Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	475,667	T→C	S97P (TCA→CCA)	ybaY →	outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	475,667	0	T	C	100.0%	126.7 / NA	36	S97P (TCA→CCA)	ybaY	outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/17);  total (19/17)

CCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAACC  >  NC_000913/475536‑475799                                                                                                                                    |                                                                                                                                     ccGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCAtt                                                                                                                               <  2:807559/139‑1 (MQ=255)    tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCAtt                                                                                                                               <  2:622572/136‑1 (MQ=255)    tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCAtt                                                                                                                               >  1:622572/1‑136 (MQ=255)    tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAg                                                                                                                            >  1:170421/1‑139 (MQ=255)    tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAg                                                                                                                            >  1:742828/1‑139 (MQ=255)      tGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGGCGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCt                                                                                                                          >  2:352359/1‑139 (MQ=255)              tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCt                                                                                                                          <  1:114598/131‑1 (MQ=255)              tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCt                                                                                                                          >  2:114598/1‑131 (MQ=255)                      tCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTaa                                                                                                          >  1:974095/1‑139 (MQ=255)                         cACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAAccc                                                                                                       <  1:236328/139‑1 (MQ=255)                                             ctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcg                                                                                   >  2:771181/1‑139 (MQ=255)                                                aCCGTGACACTTTCTGTCGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                                <  1:771181/139‑1 (MQ=255)                                                aCCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                                <  2:170421/139‑1 (MQ=255)                                                aCCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                                <  1:790711/139‑1 (MQ=255)                                                     gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGc                                                                                                    <  1:270284/114‑1 (MQ=255)                                                     gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGc                                                                                                    >  2:270284/1‑114 (MQ=255)                                                     gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTAtt                                                                           >  2:1128432/1‑139 (MQ=255)                                                               gACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTg                                                                 >  1:405854/1‑139 (MQ=255)                                                                     tcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGa                                                           >  1:344076/1‑139 (MQ=255)                                                                        ttAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTGAGCTTTGGTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTa                                                        >  2:194486/1‑139 (MQ=255)                                                                                tGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCCTTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAAt                                                >  2:244715/1‑139 (MQ=255)                                                                                tGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTg                                                                                                                       <  2:1097327/68‑1 (MQ=255)                                                                                tGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTg                                                                                                                       >  1:1097327/1‑68 (MQ=255)                                                                                  cACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGa                                              <  2:476252/139‑1 (MQ=255)                                                                                    ccGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTACCCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTTAATGACa                                            <  2:742828/139‑1 (MQ=255)                                                                                      gTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACaaa                                          <  2:216521/139‑1 (MQ=255)                                                                                       tCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAAc                                         <  1:1128432/139‑1 (MQ=255)                                                                                                                       tGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTg         <  1:244715/139‑1 (MQ=255)                                                                                                                           ggTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCa     >  2:45647/1‑139 (MQ=255)                                                                                                                            gTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACaa                                           <  1:592503/100‑1 (MQ=255)                                                                                                                            gTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACaa                                           >  2:592503/1‑100 (MQ=255)                                                                                                                            gTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATa                       >  2:203305/1‑120 (MQ=255)                                                                                                                            gTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATa                       <  1:203305/120‑1 (MQ=255)                                                                                                                              aaaCAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAAcc  >  1:1047221/1‑139 (MQ=255)                                                                                                                               aaCAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgc                                                                                  <  2:183175/58‑1 (MQ=255)                                                                                                                               aaCAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgc                                                                                  >  1:183175/1‑58 (MQ=255)                                                                                                                                    |                                                                                                                                     CCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAACC  >  NC_000913/475536‑475799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑