Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,728,118	(C)8→9	coding (1254/1455 nt)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,728,110	1	.	C	100.0%	81.7 / NA	24	D421G (GAT→GGT)	xylB	xylulokinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (13/11);  total (13/11)

AGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCG‑CCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAC  >  NC_000913/3727980‑3728234                                                                                                                                    |                                                                                                                               aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccccccc                                                                                                                       <  1:203737/139‑1 (MQ=255)      cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑ccccccccccgta                                                                                                                  <  2:281078/139‑6 (MQ=255)                cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAg                                                                                                         <  2:1126685/139‑1 (MQ=255)                     cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                    <  1:778418/139‑1 (MQ=255)                     cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑c                                                                                                                              >  1:1122622/1‑112 (MQ=255)                     cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑c                                                                                                                              <  2:1122622/112‑1 (MQ=255)                            aTGCTACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGGAATCTAGCTGCTGACCGc                                                                                              <  1:866852/139‑1 (MQ=255)                                  cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATc                                                                                       >  1:346900/1‑139 (MQ=255)                                  atGTTCTAACGGTAGTTGCGGCAAAAATTCAATGAGCGATTTCTCTGGGTTCGCCGCGTTCCGCGTCCGCCTTGCTGCGCCCGGAGGTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGatat                                                                                        <  2:955278/138‑1 (MQ=255)                                          aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTCCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGc                                                                                >  1:78278/1‑139 (MQ=255)                                             gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑ccccc                                                                                                                          >  1:198710/1‑92 (MQ=255)                                             gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑ccccc                                                                                                                          <  2:198710/92‑1 (MQ=255)                                               aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCCAGCGGCTGACCGCTGATATCCGCCAGCATCTg                                                                           >  1:497255/1‑139 (MQ=255)                                               aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                          >  2:260508/1‑139 (MQ=255)                                                   gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                       <  2:78278/139‑1 (MQ=255)                                                      gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGt                                                                   >  1:949089/1‑139 (MQ=255)                                                                    aGCGATTTCTCTGGATTCGCCGCGAACTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGTTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcg‑cc                                                    >  2:70506/1‑139 (MQ=255)                                                                     gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                         >  2:647420/1‑102 (MQ=255)                                                                     gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                         <  1:647420/102‑1 (MQ=255)                                                                             tctGGATTTGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGACCCACATC‑CCCCCCCCGTACGGTAATCGCGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCG‑CCCCGCCCCCa                                           <  1:675601/139‑1 (MQ=255)                                                                               tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCG‑CCCCGCCCCCAAt                                         <  1:241873/139‑1 (MQ=255)                                                                               tGGATTCGCCGCGAACTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGTTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCG‑CCCCGCCCCCAAt                                         <  1:70506/139‑1 (MQ=255)                                                                                      gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCG‑CCCCGCCCCCAATCAACGTa                                  >  1:634857/1‑139 (MQ=255)                                                                                       ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCATCCCCCCCCCCGCCCGGCAATCGCGCCGCTCACCGCTGATTTTCCCCACCACCTCTCTCCCGTCCCCCCTACCCGCCCCCGCCCCCatcccccca                                   >  2:753238/1‑131 (MQ=255)                                                                                       ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCCTACGGTAACCGCACTGCCGGCCCCTGCGATCCGCCAGCATCCTGCGCCAGTACTCACCCCCCG‑cccccccccccaccaccgca                                  >  2:462050/1‑129 (MQ=255)                                                                                                  gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                     <  2:130253/93‑1 (MQ=255)                                                                                                  gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                     >  1:130253/1‑93 (MQ=255)                                                                                                          tgctgcGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCG‑CCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc               <  1:462050/139‑1 (MQ=255)                                                                                                           gctggGCCCAGTGCTGGCCCCACAT‑‑cccccccccc                                                                                                                     <  1:112574/35‑3 (MQ=255)                                                                                                           gctgcGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCG‑CCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg             >  2:929325/1‑139 (MQ=255)                                                                                                           gctgcGCCCAGTGCTGGCCCCACAT‑‑cccccccccc                                                                                                                     >  2:112574/1‑33 (MQ=255)                                                                                                              gcgcCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCG‑CCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAg          <  2:949089/139‑1 (MQ=255)                                                                                                                      tGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCG‑CCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc  >  1:157352/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                               AGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCG‑CCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAC  >  NC_000913/3727980‑3728234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑