Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 913,349 G→A L156L (CTG→TTG)  hcp ← hybrid‑cluster [4Fe‑2S‑2O] subunit of anaerobic terminal reductases

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913913,3490GA100.0% 121.2 / NA 38L156L (CTG→TTG) hcphybrid‑cluster [4Fe‑2S‑2O] subunit of anaerobic terminal reductases
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/16);  total (22/16)

CGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGAC  >  NC_000913/913220‑913484
                                                                                                                                 |                                                                                                                                       
cgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCAcagcag                                                                                                                                >  1:843369/1‑139 (MQ=255)
                              aCGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCgaga                                                                                                                   <  2:628265/122‑1 (MQ=255)
                              aCGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCgaga                                                                                                                   >  1:628265/1‑122 (MQ=255)
                                                                   gTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGtt                                                                                   <  1:204487/117‑1 (MQ=255)
                                                                   gTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGtt                                                                                   >  2:204487/1‑117 (MQ=255)
                                                                     cGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgccg                                                                                                >  2:590636/1‑102 (MQ=255)
                                                                     cGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgccg                                                                                                <  1:590636/102‑1 (MQ=255)
                                                                      gTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGt                                                          >  2:309920/1‑139 (MQ=255)
                                                                            aCCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGc                                                    >  1:192348/1‑139 (MQ=255)
                                                                              cGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAg                                                     >  2:349615/1‑136 (MQ=255)
                                                                              cGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAg                                                     <  1:349615/136‑1 (MQ=255)
                                                                              cGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTc                                                  >  1:1071508/1‑139 (MQ=255)
                                                                                         gcgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAtcgt                                       <  2:1071508/139‑1 (MQ=255)
                                                                                               tCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCa                                 <  1:470528/139‑1 (MQ=255)
                                                                                                        gccgccGCACCTTTCAGGCCATACAAGCACAGCAGACGCAgg                                                                                                                         <  1:862287/42‑1 (MQ=255)
                                                                                                        gccgccGCACCTTTCAGGCCATACAAGCACAGCAGACGCAgg                                                                                                                         >  2:862287/1‑42 (MQ=255)
                                                                                                        gccgccGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGcc                                                                                                                       <  1:461984/44‑1 (MQ=255)
                                                                                                        gccgccGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGcc                                                                                                                       >  2:461984/1‑44 (MQ=255)
                                                                                                        gccgccGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCGATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCa                        >  1:641712/1‑139 (MQ=255)
                                                                                                        gccgccGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTg                          >  1:988520/1‑137 (MQ=255)
                                                                                                        gccgccGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTg                          <  2:988520/137‑1 (MQ=255)
                                                                                                        gccgccGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCa                        >  2:883186/1‑139 (MQ=255)
                                                                                                        gccgccGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCa                        >  1:95098/1‑139 (MQ=255)
                                                                                                             cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAg                                                     <  1:625011/105‑1 (MQ=255)
                                                                                                             cgcACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAg                                                     >  2:625011/1‑105 (MQ=255)
                                                                                                                    ttCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaaa                                                                          >  2:49470/1‑77 (MQ=255)
                                                                                                                    ttCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaaa                                                                          <  1:49470/77‑1 (MQ=255)
                                                                                                                          ccATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcg                                                                                                   >  2:991574/1‑46 (MQ=255)
                                                                                                                          ccATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcg                                                                                                   <  1:991574/46‑1 (MQ=255)
                                                                                                                          ccATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTAt      <  1:883186/139‑1 (MQ=255)
                                                                                                                           cATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCAc                                >  2:161697/1‑112 (MQ=255)
                                                                                                                           cATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCAc                                <  1:161697/112‑1 (MQ=255)
                                                                                                                            aTACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgccg                                                                                                <  1:890503/47‑1 (MQ=255)
                                                                                                                            aTACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgccg                                                                                                >  2:890503/1‑47 (MQ=255)
                                                                                                                            aTACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGt                     <  2:266818/122‑1 (MQ=255)
                                                                                                                            aTACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGt                     >  1:266818/1‑122 (MQ=255)
                                                                                                                            aTACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCg    >  1:512385/1‑139 (MQ=255)
                                                                                                                              aCAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGAc  >  2:224380/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                       
CGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGAC  >  NC_000913/913220‑913484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑