| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 380,022 | (G)10→9 | intergenic (‑141/+47) | frmR ← / ← yaiO | regulator protein that represses frmRAB operon/outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 380,013 | 0 | G | . | 98.4% | 256.0 / ‑2.6 | 62 | intergenic (‑132/+56) | frmR/yaiO | regulator protein that represses frmRAB operon/outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base . (34/27); total (35/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
TAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACC > NC_000913/379914‑380136 | taGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggcta < 1:590919/111‑4 (MQ=255)taGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggcta > 2:590919/1‑108 (MQ=255)taGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgc > 2:825630/1‑139 (MQ=255) gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcg > 1:581533/1‑139 (MQ=255) gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcg > 2:291078/1‑139 (MQ=255) tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa < 2:393577/134‑1 (MQ=255) tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa > 1:393577/1‑134 (MQ=255) gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa > 1:129129/1‑139 (MQ=255) gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa > 1:92757/1‑139 (MQ=255) tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAt > 1:400951/1‑139 (MQ=255) tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAgg < 2:1110174/102‑1 (MQ=255) tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAgg > 1:1110174/1‑102 (MQ=255) tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAtt > 1:1930/1‑139 (MQ=255) cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAAt < 2:555694/83‑1 (MQ=255) cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAAt > 1:555694/1‑83 (MQ=255) cTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa > 2:973673/1‑139 (MQ=255) ataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACg < 2:581533/139‑1 (MQ=255) tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAgg > 1:984677/1‑85 (MQ=255) tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAgg < 2:984677/85‑1 (MQ=255) tatataACACTGGAGAATAAAATTTATCCGGCGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt > 2:716496/1‑139 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc > 2:673177/1‑139 (MQ=255) ataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa < 1:291078/139‑1 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgcc > 1:662032/1‑80 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgcc < 2:662032/80‑1 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg < 1:783552/102‑1 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg > 2:783552/1‑102 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa > 2:293552/1‑107 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa < 1:293552/107‑1 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGt < 2:852315/119‑1 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGt > 1:852315/1‑119 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa > 1:858614/1‑139 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa > 1:1008065/1‑139 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa > 1:759666/1‑139 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa > 2:727834/1‑139 (MQ=255) ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg < 2:1930/139‑1 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa < 1:556511/137‑1 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa > 2:556511/1‑137 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCAGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt > 1:387513/1‑127 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCAGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt < 2:387513/127‑1 (MQ=255) aTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGt < 2:1008065/139‑1 (MQ=255) tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg < 2:225388/46‑1 (MQ=255) tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg > 1:225388/1‑46 (MQ=255) tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCgg < 2:122332/59‑1 (MQ=255) tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCgg > 1:122332/1‑59 (MQ=255) tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGc > 2:1065667/1‑60 (MQ=255) tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGc < 1:1065667/60‑1 (MQ=255) aTCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcat < 2:129129/139‑1 (MQ=255) gTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGAc > 1:414313/1‑104 (MQ=255) gTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGAc < 2:414313/104‑1 (MQ=255) tGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTg < 2:858614/139‑1 (MQ=255) aaTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa > 1:431013/1‑64 (MQ=255) aaTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa < 2:431013/64‑1 (MQ=255) aTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTgggg < 2:759666/139‑1 (MQ=255) tgtgGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGa < 1:753161/78‑1 (MQ=255) tgtgGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGa > 2:753161/1‑78 (MQ=255) gtgGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtg < 1:673177/139‑1 (MQ=255) tCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcat < 1:53461/124‑1 (MQ=255) tCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcat > 2:53461/1‑124 (MQ=255) tCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGt > 1:135101/1‑139 (MQ=255) cGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAgg > 1:798994/1‑44 (MQ=255) cGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAgg < 2:798994/44‑1 (MQ=255) gAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa > 2:323591/1‑66 (MQ=255) gAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa < 1:323591/66‑1 (MQ=255) gAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAACCATCGGTTGGGGTGCCGTAcc > 2:980294/1‑139 (MQ=255) aaaCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTg > 2:1131574/1‑125 (MQ=255) aaaCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTg < 1:1131574/125‑1 (MQ=255) | TAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACC > NC_000913/379914‑380136 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |