Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,082,060 A→G pseudogene (601/726 nt) efeU → ferrous iron permease (pseudogene)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,082,0600AG100.0% 111.1 / NA 32pseudogene (601/726 nt)efeUferrous iron permease (pseudogene)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/17);  total (15/17)

CGCCGCAGGGCTGGCAGCTGGTGCCATTCGCGCATTTCATGAAGCCGGATTGTGGAACCACTTTCAGGAAATCGCCTTCGATATGAGTGCGGTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGATTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTGGTTTATTTATCTCATCCCGGCGCTGGTGGCATTTGCTCTGCCACCACGCGCAGGGGCGACAGCGTCTCGCTCCGCGT  >  NC_000913/1081927‑1082183
                                                                                                                                     |                                                                                                                           
cgccgcAGGGCTGGCAGCTGGTGCCATTCGCGCATTTCATGAAGCCGGATTGTGGAACCACTTTCAGGAAATCGCCTTCGATATGAGTGCGGTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGGttttt                                                                                                                        <  1:453570/139‑1 (MQ=255)
cgccgcAGGGCTGGCAGCTGGTGCCATTCGCGCATTTCATGAAGCCGGATTGTGGAACCACTTTCAGGAAATCGCCTTCGATATGAGTGCGGTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGGttttt                                                                                                                        >  1:64561/1‑139 (MQ=255)
                     tGCCATTCGCGCATTTCATGAAGCCGGATTGTGTAACCACTTTCAGGAAATCGCCTGCGATATGAGTGCGGTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGc                                                                                                   <  2:64561/139‑1 (MQ=255)
                         aTTCGCGCATTTCATGAAGCCGGATTGTGGAACCACTTTCAGGAAATCGCCTTCGATATGAGTGCGGTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTc                                                                                                >  1:256868/1‑138 (MQ=255)
                         aTTCGCGCATTTCATGAAGCCGGATTGTGGAACCACTTTCAGGAAATCGACTTCGATATGAGTGCGGTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTc                                                                                                <  2:256868/138‑1 (MQ=255)
                                      aTGAAGCCGGATTGTGGAACCACTTTCAGGAAATCGCCTTCGATATGAGTGCGGTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGt                                                                                  <  2:947219/139‑1 (MQ=255)
                                                              ttCAGGAAATCGCCTTCGATATGAGTGCGGTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTGGTTTATTTATCTCATCCCGgc                                                          >  1:879924/1‑139 (MQ=255)
                                                                      aTCGCCTTCGATATGAGTGCGGTGCTCTCAACTCCCTCGCTGTTTGGCACGCTGATGGAAGGGGtttt                                                                                                                         <  2:906487/68‑1 (MQ=255)
                                                                      aTCGCCTTCGATATGAGTGCGGTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGGtttt                                                                                                                         >  1:906487/1‑68 (MQ=255)
                                                                       tCGCCTTCGATATGAGTGCGGTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTGGTTTATTTATCTCATCCCGGCGCTGGTGGc                                                 >  1:776654/1‑139 (MQ=255)
                                                                          ccTTCGATATGAGTGCGGTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTGGTTTATTTATCTCATCCCGGCGCTGGTGGCAtt                                              <  2:776654/139‑1 (MQ=255)
                                                                                atatGAGTGCGGTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCg                                                                                                      <  2:701598/77‑1 (MQ=255)
                                                                                atatGAGTGCGGTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCg                                                                                                      >  1:701598/1‑77 (MQ=255)
                                                                                         cGGTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTGGTTTATTTATCTCATCCCGGCGCTGGTGGCATTTGCTCTGCCACCAcg                               >  2:194308/1‑139 (MQ=255)
                                                                                          ggTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTGGTttatttat                                                                     >  2:291643/1‑100 (MQ=255)
                                                                                          ggTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTGGTttatttat                                                                     <  1:291643/100‑1 (MQ=255)
                                                                                              ctctCAACTCACTCGCTGTTTGGCTCGCTGATGGAAGGGGTTTTTGGCTATc                                                                                                                 >  2:769154/1‑52 (MQ=255)
                                                                                              ctctCAACTCACTCGCTGTTTGGCACGCTGACGGAAGGGGTTTTTGGCTATc                                                                                                                 <  1:769154/52‑1 (MQ=255)
                                                                                                   aaCTCACTCGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCg                                                                                            <  2:335054/68‑1 (MQ=255)
                                                                                                   aaCTCACTCGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCg                                                                                            >  1:335054/1‑68 (MQ=255)
                                                                                                    actcactcGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTGGTTTATTTATCTCATCCCGGCGCTGGTGGCATTTGCTCTGCCACCACGCGCAGGGGCGa                    <  2:856786/139‑1 (MQ=255)
                                                                                                      tcactcGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTGGTTTATTTATCTCATCCCGGCGCTGGTGGCATTTGCTCTGCCACCACGCGCAGGGGCGACa                  <  2:337519/139‑1 (MQ=255)
                                                                                                        actcGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTgg                                                                              >  1:593561/1‑77 (MQ=255)
                                                                                                        actcGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTgg                                                                              <  2:593561/77‑1 (MQ=255)
                                                                                                        actcGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTGGTTTATTTATCTCATCCCGGCGCTGGTGGCATTTGCTCTGCCACCACGCGCAGGGGCGACAGc                >  2:46756/1‑139 (MQ=255)
                                                                                                          tcGCTGTTTGGCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTGGTTTATTTATCTCATCCCGGCGCTGGTGGCATTTGCTCTGCCACCACGCGCAGGGGCGACAGCGt              >  1:1045255/1‑139 (MQ=255)
                                                                                                                    gCACGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTGGTTTATTTATCTCATCCCGGCGCTGGTGGCATTTGCTCTGCCACCACGCGCAGGGGCGACAGCGTCTCGCTCcgc    <  1:194308/139‑1 (MQ=255)
                                                                                                                      aCGCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTGGTTTATTTATCTCATCCCGGCGCTGGTGGCATTTGCTCTGCCACCACGCGCAGGGGCGACAGCGTCTCGCTCCGCGt  <  2:879924/139‑1 (MQ=255)
                                                                                                                        gCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCt                                                                                <  1:388325/59‑1 (MQ=255)
                                                                                                                        gCTGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCt                                                                                >  2:388325/1‑59 (MQ=255)
                                                                                                                          tGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTGGTTTATTTATCTCATCCCGGCGCTGGTGGCATTTGCTCTGCCACCACgcgc                            <  1:186726/109‑1 (MQ=255)
                                                                                                                          tGATGGAAGGGGTTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTGGTTTATTTATCTCATCCCGGCGCTGGTGGCATTTGCTCTGCCACCACgcgc                            >  2:186726/1‑109 (MQ=255)
                                                                                                                                     |                                                                                                                           
CGCCGCAGGGCTGGCAGCTGGTGCCATTCGCGCATTTCATGAAGCCGGATTGTGGAACCACTTTCAGGAAATCGCCTTCGATATGAGTGCGGTGCTCTCAACTCACTCGCTGTTTGGCACGCTGATGGAAGGGATTTTTGGCTATCAGGAAGCGCCGAGCGTCAGCGAAGTCGCCGTCTGGTTTATTTATCTCATCCCGGCGCTGGTGGCATTTGCTCTGCCACCACGCGCAGGGGCGACAGCGTCTCGCTCCGCGT  >  NC_000913/1081927‑1082183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: