Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	649,372	A→G	F67S (TTC→TCC)	citF ←	citrate lyase, citrate‑ACP transferase (alpha) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	649,372	0	A	G	100.0%	203.4 / NA	56	F67S (TTC→TCC)	citF	citrate lyase, citrate‑ACP transferase (alpha) subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/32);  total (24/32)

TGTTCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCA  >  NC_000913/649243‑649497                                                                                                                                  |                                                                                                                              tgtTCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCg                                                                                                                      <  1:237111/139‑1 (MQ=255)   ttCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTc                                                                                                                    >  1:220558/1‑139 (MQ=255)      tACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATg                                                                                                                 <  2:119368/139‑1 (MQ=255)      tACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATg                                                                                                                 <  2:432884/139‑1 (MQ=255)         cAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                              <  1:747440/139‑1 (MQ=255)         cAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                              <  1:552340/139‑1 (MQ=255)         cAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                              <  2:220558/139‑1 (MQ=255)         cAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                              <  2:653537/139‑1 (MQ=255)           gcggcgCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATg                                                                                                                 >  1:1027010/1‑134 (MQ=255)           gcggcgCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATg                                                                                                                 <  2:1027010/134‑1 (MQ=255)           gcggcgCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTc                                                                                                            <  1:723469/139‑1 (MQ=255)             ggcgCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                              >  1:179513/1‑135 (MQ=255)             ggcgCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCg                                                                                                              <  2:179513/135‑1 (MQ=255)                  aTGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATg                                                                                                                 >  2:525600/1‑127 (MQ=255)                  aTGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATg                                                                                                                 <  1:525600/127‑1 (MQ=255)                   tGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAAc                                                                                                    <  1:110988/139‑1 (MQ=255)                         tCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATg                                                                                                                 >  2:1026682/1‑120 (MQ=255)                         tCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATg                                                                                                                 <  1:1026682/120‑1 (MQ=255)                            cTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGcgac                                                                                           >  2:842606/1‑139 (MQ=255)                              cAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATg                                                                                                                 >  1:511096/1‑115 (MQ=255)                              cAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATg                                                                                                                 <  2:511096/115‑1 (MQ=255)                               aGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGaa                                                                                        >  1:56741/1‑139 (MQ=255)                               aGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGaa                                                                                        >  1:33779/1‑139 (MQ=255)                                    gCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGct                                                                                   <  2:322971/139‑1 (MQ=255)                                    gCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGct                                                                                   <  2:660273/139‑1 (MQ=255)                                               gggTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACGATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTACACCAGAGCGAGGAATCGCTTCTTCCAGGtt                                                                        >  2:789578/1‑139 (MQ=255)                                               gggTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGtt                                                                        >  1:992671/1‑139 (MQ=255)                                                     ggTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                                  <  1:842606/139‑1 (MQ=255)                                                     ggTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                                  <  2:992671/139‑1 (MQ=255)                                                     ggTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                                  <  1:535588/139‑1 (MQ=255)                                                          ttAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATgggagga                                                                                                                           <  1:557733/77‑1 (MQ=255)                                                          ttAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATgggagga                                                                                                                           >  2:557733/1‑77 (MQ=255)                                                                    tCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATc                                                                                      <  1:249321/104‑1 (MQ=255)                                                                    tCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATc                                                                                      >  2:249321/1‑104 (MQ=255)                                                                               aCGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAg                                        <  2:33779/139‑1 (MQ=255)                                                                                  tCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCACACAGTTTGCGATCGCGCGCTTTTTCAGCCt                                     <  1:44633/139‑1 (MQ=255)                                                                                  tCCATCACCATATAGACGGTCAGGTCACCGCCACGGAAAGCGAGATGGGAGGCAACCGTCATGCCTTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCt                                     >  2:1071102/1‑139 (MQ=255)                                                                                             atTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                                  >  1:860663/1‑99 (MQ=255)                                                                                             atTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                                  <  2:860663/99‑1 (MQ=255)                                                                                             atTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTATGc                          >  1:513498/1‑139 (MQ=255)                                                                                                 aCGGTCAGGTCACCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTg                      >  2:691399/1‑139 (MQ=255)                                                                                                    gtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATg                                                                                                                 >  2:38975/1‑45 (MQ=255)                                                                                                    gtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATg                                                                                                                 <  1:38975/45‑1 (MQ=255)                                                                                                    gtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCa                                                                                                  >  2:299459/1‑60 (MQ=255)                                                                                                    gtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCa                                                                                                  <  1:299459/60‑1 (MQ=255)                                                                                                    gtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                                  >  1:542833/1‑92 (MQ=255)                                                                                                    gtcaggtcaCCGCCACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGca                                                                  <  2:542833/92‑1 (MQ=255)                                                                                                                 cACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcgcgcg                                                 <  1:462049/96‑1 (MQ=255)                                                                                                                 cACGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATcgcgcg                                                 >  2:462049/1‑96 (MQ=255)                                                                                                                   cGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGc                                                                                    >  2:832241/1‑59 (MQ=255)                                                                                                                   cGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGc                                                                                    <  1:832241/59‑1 (MQ=255)                                                                                                                   cGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTgg                                   >  2:284606/1‑108 (MQ=255)                                                                                                                   cGGAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTgg                                   <  1:284606/108‑1 (MQ=255)                                                                                                                     gAAAGCGTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCa  <  1:691399/139‑1 (MQ=255)                                                                                                                           gTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGc                    >  1:443536/1‑115 (MQ=255)                                                                                                                           gTGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGc                    <  2:443536/115‑1 (MQ=255)                                                                                                                            tGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGt                              <  1:302451/104‑1 (MQ=255)                                                                                                                            tGATGGGAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGt                              >  2:302451/1‑104 (MQ=255)                                                                                                                                  |                                                                                                                              TGTTCTACCAGCGGCGCATGGCAATCACTCAGGGAGCTGGACGCCAGGGTCAGGTTTTTAAAGCCCATCTTCGCGATGACGTCCATCACCATATTGACGGTCAGGTCACCGCCACGGAAAGCGTGATGGAAGGAAACCGTCATGCCGTCCTGTAAACCAGAGCGACGAATCGCTTCTTCCAGGTTGGCGCACAGTTTGCGATCGCGCGCTTTTTCAGCCTGGTAGGTTTGCTTTGGCGAGTTCTGGAAAGCGGCA  >  NC_000913/649243‑649497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑