Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	3,240,984	(G)7→10	coding (1041/1245 nt)	sstT →	sodium:serine/threonine symporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3240977	0 (0.000)	52 (1.140) +GGG	34/190	0.2	100%	coding (1034/1245 nt)	sstT	sodium:serine/threonine symporter
	?	NC_000913	3240978 =	0 (0.000)					coding (1035/1245 nt)	sstT	sodium:serine/threonine symporter

GCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3240844‑3240977 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAA  >  NC_000913/3240978‑3241106                                                                                                                                       ||||                                                                                                                                  GCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTTCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGG                                                                                                                                 >  1:258310/1‑139 GCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGGGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGG                                                                                                                                 >  1:1053507/1‑139   GGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTACTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGG                                                                                                                               >  2:174873/1‑139         AGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGtctc                                                                                                                         <  1:637418/139‑5            AATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTG                                                                                                                      <  1:774448/139‑1            AATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTG                                                                                                                      <  2:258310/139‑1                 CTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCT                                                                                                                 <  1:1124219/139‑1                   ATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGA                                                                                                               >  2:944322/1‑139                       CCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCC                                                                                                           >  2:329630/1‑139                        CGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCA                                                                                                          <  1:542718/139‑1                        CGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCA                                                                                                          <  1:944322/139‑1                         GTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑GGGGGGGGGTCTCTGCTGCTGATCCCACT                                                                                                        <  1:1035397/139‑1                           GTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCACTG                                                                                                       <  1:329630/139‑1                            TTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCACTGG                                                                                                      >  2:1050887/1‑139                            TTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCACTGG                                                                                                      >  2:613842/1‑139                             TGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCACTGGC                                                                                                     <  1:613842/139‑1                                 GCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGGGTCTCTGCTGCTGATCCCCCTGGCCTG                                                                                                  >  1:211719/1‑139                                 GCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGT                                                                                                 >  1:628488/1‑139                                     GCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTATCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATA                                                                                             >  2:359548/1‑139                                      CTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTACTGTTGGGCGTGGTGGCTTCTCTGTGTGCCTGGGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATAT                                                                                            <  1:174873/139‑1                                       TGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATG                                                                                           <  1:1058538/139‑1                                          GGTTAATACGCTGGGTATTCCGGTCCATCTGCCCACGGGGCTGGTTTTGAGGGTGGGGGCTTCTTTGTGGGCCTGTGGCGCATCCGGGGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTC                                                                                        <  2:241551/139‑1                                                      GGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGCTCCTGCTGTTGATCCCATGGGCCTGTAATTTTTCGGGTATTTGGAAt                                                                            >  1:241551/1‑138                                                            TCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGtctc                                                                                                                         <  1:638100/88‑5                                                            TCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGtctc                                                                                                                         >  2:638100/1‑84                                                            TCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑GGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCG                                                                     <  1:392939/139‑1                                                              CGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCACTGGC                                                                                                     >  1:556231/1‑106                                                              CGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCACTGGC                                                                                                     <  2:556231/106‑1                                                                 TCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTC                                                                                                                        >  1:872972/1‑84                                                                 TCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTC                                                                                                                        <  2:872972/84‑1                                                                 TCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑GGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATG                                                                >  2:921295/1‑139                                                                              GGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑GGGGGGGGGTCTCTGCTGCTGATCCCA                                                                                                          <  2:354029/84‑1                                                                              GGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑GGGGGGGGGTCTCTGCTGCTGATCCCA                                                                                                          >  1:354029/1‑84                                                                                           AGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑GGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCAT                                      <  2:1053507/139‑1                                                                                             CGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCAT                                      >  2:702055/1‑139                                                                                               TGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCT                                                                                                                 <  2:97625/61‑1                                                                                               TGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCT                                                                                                                 >  1:97625/1‑61                                                                                               TGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCT                                                                                                                         >  1:554337/1‑53                                                                                               TGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCT                                                                                                                         <  2:554337/53‑1                                                                                                              GTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCACT                                                                                                        >  1:373715/1‑55                                                                                                              GTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCACT                                                                                                        <  2:373715/55‑1                                                                                                                  CTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCT                                                                                                   >  2:310596/1‑56                                                                                                                  CTGTGGCGCATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCT                                                                                                   <  1:310596/56‑1                                                                                                                   TGTGGCGCATCCGGCGTGGCGGGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGC                >  2:361188/1‑139                                                                                                                   TGTGGCGCATCCGGCGTGGC‑‑GGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGA              >  2:531445/1‑139                                                                                                                     TGGCGCATCCGGCGTGGCGGGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGA              >  1:928033/1‑139                                                                                                                     TGGCGCATCCGGCGTGGC‑‑GGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGA                                                                              <  1:763412/73‑1                                                                                                                     TGGCGCATCCGGCGTGGC‑‑GGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGA                                                                              >  2:763412/1‑73                                                                                                                       GCGCATCCGGCGTGGC‑‑GGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACC          >  2:393376/1‑139                                                                                                                           ATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCA                                                                                                          >  2:11806/1‑40                                                                                                                           ATCCGGCGTGGC‑GGGGGGGGGGTCTCTGCTGCTGATCCCA                                                                                                          <  1:11806/40‑1                                                                                                                               GGCGTGGCGGGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTG    <  1:702055/139‑1                                                                                                                                 CGTGGCGGGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAA  <  1:361188/139‑1                                                                                                                                        GGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGT                                                        >  2:1011782/1‑78                                                                                                                                        GGGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGT                                                        <  1:1011782/78‑1                                                                                                                                       ||||                                                                                                                                  GCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3240844‑3240977 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAA  >  NC_000913/3240978‑3241106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.