Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 927,205 A→G R76R (CGT→CGC)  aat ← leucyl/phenylalanyl‑tRNA‑protein transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913927,2050AG100.0% 140.5 / NA 41R76R (CGT→CGC) aatleucyl/phenylalanyl‑tRNA‑protein transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/22);  total (19/22)

ATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGC  >  NC_000913/927070‑927327
                                                                                                                                       |                                                                                                                          
aTGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCgg                                                                                                                         <  1:359245/139‑1 (MQ=255)
           cGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg                                                                                                              >  1:648847/1‑139 (MQ=255)
             aCCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTg                                                                                                            >  1:367118/1‑139 (MQ=255)
                acgccacgcGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGAtt                                                                                                         <  2:245730/139‑1 (MQ=255)
                    cacgcGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCGCAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTgg                                                                                                     <  2:723847/139‑1 (MQ=255)
                       gcgTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCa                                                                                                  >  1:803872/1‑139 (MQ=255)
                            aTCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCa                                                                                             >  2:1121431/1‑139 (MQ=255)
                             tCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCAc                                                                                            <  1:416259/139‑1 (MQ=255)
                                 ggTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACcgcg                                                                                        <  1:128839/139‑1 (MQ=255)
                                 ggTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACcgcg                                                                                        <  2:1122993/139‑1 (MQ=255)
                                   ttCCTTCTTCGCGATCGCTGGCAC‑GCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCgg                                                                                     >  2:615423/1‑139 (MQ=255)
                                    tCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCgg                                                                                     >  2:593790/1‑139 (MQ=255)
                                       ttcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCa                                                                                             >  2:252150/1‑128 (MQ=255)
                                       ttcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCa                                                                                             <  1:252150/128‑1 (MQ=255)
                                       ttcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGa                                                                                  >  2:246178/1‑139 (MQ=255)
                                         cttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATc                                                                                <  1:547417/139‑1 (MQ=255)
                                                aTCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGac                                                                         <  1:259901/139‑1 (MQ=255)
                                                            aagagacaggacctgaccAAAAGCGTAATTCATCGTGACACGTTAGGGCGGGCGGTTATGAAAGCGCAGCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGGGCGGGGATGGGGCGACCACCAGAGGATg                                                             <  2:667308/130‑1 (MQ=255)
                                                                            ccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCAGCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGaaa                                             >  1:676532/1‑139 (MQ=255)
                                                                                aaGCGAAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTggctgtctctc                                         >  1:667308/1‑130 (MQ=255)
                                                                                     tAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCggg                                                                                    >  1:537016/1‑91 (MQ=255)
                                                                                     tAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCggg                                                                                    <  2:537016/91‑1 (MQ=255)
                                                                                     tAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGaa                                    <  1:107240/139‑1 (MQ=255)
                                                                                             cGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg                                                                                                              <  1:394816/57‑1 (MQ=255)
                                                                                             cGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg                                                                                                              >  2:394816/1‑57 (MQ=255)
                                                                                                gACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg                                                                                                              <  1:60627/54‑1 (MQ=255)
                                                                                                gACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg                                                                                                              >  2:60627/1‑54 (MQ=255)
                                                                                                          ggCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGAccacca                                                                    <  1:748595/86‑1 (MQ=255)
                                                                                                          ggCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCa               >  1:966339/1‑139 (MQ=255)
                                                                                                          ggCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCa               >  2:844542/1‑139 (MQ=255)
                                                                                                          ggCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCAATAGAACCGCGCGGGGATCGGGCGAccacca                                                                    >  2:748595/1‑86 (MQ=255)
                                                                                                           gCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCAt              >  2:957822/1‑139 (MQ=255)
                                                                                                            cGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCAtt             <  1:246178/139‑1 (MQ=255)
                                                                                                              aGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTTGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTaa           <  2:367118/139‑1 (MQ=255)
                                                                                                                    tATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAg                                                                                               >  1:630789/1‑49 (MQ=255)
                                                                                                                    tATGAAATCGCTTCATACTGCGGCTGATATCCAGTGATTCTGGCCATAg                                                                                               <  2:630789/49‑1 (MQ=255)
                                                                                                                      tGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcg   <  2:803872/139‑1 (MQ=255)
                                                                                                                       gAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc  <  1:615423/139‑1 (MQ=255)
                                                                                                                       gAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc  <  1:1121431/139‑1 (MQ=255)
                                                                                                                            cGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc  >  1:764308/1‑134 (MQ=255)
                                                                                                                            cGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc  <  2:764308/134‑1 (MQ=255)
                                                                                                                                       |                                                                                                                          
ATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGC  >  NC_000913/927070‑927327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑