Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,330 (G)8→7 noncoding (72/87 nt) leuQ ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,3230G.100.0% 176.1 / NA 44noncoding (79/87 nt)leuQtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (21/23);  total (21/23)

TGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCG  >  NC_000913/4606185‑4606441
                                                                                                                                          |                                                                                                                      
tGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAg                                                                                                                        <  2:965150/139‑1 (MQ=255)
   tatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggg                                                                                                                     <  1:17193/139‑1 (MQ=255)
           cGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTg                                                                                                            <  1:577983/139‑1 (MQ=255)
                   gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCa                                                                                                    <  2:982286/139‑1 (MQ=255)
                      aGGGGGGGGACTTGAACCCCCACGTCCGTAAGTACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAAcccc                                                                                                      >  2:283580/1‑134 (MQ=255)
                      aGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCGCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAAcccc                                                                                                      <  1:283580/134‑1 (MQ=255)
                                cTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACg                                                                                                  >  2:581688/1‑128 (MQ=255)
                                cTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACg                                                                                                  <  1:581688/128‑1 (MQ=255)
                                   gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                    <  1:261757/139‑1 (MQ=255)
                                           aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCa                                                                                                    <  1:1109039/115‑1 (MQ=255)
                                           aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCa                                                                                                    >  2:1109039/1‑115 (MQ=255)
                                                 gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                                         <  1:1078362/120‑1 (MQ=255)
                                                 gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                                         >  2:1078362/1‑120 (MQ=255)
                                                       acacTAGCACCTGAAGCTAGCGCGTCTACCCATTCCGCCACCATCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt                                                                <  2:316764/139‑1 (MQ=37)
                                                         acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGt                                                                                             <  1:588852/108‑1 (MQ=255)
                                                         acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGt                                                                                             >  2:588852/1‑108 (MQ=255)
                                                         acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                                              >  1:1086004/1‑139 (MQ=255)
                                                         acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                                              >  2:1007440/1‑139 (MQ=255)
                                                                ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                                                       >  1:791729/1‑139 (MQ=255)
                                                                      gCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                 <  2:791729/139‑1 (MQ=255)
                                                                           cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                            >  2:475017/1‑139 (MQ=255)
                                                                                             cACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTa                          >  2:451780/1‑139 (MQ=255)
                                                                                                     cccAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt                  <  2:1086004/139‑1 (MQ=255)
                                                                                                         gTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCt              <  1:475017/139‑1 (MQ=255)
                                                                                                          tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                          >  1:928980/1‑62 (MQ=255)
                                                                                                          tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                          <  2:928980/62‑1 (MQ=255)
                                                                                                              aGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCAc         >  1:94876/1‑139 (MQ=255)
                                                                                                              aGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCAc         >  1:814973/1‑139 (MQ=255)
                                                                                                               gCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg        <  1:451780/139‑1 (MQ=255)
                                                                                                                  atatCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg     <  1:1007440/139‑1 (MQ=255)
                                                                                                                  atatCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg     <  2:94876/139‑1 (MQ=255)
                                                                                                                    atCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCa                                                                                                    >  1:630315/1‑42 (MQ=21)
                                                                                                                    atCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCa                                                                                                    <  2:630315/42‑1 (MQ=21)
                                                                                                                    atCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                          >  2:193760/1‑52 (MQ=18)
                                                                                                                    atCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                          <  1:193760/52‑1 (MQ=18)
                                                                                                                    atCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                                                       <  1:739001/71‑1 (MQ=255)
                                                                                                                    atCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                                                       >  2:739001/1‑71 (MQ=255)
                                                                                                                    atCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                   <  2:112539/91‑1 (MQ=255)
                                                                                                                    atCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                   >  1:112539/1‑91 (MQ=255)
                                                                                                                    atCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgc   >  1:588958/1‑139 (MQ=255)
                                                                                                                         cGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                           >  1:453960/1‑94 (MQ=255)
                                                                                                                         cGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                           <  2:453960/94‑1 (MQ=255)
                                                                                                                           tGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg  <  1:131716/133‑1 (MQ=255)
                                                                                                                           tGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg  >  2:131716/1‑133 (MQ=255)
                                                                                                                               ttttGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                     <  2:889224/62‑1 (MQ=255)
                                                                                                                               ttttGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                     >  1:889224/1‑62 (MQ=255)
                                                                                                                                          |                                                                                                                      
TGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCG  >  NC_000913/4606185‑4606441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: