| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,609,336 | (C)7→8 | intergenic (+13/‑78) | yjjG → / → prfC | dUMP phosphatase/peptide chain release factor RF‑3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,609,329 | 1 | . | C | 92.9% | 41.3 / ‑2.8 | 14 | intergenic (+6/‑85) | yjjG/prfC | dUMP phosphatase/peptide chain release factor RF‑3 |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (9/4); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01 | |||||||||||
TCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTG > NC_000913/4609242‑4609443 | tCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CCTCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcg > 1:95667/1‑139 (MQ=255) tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCCC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTCCTc > 1:801738/1‑139 (MQ=255) cgcgCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CCTCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCa > 1:17023/1‑139 (MQ=255) cgcgCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CCT‑c > 1:959282/1‑68 (MQ=255) cgcgCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CCT‑c < 2:959282/68‑1 (MQ=255) cACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CCTCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga < 2:17023/139‑1 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CCTCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAATAATTATGa > 2:689379/1‑139 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CCTCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGa > 1:1024654/1‑139 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CCTCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGa > 1:479214/1‑139 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CCTCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGa > 1:64707/1‑139 (MQ=255) tcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CCTCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg < 2:64707/139‑1 (MQ=255) tGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CCTCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCg > 1:920488/1‑105 (MQ=255) tGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CCTCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCg < 2:920488/105‑1 (MQ=255) cTCCTGTGTAAACACTGATTG‑CCTCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATg < 1:243198/85‑1 (MQ=255) cTCCTGTGTAAACACTGATTG‑CCTCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATg > 2:243198/1‑85 (MQ=255) cTCCTGTGTAAACACTGATTG‑CCTCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTg > 1:550608/1‑139 (MQ=255) | TCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CCT‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTG > NC_000913/4609242‑4609443 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |