| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,330,357 | +T | coding (1395/1434 nt) | dacB → | D‑alanyl‑D‑alanine carboxypeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,330,353 | 1 | . | T | 97.2% | 112.0 / ‑3.9 | 36 | coding (1391/1434 nt) | dacB | D‑alanyl‑D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (18/17); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑T‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTT > NC_000913/3330223‑3330456 | ttGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑t‑ttttgaa < 1:888057/139‑5 (MQ=255) tGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑t‑ttttgaaa < 2:866567/139‑6 (MQ=255) ggggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGt < 1:1026209/139‑1 (MQ=255) ggggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGt < 1:604646/139‑1 (MQ=255) aCCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAg > 1:466326/1‑139 (MQ=255) cTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGt > 1:175139/1‑127 (MQ=255) cTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGt < 2:175139/127‑1 (MQ=255) ggCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGatat > 2:404669/1‑139 (MQ=255) cGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGt < 1:417098/123‑1 (MQ=255) cGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGt > 2:417098/1‑123 (MQ=255) cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGata < 1:563230/118‑1 (MQ=255) cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGata > 2:563230/1‑118 (MQ=255) gggCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGCTATTTa > 1:679700/1‑117 (MQ=255) gggCAACGAATGGCGTTTGAGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTa < 2:679700/117‑1 (MQ=255) cAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAc > 2:540495/1‑139 (MQ=255) aCGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAccc < 1:463457/139‑1 (MQ=255) aaTGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCgg < 1:404669/139‑1 (MQ=255) gCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGt < 1:597784/88‑1 (MQ=255) gCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGt > 2:597784/1‑88 (MQ=255) tgttCAATAGCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGACGTATTACGTTAGTGCT‑TGTTTGAAAGCCGTCTGTATCAAGATATTTATCAGAACAATTAACCAGAAAGTTTCCCGGGAACATGGcc < 2:130455/139‑1 (MQ=255) tGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGata > 1:1116099/1‑75 (MQ=255) tGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGata < 2:1116099/75‑1 (MQ=255) tGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACaa > 1:754345/1‑139 (MQ=255) aGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGt < 1:789918/56‑1 (MQ=255) aGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGt > 2:789918/1‑56 (MQ=255) aaCCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCGCATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt > 1:713532/1‑139 (MQ=255) aCCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCtt < 1:540495/139‑1 (MQ=255) gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGtt > 2:799621/1‑139 (MQ=255) cGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGt > 1:647486/1‑48 (MQ=38) cGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGt > 1:575158/1‑48 (MQ=38) cGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGt < 2:575158/48‑1 (MQ=38) cGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGt < 2:647486/48‑1 (MQ=38) tAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAcccc > 2:353477/1‑83 (MQ=255) tAATCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAcccc < 1:353477/83‑1 (MQ=255) tCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGta > 2:144695/1‑40 (MQ=38) tCGCCGTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGta < 1:144695/40‑1 (MQ=255) gTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAg < 2:891459/51‑1 (MQ=255) gTATTCCGTTAGTGCGTT‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAg > 1:891459/1‑51 (MQ=255) | TTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑T‑TTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTT > NC_000913/3330223‑3330456 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |