Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	50,044	G→A	W74* (TGG→TGA)	folA →	dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	50,044	0	G	A	100.0%	144.2 / NA	45	W74* (TGG→TGA)	folA	dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/23);  total (22/23)

CTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAA  >  NC_000913/49909‑50176                                                                                                                                        |                                                                                                                                     cTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCGATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTg                                                                                                                                   <  2:695486/139‑1 (MQ=255)        aaaCGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCgg                                                                                                                            <  1:706141/139‑1 (MQ=255)        aaaCGCAACACCTTAAATAAACCCGTGATTATAGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCgg                                                                                                                            <  2:117673/139‑1 (MQ=255)                           aaCCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCgcggc                                                                                                         <  1:386301/139‑1 (MQ=255)                                     tATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATcgcg                                                                                                           <  2:331746/127‑1 (MQ=255)                                     tATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATcgcg                                                                                                           >  1:331746/1‑127 (MQ=255)                                           ccgccATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCa                                                                                         >  1:766531/1‑139 (MQ=255)                                            cgccATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAg                                                                                        >  1:930591/1‑139 (MQ=255)                                              ccATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGt                                                                                                                               >  2:266019/1‑98 (MQ=255)                                              ccATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGt                                                                                                                               <  1:266019/98‑1 (MQ=255)                                                      gggAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGt                                                                              >  2:174638/1‑139 (MQ=255)                                                             aatcGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACg                                                                                              <  2:550439/116‑1 (MQ=255)                                                             aatcGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACg                                                                                              >  1:550439/1‑116 (MQ=255)                                                             aatcGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTggc                                                                       >  2:230181/1‑139 (MQ=255)                                                                       ccGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTcgcg                                                             <  2:766531/139‑1 (MQ=255)                                                                             ccAGGACGCAAAAATATTATCCTCAGCAGTCAACCGTGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATg                                                       >  2:190308/1‑139 (MQ=255)                                                                             ccAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATg                                                       >  2:334054/1‑139 (MQ=255)                                                                                 gACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTgtggtg                                                                                                 >  1:28935/1‑93 (MQ=255)                                                                                 gACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTgtggtg                                                                                                 <  2:28935/93‑1 (MQ=255)                                                                                 gACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACa                                                   >  2:345832/1‑139 (MQ=255)                                                                                       aaaaTATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCa                                                                                  >  2:257892/1‑102 (MQ=255)                                                                                       aaaaTATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCa                                                                                  <  1:257892/102‑1 (MQ=255)                                                                                              tatCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCaaaa                                      >  1:843094/1‑139 (MQ=255)                                                                                                tCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGttt                                                          <  1:479542/117‑1 (MQ=255)                                                                                                tCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGttt                                                          >  2:479542/1‑117 (MQ=255)                                                                                                  cTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAgcgc                                  <  2:843094/139‑1 (MQ=255)                                                                                                   tCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCa                                 <  1:174638/139‑1 (MQ=255)                                                                                                     agcagTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATcgcg                                                                                                           >  2:390059/1‑63 (MQ=255)                                                                                                     agcagTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATcgcg                                                                                                           <  1:390059/63‑1 (MQ=255)                                                                                                      gcagTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaaa                              <  2:930591/139‑1 (MQ=255)                                                                                                           cAACCGGGTACGGACGATCGCGTAACGTGAGTGACGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCAGGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGt                         >  2:386330/1‑139 (MQ=255)                                                                                                            aaCCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATcgcg                                                                                                           >  1:816735/1‑56 (MQ=255)                                                                                                            aaCCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATcgcg                                                                                                           <  2:816735/56‑1 (MQ=255)                                                                                                                gggTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTg                    <  1:230181/139‑1 (MQ=255)                                                                                                                        aCGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAg                                                                                                                   >  2:105462/1‑36 (MQ=255)                                                                                                                        aCGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAg                                                                                                                   <  1:105462/36‑1 (MQ=255)                                                                                                                        aCGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCatat            <  2:71028/139‑1 (MQ=255)                                                                                                                        aCGATCGCGTAACCTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACTGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCatat            <  2:748556/139‑1 (MQ=255)                                                                                                                            tCGCGTAACGTGAGCGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGAc        >  2:199408/1‑139 (MQ=255)                                                                                                                             cgcgTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAg                                                                                        >  2:72354/1‑58 (MQ=255)                                                                                                                             cgcgTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAg                                                                                        <  1:72354/58‑1 (MQ=255)                                                                                                                             cgcgTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACg       <  1:334054/139‑1 (MQ=255)                                                                                                                                 tAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTgtggtg                                                                                                 <  2:615876/45‑1 (MQ=255)                                                                                                                                 tAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTgtggtg                                                                                                 >  1:615876/1‑45 (MQ=255)                                                                                                                                  aaCGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGaa  >  2:744015/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                     CTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAA  >  NC_000913/49909‑50176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑