Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 758,907 C→T R68C (CGC→TGC)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913758,9070CT100.0% 163.7 / NA 50R68C (CGC→TGC) sucA2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/23);  total (27/23)

CTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCCGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTG  >  NC_000913/758781‑759041
                                                                                                                              |                                                                                                                                      
ctctATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAg                                                                                                                            >  1:99277/1‑139 (MQ=255)
ctctATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAg                                                                                                                            >  1:802993/1‑139 (MQ=255)
ctctATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAg                                                                                                                            >  1:142531/1‑139 (MQ=255)
   tATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACg                                                                                                                         <  2:142531/139‑1 (MQ=255)
     tGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCt                                                                                                                       <  1:1108538/139‑1 (MQ=255)
          aCTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCt                                                                                                                       >  2:126686/1‑134 (MQ=255)
          aCTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCt                                                                                                                       <  1:126686/134‑1 (MQ=255)
           cttcttAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGt                                                                                                                 <  1:558030/139‑1 (MQ=255)
               ttAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACg                                                                                                                  >  1:852130/1‑134 (MQ=255)
               ttAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACg                                                                                                                  <  2:852130/134‑1 (MQ=255)
                        ccTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGa                                                                                                    >  1:1128894/1‑139 (MQ=255)
                           gACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGaaa                                                                                                                             <  1:383523/111‑1 (MQ=255)
                           gACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGaaa                                                                                                                             >  2:383523/1‑111 (MQ=255)
                                ggTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGAc                                                                                            >  2:921029/1‑139 (MQ=255)
                                 gTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAATGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGAcc                                                                                           >  2:811922/1‑139 (MQ=255)
                                 gTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCa                                                                                                                    >  2:292749/1‑114 (MQ=255)
                                 gTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCa                                                                                                                    <  1:292749/114‑1 (MQ=255)
                                 gTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGAcc                                                                                           >  2:687308/1‑139 (MQ=255)
                                 gTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGAcc                                                                                           >  2:574829/1‑139 (MQ=255)
                                  ttGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGAccc                                                                                          >  2:166690/1‑139 (MQ=255)
                                        cTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGacac                                                                                    <  2:1128894/139‑1 (MQ=255)
                                        cTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGacac                                                                                    <  1:856521/139‑1 (MQ=255)
                                              ggCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAAtgt                                                                              >  2:987743/1‑139 (MQ=255)
                                                                 gTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTg                                                           <  1:166690/139‑1 (MQ=255)
                                                                    aCCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCtt                                                                                                                      <  1:96619/77‑1 (MQ=255)
                                                                    aCCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCtt                                                                                                                      >  2:96619/1‑77 (MQ=255)
                                                                     ccTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGc                                                       <  1:987743/139‑1 (MQ=255)
                                                                               gAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTTCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGc                                             >  1:516219/1‑139 (MQ=255)
                                                                               gAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGc                                             >  2:994907/1‑139 (MQ=255)
                                                                                   cAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTaa                                                >  2:692769/1‑132 (MQ=255)
                                                                                   cAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTaa                                                <  1:692769/132‑1 (MQ=255)
                                                                                   cAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATAc                                         >  2:831414/1‑139 (MQ=255)
                                                                                       ccGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCt                                     >  2:796736/1‑139 (MQ=255)
                                                                                           aTCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAAtgtg                                                                             <  2:204895/95‑1 (MQ=255)
                                                                                           aTCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAAtgtg                                                                             >  1:204895/1‑95 (MQ=255)
                                                                                               aTTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTTCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGt                             <  2:516219/139‑1 (MQ=255)
                                                                                                ttCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGaca                                                                                     <  2:458721/82‑1 (MQ=255)
                                                                                                ttCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGaca                                                                                     >  1:458721/1‑82 (MQ=255)
                                                                                                ttCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTc                            <  2:968166/139‑1 (MQ=255)
                                                                                                  ccACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCAc                          <  1:574829/139‑1 (MQ=255)
                                                                                                  ccACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCAc                          <  2:99277/139‑1 (MQ=255)
                                                                                                     ctctCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACg                                                                                                                  >  2:363964/1‑48 (MQ=255)
                                                                                                     ctctCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACg                                                                                                                  <  1:363964/48‑1 (MQ=255)
                                                                                                        tcAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCg                                                                                              <  1:815519/65‑1 (MQ=255)
                                                                                                        tcAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCg                                                                                              >  2:815519/1‑65 (MQ=255)
                                                                                                         cAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATg                   >  1:649339/1‑139 (MQ=255)
                                                                                                          aaaCGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCa                                            <  2:480605/113‑1 (MQ=255)
                                                                                                          aaaCGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCa                                            >  1:480605/1‑113 (MQ=255)
                                                                                                                  gAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCg          >  1:1082961/1‑139 (MQ=255)
                                                                                                                         tCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCt   <  1:994907/139‑1 (MQ=255)
                                                                                                                          ccgctgCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTg  >  1:81885/1‑139 (MQ=255)
                                                                                                                              |                                                                                                                                      
CTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCCGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTG  >  NC_000913/758781‑759041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑