Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	937,983	G→A	*204* (TAG→TAA)	lolA →	lipoprotein chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	937,983	0	G	A	100.0%	37.1 / NA	13	*204* (TAG→TAA)	lolA	lipoprotein chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/8);  total (5/8)

TTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAACATTTGCTGGCT  >  NC_000913/937852‑938101                                                                                                                                    |                                                                                                                       tttAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAAAGGCAcc                                                                                                                 <  2:1079514/139‑1 (MQ=255)                                                     aGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAAAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTgg                                                             >  1:172406/1‑139 (MQ=255)                                                      gCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAAAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGc                                                            <  2:463282/139‑1 (MQ=255)                                                                               aaaTTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAAAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataat                                                                             <  1:541126/97‑1 (MQ=255)                                                                               aaaTTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAAAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataat                                                                             >  2:541126/1‑97 (MQ=255)                                                                                                         cGTCACGGTAGATGATCAACGTAAGTAAAGGCACCTGAGTGAGCAATCTg                                                                                                  <  1:941600/50‑1 (MQ=255)                                                                                                         cGTCACGGTAGATGATCAACGTAAGTAAAGGCACCTGAGTGAGCAATCTg                                                                                                  >  2:941600/1‑50 (MQ=255)                                                                                                            cACGGTAGATGATCAACGTAAGTAAAGGCACCTGAGTGAGc                                                                                                        >  1:159483/1‑41 (MQ=255)                                                                                                            cACGGTAGATGATCAACGTAAGTAAAGGCACCTGAGTGAGc                                                                                                        <  2:159483/41‑1 (MQ=255)                                                                                                            cACGGTAGATGATCAACGTAAGTAAAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAACATTTgct      <  2:172406/139‑1 (MQ=255)                                                                                                                gTAGATGATCAACGTAAGTAAAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAACATTTgctggct  <  2:209910/139‑1 (MQ=255)                                                                                                                   gatgatCAACGTAAGTAAAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGcaca                              <  1:130829/108‑1 (MQ=255)                                                                                                                   gatgatCAACGTAAGTAAAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGcaca                              >  2:130829/1‑108 (MQ=255)                                                                                                                                    |                                                                                                                       TTTAGCGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACAGTATATCGGCCAGCAACATTTGCTGGCT  >  NC_000913/937852‑938101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑