Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	922,366	C→T	*75* (TAG→TAA)	cspD ←	inhibitor of DNA replication, cold shock protein homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	922,366	0	C	T	100.0%	150.9 / NA	47	*75* (TAG→TAA)	cspD	inhibitor of DNA replication, cold shock protein homolog
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/22);  total (25/22)

CTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAATGGT  >  NC_000913/922233‑922494                                                                                                                                      |                                                                                                                                 cTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGc                                                                                                                             >  1:1082273/1‑139 (MQ=255)      tACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTg                                                                                                                        >  2:37169/1‑139 (MQ=255)                     cGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGa                                                                                                         >  1:232294/1‑139 (MQ=255)                       gCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACg                                                                                                       <  2:991011/139‑1 (MQ=255)                                       tAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGTCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg                                                                                                     <  2:797500/125‑1 (MQ=255)                                       tAGATGCTCTGGCACGAGAGCAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGTCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg                                                                                                     >  1:797500/1‑125 (MQ=255)                                            gCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAaca                                                                                           <  2:1073130/130‑1 (MQ=255)                                            gCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAaca                                                                                           >  1:1073130/1‑130 (MQ=255)                                                 ggCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCg                                                                                                          >  2:856339/1‑110 (MQ=255)                                                 ggCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCg                                                                                                          <  1:856339/110‑1 (MQ=255)                                                 ggCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGcc                                                                             >  2:902334/1‑139 (MQ=255)                                                        gagTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg                                                                                                     <  2:251161/108‑1 (MQ=255)                                                        gagTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg                                                                                                     >  1:251161/1‑108 (MQ=255)                                                                   gagaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTg                                                                        >  2:456854/1‑126 (MQ=255)                                                                   gagaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTg                                                                        <  1:456854/126‑1 (MQ=255)                                                                                         gggCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg                                                                                                     <  1:669860/75‑1 (MQ=255)                                                                                         gggCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg                                                                                                     >  2:669860/1‑75 (MQ=255)                                                                                           gCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGc                                   >  1:1111540/1‑139 (MQ=255)                                                                                                  ttttGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGc                            <  1:37169/139‑1 (MQ=255)                                                                                                   tttGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCg                           <  2:1111540/139‑1 (MQ=255)                                                                                                    ttGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGt                          >  2:291084/1‑139 (MQ=255)                                                                                                     tGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGcccc                                                                   >  2:273247/1‑97 (MQ=255)                                                                                                     tGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGcccc                                                                   <  1:273247/97‑1 (MQ=255)                                                                                                     tGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGtt                         <  1:902334/139‑1 (MQ=255)                                                                                                              gATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAAcca                <  2:1082273/139‑1 (MQ=255)                                                                                                              gATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAAcca                <  1:291084/139‑1 (MQ=255)                                                                                                                  tACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGa                                                                                                         >  1:462469/1‑46 (MQ=255)                                                                                                                  tACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGa                                                                                                         <  2:462469/46‑1 (MQ=255)                                                                                                                  tACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAAccatcca            >  2:921983/1‑139 (MQ=255)                                                                                                                   acacAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg                                                                                                     >  1:262424/1‑49 (MQ=255)                                                                                                                   acacAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg                                                                                                     <  2:262424/49‑1 (MQ=255)                                                                                                                    cacaATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGc                                                                      >  2:993548/1‑79 (MQ=255)                                                                                                                    cacaATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGc                                                                      <  1:993548/79‑1 (MQ=255)                                                                                                                        gtGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAAccatc              >  1:697514/2‑131 (MQ=255)                                                                                                                        aTGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAAccatc              <  2:697514/131‑1 (MQ=255)                                                                                                                         tGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg                                                                                                     <  2:1110359/43‑1 (MQ=255)                                                                                                                         tGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg                                                                                                     >  1:1110359/1‑43 (MQ=255)                                                                                                                         tGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGa                                                                                  <  2:586967/62‑1 (MQ=255)                                                                                                                         tGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGa                                                                                  >  1:586967/1‑62 (MQ=255)                                                                                                                         tGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGc                                   <  2:276634/109‑1 (MQ=255)                                                                                                                         tGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGc                                   >  1:276634/1‑109 (MQ=255)                                                                                                                         tGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAAt     >  1:175075/1‑139 (MQ=255)                                                                                                                            gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAAtggt  >  2:607741/1‑139 (MQ=255)                                                                                                                               agaagaGTTATGCGACTGCCGCTTCTACTTCGACg                                                                                                       >  2:464534/1‑35 (MQ=38)                                                                                                                               agaagaGTTATGCGACTGCCGCTTCTACTTCGACg                                                                                                       <  1:464534/35‑1 (MQ=38)                                                                                                                               agaagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGaa      <  2:422898/132‑1 (MQ=255)                                                                                                                               agaagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGaa      >  1:422898/1‑132 (MQ=255)                                                                                                                                      |                                                                                                                                 CTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAATGGT  >  NC_000913/922233‑922494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑