Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	679,874	G→A	G123R (GGG→AGG)	ybeU →	DUF1266 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	679,874	0	G	A	100.0%	200.5 / NA	62	G123R (GGG→AGG)	ybeU	DUF1266 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/34);  total (28/34)

GCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAG  >  NC_000913/679740‑680009                                                                                                                                       |                                                                                                                                        gCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGtt                                                                                                                                     <  2:506403/139‑1 (MQ=255)  ctctTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGttt                                                                                                                                    <  1:813124/139‑1 (MQ=255)            gAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGTGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGTATTATGTTCGAATGAGGTTTCTGTCACggg                                                                                                                          <  1:282211/139‑1 (MQ=255)             aaGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGttt                                                                                                                                    <  1:830777/128‑1 (MQ=255)             aaGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGttt                                                                                                                                    >  2:830777/1‑128 (MQ=255)             aaGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATGATGTTCGAATGAGGTTTCTGTCACGGGt                                                                                                                         >  2:1020695/1‑139 (MQ=255)                 gTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGgt                                                                                                                     <  1:509830/139‑1 (MQ=255)                  tGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGgtg                                                                                                                    <  1:461645/139‑1 (MQ=255)                  tGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGgtg                                                                                                                    <  1:524142/139‑1 (MQ=255)                  tGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGgtg                                                                                                                    >  2:461645/1‑139 (MQ=255)                  tGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGgtg                                                                                                                    >  2:240162/1‑139 (MQ=255)                          aTTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTcaac                                                                                                            <  1:240162/139‑1 (MQ=255)                                    ggtggCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGa                                                                                                  <  1:919431/139‑1 (MQ=255)                                        gCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTc                                                                                                                               <  1:869325/106‑1 (MQ=255)                                        gCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTc                                                                                                                               >  2:869325/1‑106 (MQ=255)                                         cTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCg                                                                                                                       <  2:652639/113‑1 (MQ=255)                                         cTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCg                                                                                                                       >  1:652639/1‑113 (MQ=255)                                          tCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGgaag                                                                                            >  2:667527/1‑139 (MQ=255)                                            ggAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGgaagaa                                                                                          <  1:667527/139‑1 (MQ=255)                                              agagagGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAagaaga                                                                                        <  1:947391/139‑1 (MQ=255)                                                       tGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATg                                                                               >  1:1002614/1‑139 (MQ=255)                                                            tataCCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGc                                                                          >  2:79142/1‑139 (MQ=255)                                                              taCCCAATTCTTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGATTGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATCGcgtcaa                                                                        >  2:1027202/1‑134 (MQ=39)                                                              taCCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGgtg                                                                                                                    >  2:170035/1‑95 (MQ=255)                                                              taCCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGgtg                                                                                                                    <  1:170035/95‑1 (MQ=255)                                                              taCCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCaa                                                                        >  1:422276/1‑139 (MQ=255)                                                                   aaTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcg                                                                   >  1:368131/1‑139 (MQ=255)                                                                   aaTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcg                                                                   >  1:368130/1‑139 (MQ=255)                                                                   aaTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcg                                                                   <  1:169166/139‑1 (MQ=255)                                                                    aTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcgc                                                                  >  1:372259/1‑139 (MQ=255)                                                                     ttCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAagaaga                                                                                        >  1:352448/1‑116 (MQ=255)                                                                     ttCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAagaaga                                                                                        <  2:352448/116‑1 (MQ=255)                                                                             ccAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTa                                                         <  1:1049652/139‑1 (MQ=255)                                                                                 aCCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAg                                                     <  2:422276/139‑1 (MQ=255)                                                                                      tATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                                                >  1:181109/1‑123 (MQ=255)                                                                                      tATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                                                <  2:181109/123‑1 (MQ=255)                                                                                             tgtgGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTca                                                                                                              <  2:570480/70‑1 (MQ=255)                                                                                             tgtgGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTca                                                                                                              >  1:570480/1‑70 (MQ=255)                                                                                               tgGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTc                                                  <  1:288788/128‑1 (MQ=255)                                                                                               tgGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTc                                                  >  2:288788/1‑128 (MQ=255)                                                                                               tgGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTAt                                       <  2:372259/139‑1 (MQ=255)                                                                                                  ggAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATcac                                    >  1:616283/1‑139 (MQ=255)                                                                                                    aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAg                                  >  1:1027783/1‑139 (MQ=255)                                                                                                      gggggAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCa                                                                                                                              >  1:1003164/1‑45 (MQ=255)                                                                                                      gggggAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCa                                                                                                                              <  2:1003164/45‑1 (MQ=255)                                                                                                      gggggAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGt                                                                                    >  2:919289/1‑87 (MQ=255)                                                                                                      gggggAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGt                                                                                    <  1:919289/87‑1 (MQ=255)                                                                                                        gggAATCAACGCCTGGGATTATGTTCCAATGAGGTTGCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGACGAAGAGAGTTTATGGCTGAAATCGCGAGTCTATGTACGAGCTAACCATTACTATCACAGCTgg                              <  1:1027202/139‑1 (MQ=255)                                                                                                         ggAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGa                                                                                                  <  1:346585/70‑1 (MQ=255)                                                                                                         ggAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGa                                                                                                  >  2:346585/1‑70 (MQ=255)                                                                                                           aaTCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATg                           <  1:79142/139‑1 (MQ=255)                                                                                                           aaTCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATg                           <  1:267382/139‑1 (MQ=255)                                                                                                           aaTCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATg                           <  2:878435/139‑1 (MQ=255)                                                                                                                       ggATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTACTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTcc               >  2:68121/1‑139 (MQ=255)                                                                                                                             tGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTAt         <  2:368131/139‑1 (MQ=255)                                                                                                                             tGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTAt         <  2:368130/139‑1 (MQ=255)                                                                                                                                 cGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGt     >  1:257539/1‑139 (MQ=255)                                                                                                                                  gAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTcc               <  1:841839/128‑1 (MQ=255)                                                                                                                                  gAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTcc               >  2:841839/1‑128 (MQ=255)                                                                                                                                   aaTGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCAt                                             >  1:236692/1‑97 (MQ=255)                                                                                                                                   aaTGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCAt                                             <  2:236692/97‑1 (MQ=255)                                                                                                                                    aTGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAg  <  2:104579/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        GCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTATTCGTTAG  >  NC_000913/679740‑680009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑