   tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGAtt                                   >  2:5251/1‑138 (MQ=255)                                                                                                   tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGAtt                                   <  1:5251/138‑1 (MQ=255)                                                                                                   tCCTGATTGCCACTCTCGATAGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCa                                                                                                            >  1:769143/1‑65 (MQ=255)                                                                                                    ccTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                                  >  2:1003657/1‑106 (MQ=255)                                                                                                    ccTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                                  <  1:1003657/106‑1 (MQ=255)                                                                                                       gATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTa                                                                                                      <  2:89523/67‑1 (MQ=255)                                                                                                       gATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTa                                                                                                      >  1:89523/1‑67 (MQ=255)                                                                                                                  tcGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATccac                                         <  2:1131955/117‑1 (MQ=255)                                                                                                                  tcGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATccac                                         >  1:1131955/1‑117 (MQ=255)                                                                                                                       tGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATa              >  1:1101868/1‑139 (MQ=255)                                                                                                                                  tgGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGa   >  2:10034/1‑139 (MQ=255)                                                                                                                                   ggAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATg                                                                                                   >  1:668577/1‑42 (MQ=255)                                                                                                                                   ggAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGa                                     >  1:1021580/1‑104 (MQ=255)                                                                                                                                   ggAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGa                                     <  2:1021580/104‑1 (MQ=255)                                                                                                                                   ggAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGaa  >  1:402566/1‑139 (MQ=255)                                                                                                                                   ggAAATA‑CCCCGTCATGAATTCACTGTCTGCCACCGTTAATg                                                                                                   <  2:668577/42‑1 (MQ=255)                                                                                                                                    gAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtat                                                                                            >  1:372657/1‑48 (MQ=255)                                                                                                                                    gAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtat                                                                                            <  2:372657/48‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     TACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAA  >  NC_000913/2782252‑2782521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑