Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 746,726 G→A *264* (TAG→TAA
A348A (GCC→GCT
nei →
abrB ←
endonuclease VIII and 5‑formyluracil/5‑hydroxymethyluracil DNA glycosylase
regulator of aidB expression, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913746,7260GA100.0% 120.5 / NA 38*264* (TAG→TAA
A348A (GCC→GCT
nei
abrB
endonuclease VIII and 5‑formyluracil/5‑hydroxymethyluracil DNA glycosylase
regulator of aidB expression, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/20);  total (18/20)

GCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATAT  >  NC_000913/746594‑746832
                                                                                                                                    |                                                                                                          
gCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGAc                                                                                                      >  2:198613/1‑139 (MQ=255)
   tcatGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGc                                                                                                   >  2:908609/1‑139 (MQ=255)
     atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGAc                                                                                                      <  2:232552/134‑1 (MQ=255)
     atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGAc                                                                                                      >  1:232552/1‑134 (MQ=255)
     atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCtt                                                                                                 >  2:168288/1‑139 (MQ=255)
     atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCtt                                                                                                 >  1:1055428/1‑139 (MQ=255)
     atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCtt                                                                                                 >  1:2903/1‑139 (MQ=255)
     atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCtt                                                                                                 >  2:715028/1‑139 (MQ=255)
          gcgcTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGgcg                                                                                            >  2:908760/1‑139 (MQ=255)
              tGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGTTGCCAGCATTAAGCCGACCGCTTCGGCGCATa                                                                                        <  2:674686/139‑1 (MQ=255)
                               tttCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACcgcg                                                                       <  2:276113/139‑1 (MQ=255)
                                 tCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCa                                                                     <  1:168288/139‑1 (MQ=255)
                                           ggCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGcc                                                           <  1:715028/139‑1 (MQ=255)
                                             cGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCt                                                         <  1:288824/139‑1 (MQ=255)
                                                                   agcaTCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGc                                   >  2:188046/1‑139 (MQ=255)
                                                                    gcaTCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGt                                                       >  2:881068/1‑118 (MQ=255)
                                                                    gcaTCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGt                                                       <  1:881068/118‑1 (MQ=255)
                                                                     catcatTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCa                                                  >  1:150311/1‑122 (MQ=255)
                                                                     catcatTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCa                                                  <  2:150311/122‑1 (MQ=255)
                                                                          ttGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGt                            <  1:188046/139‑1 (MQ=255)
                                                                               aaaaCCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCa                                                                                          <  2:1081046/72‑1 (MQ=255)
                                                                               aaaaCCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCa                                                                                          >  1:1081046/1‑72 (MQ=255)
                                                                               aaaaCCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCa                       <  1:198613/139‑1 (MQ=255)
                                                                                  aCCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAAc                                                                           <  2:915807/84‑1 (MQ=255)
                                                                                  aCCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAAc                                                                           >  1:915807/1‑84 (MQ=255)
                                                                                       gCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGt                                                       <  2:467340/99‑1 (MQ=255)
                                                                                       gCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGt                                                       >  1:467340/1‑99 (MQ=255)
                                                                                       gCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATa               <  2:1055428/139‑1 (MQ=255)
                                                                                            cATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATATGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCTTAATGAg          <  2:1025851/139‑1 (MQ=255)
                                                                                                   cccGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGcc                                                           >  2:1098786/1‑83 (MQ=255)
                                                                                                   cccGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGcc                                                           <  1:1098786/83‑1 (MQ=255)
                                                                                                    ccGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCatat  <  1:908760/139‑1 (MQ=255)
                                                                                                     cGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                        >  2:724597/1‑100 (MQ=255)
                                                                                                     cGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                        <  1:724597/100‑1 (MQ=255)
                                                                                                                          gCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACcgcg                                                                       <  2:281808/48‑1 (MQ=255)
                                                                                                                          gCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACcgcg                                                                       >  1:281808/1‑48 (MQ=255)
                                                                                                                            cAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                        <  1:819762/77‑1 (MQ=255)
                                                                                                                            cAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                        >  2:819762/1‑77 (MQ=255)
                                                                                                                                    |                                                                                                          
GCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATAT  >  NC_000913/746594‑746832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: