Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,759,680	(C)5→6	coding (844/1221 nt)	sufS ←	cysteine desulfurase, stimulated by SufE, selenocysteine lyase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,759,675	1	.	C	100.0%	141.1 / NA	40	G283G (GGC→GGG)	sufS	cysteine desulfurase, stimulated by SufE, selenocysteine lyase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (23/17);  total (23/17)

GGGCCATAGAGAGTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATG‑CCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGC  >  NC_000913/1759541‑1759803                                                                                                                                        |                                                                                                                                 gggCCATAGAGAGTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATG‑cccc                                                                                                                              <  1:729195/139‑1 (MQ=255)   gCCATAGAGAGTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATG‑cccccc                                                                                                                            <  1:1059242/139‑2 (MQ=255)         gagagTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTAtt                                                                                                                       <  1:251797/139‑1 (MQ=255)              tGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGgtg                                                                                                                  >  1:410314/1‑139 (MQ=255)                      ggTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTGATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATG‑ccccccggta                                                                                                                        <  2:718521/124‑6 (MQ=255)                      ggTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTGCAGCGCCGCGCCAAGACCAATGATG‑ccccccggta                                                                                                                        >  1:718521/1‑119 (MQ=255)                                   gCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCAta                                                                                             <  2:707437/139‑2 (MQ=255)                                      gtgATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTg                                                                                          >  2:279184/1‑139 (MQ=255)                                        gATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                                        <  2:955466/139‑1 (MQ=255)                                                 tAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                                        >  2:580238/1‑130 (MQ=255)                                                 tAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                                        <  1:580238/130‑1 (MQ=255)                                                 tAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAg                                                                               >  2:102288/1‑139 (MQ=255)                                                 tAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAg                                                                               >  1:591026/1‑139 (MQ=255)                                                 tAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAg                                                                               >  1:800624/1‑139 (MQ=255)                                                       aTCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTg                                                                         >  2:314956/1‑139 (MQ=255)                                                        tCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATG‑ccccccggta                                                                                                                        >  2:795167/1‑85 (MQ=255)                                                        tCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATG‑ccccccggta                                                                                                                        <  1:795167/90‑6 (MQ=255)                                                         cAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGcc                                                                       <  2:591026/139‑1 (MQ=255)                                                         cAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGcc                                                                       <  2:410314/139‑1 (MQ=255)                                                         cAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGcc                                                                       <  1:102288/139‑1 (MQ=255)                                                              tCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATG‑ccccccggta                                                                                                                        >  1:221521/1‑79 (MQ=255)                                                              tCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATG‑ccccccggta                                                                                                                        <  2:221521/84‑6 (MQ=255)                                                                tGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATG‑cccc                                                                                                                              >  2:1006201/1‑76 (MQ=255)                                                                tGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATG‑cccc                                                                                                                              <  1:1006201/76‑1 (MQ=255)                                                                      tACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTg                                                          >  2:672868/1‑139 (MQ=255)                                                                         tCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACg                                                       <  2:800624/139‑1 (MQ=255)                                                                          cGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTc                                                                                  <  1:545527/111‑1 (MQ=255)                                                                          cGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTc                                                                                  >  2:545527/1‑111 (MQ=255)                                                                            gCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCtt                                                                                       >  1:1003260/1‑104 (MQ=255)                                                                            gCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCtt                                                                                       <  2:1003260/104‑1 (MQ=255)                                                                                       aaGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                                        >  1:1038218/1‑92 (MQ=255)                                                                                       aaGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                                        <  2:1038218/92‑1 (MQ=255)                                                                                          ccccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAAcc                                      >  1:694878/1‑139 (MQ=255)                                                                                          ccccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAAcc                                      >  2:842245/1‑139 (MQ=255)                                                                                          ccccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAAcc                                      >  1:467204/1‑139 (MQ=255)                                                                                          ccccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAAcc                                      >  2:113558/1‑139 (MQ=255)                                                                                             cAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                                        >  1:1093680/1‑86 (MQ=255)                                                                                             cAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                                        <  2:1093680/86‑1 (MQ=255)                                                                                             cAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATG‑ccc                                                                                                                               >  1:477224/1‑46 (MQ=255)                                                                                             cAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATG‑ccc                                                                                                                               <  2:477224/46‑1 (MQ=255)                                                                                              aGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTc                                                                                  <  2:299628/91‑1 (MQ=255)                                                                                              aGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTc                                                                                  >  1:299628/1‑91 (MQ=255)                                                                                                cgcCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGccccc                                >  1:561604/1‑139 (MQ=255)                                                                                                              ccAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCa                  <  1:113558/139‑1 (MQ=255)                                                                                                               cAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCAt                 >  1:608945/1‑139 (MQ=255)                                                                                                                 gcgccgcgccAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCAtct               >  1:412076/1‑139 (MQ=255)                                                                                                                    ccgcgccAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                                        <  1:470358/63‑1 (MQ=255)                                                                                                                    ccgcgccAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                                        <  1:20786/63‑1 (MQ=255)                                                                                                                    ccgcgccAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                                        >  2:470358/1‑63 (MQ=255)                                                                                                                    ccgcgccAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                                        >  2:20786/1‑63 (MQ=255)                                                                                                                                 aaTGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGcaaca      <  2:7552/132‑1 (MQ=255)                                                                                                                                 aaTGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGcaaca      >  1:7552/1‑132 (MQ=255)                                                                                                                                  atgatgcCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGc  >  1:941390/1‑135 (MQ=255)                                                                                                                                  atgatgcCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGc  <  2:941390/135‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 GGGCCATAGAGAGTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATG‑CCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGC  >  NC_000913/1759541‑1759803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑