Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	486,641	T→C	S36P (TCA→CCA)	mscK →	mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	486,641	0	T	C	100.0%	153.7 / NA	43	S36P (TCA→CCA)	mscK	mechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/23);  total (20/23)

TTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACC  >  NC_000913/486507‑486766                                                                                                                                       |                                                                                                                              ttcttcAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATc                                                                                                                           <  2:787388/139‑1 (MQ=255)       aGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATgg                                                                                                                     >  2:843959/1‑139 (MQ=255)                    ttcattcaTCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATc                                                                                                                           <  1:1051379/120‑1 (MQ=255)                    ttcattcaTCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATc                                                                                                                           >  2:1051379/1‑120 (MQ=255)                                 aCTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGc                                                                                           <  1:988553/139‑1 (MQ=255)                                      gTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGgcg                                                                                      <  1:843959/139‑1 (MQ=255)                                      gTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGgcg                                                                                      <  1:232543/139‑1 (MQ=255)                                       ttCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCATAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGgcgc                                                                                     >  1:935770/1‑139 (MQ=255)                                       ttCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCAt                                                                                                                            <  1:1118585/100‑1 (MQ=255)                                       ttCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCAt                                                                                                                            >  2:1118585/1‑100 (MQ=255)                                            gTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACtt                                                                                >  1:118483/1‑139 (MQ=255)                                            gTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACtt                                                                                >  1:1025026/1‑139 (MQ=255)                                             tATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTg                                                                               <  1:207000/139‑1 (MQ=255)                                             tATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTg                                                                               >  2:763536/1‑139 (MQ=255)                                                       gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaa                                                                     >  1:167400/1‑139 (MQ=255)                                                       gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaa                                                                     >  2:572333/1‑139 (MQ=255)                                                       gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaa                                                                     >  1:126717/1‑139 (MQ=255)                                                       gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaa                                                                     >  1:166477/1‑139 (MQ=255)                                                         tCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaata                                                                   <  2:126717/139‑1 (MQ=255)                                                           aCGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaataaa                                                                 >  1:13657/1‑139 (MQ=255)                                                                 ttttGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGa                                                                                                          <  2:968320/92‑1 (MQ=255)                                                                 ttttGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGa                                                                                                          >  1:968320/1‑92 (MQ=255)                                                                 ttttGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACaaaaa                                                           >  2:275438/1‑139 (MQ=255)                                                                        ttttCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCttt                                                    <  2:167400/139‑1 (MQ=255)                                                                        ttttCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCttt                                                    <  2:13657/139‑1 (MQ=255)                                                                             aGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                               <  1:699971/139‑1 (MQ=255)                                                                             aGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                               <  2:935770/139‑1 (MQ=255)                                                                                    aTTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACa                                        >  1:553650/1‑139 (MQ=255)                                                                                         ttttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTg                                   >  2:744891/1‑139 (MQ=255)                                                                                               ttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgcag                             <  1:744891/139‑1 (MQ=255)                                                                                               ttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgcag                             <  2:1025026/139‑1 (MQ=255)                                                                                               ttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgcag                             <  1:572333/139‑1 (MQ=255)                                                                                                tttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgcagc                            <  2:553650/139‑1 (MQ=255)                                                                                                         ttATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                               <  2:266016/111‑1 (MQ=255)                                                                                                         ttATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                               >  1:266016/1‑111 (MQ=255)                                                                                                            tGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAg                <  1:328940/139‑1 (MQ=255)                                                                                                                  aaCACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaata                                                                   <  2:880086/82‑1 (MQ=255)                                                                                                                  aaCACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaata                                                                   >  1:880086/1‑82 (MQ=255)                                                                                                                       ggCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                               >  2:860666/1‑97 (MQ=255)                                                                                                                       ggCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                               <  1:860666/97‑1 (MQ=255)                                                                                                                       ggCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTgg                                  >  1:799948/1‑110 (MQ=255)                                                                                                                       ggCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTgg                                  <  2:799948/110‑1 (MQ=255)                                                                                                                          gTTTGCGCGGGCGCCATCCAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCAcc  <  1:763536/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                              TTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACC  >  NC_000913/486507‑486766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑